Molecular architecture of a galactoglucan from Rhizobium meliloti

Rhizobium meliloti mutants produce a linear, acidic exopolysaccharide with alternating galactopyranosyl and glucopyranosyl units having (1 → 3) linkages. It has a 4,6- O-pyruvic cyclic acetal group on the α-galactosyl and 6- O-acetyl ester group on the β-glucosyl units. X-ray diffraction patterns fr...

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Veröffentlicht in:Carbohydrate research 1994-08, Vol.261 (2), p.279-295
Hauptverfasser: Chandrasekaran, Rengaswami, Lee, Eun J., Thailambal, Vadakkanthara G., Zevenhuizen, Ludovicus P.T.M.
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container_end_page 295
container_issue 2
container_start_page 279
container_title Carbohydrate research
container_volume 261
creator Chandrasekaran, Rengaswami
Lee, Eun J.
Thailambal, Vadakkanthara G.
Zevenhuizen, Ludovicus P.T.M.
description Rhizobium meliloti mutants produce a linear, acidic exopolysaccharide with alternating galactopyranosyl and glucopyranosyl units having (1 → 3) linkages. It has a 4,6- O-pyruvic cyclic acetal group on the α-galactosyl and 6- O-acetyl ester group on the β-glucosyl units. X-ray diffraction patterns from polycrystalline and well oriented specimens of its potassium salt indicate that the polymer forms a 2-fold helix of pitch 15.89 Å. The three-dimensional structure has been determined and refined by using the X-ray intensities and the linked-atom least-squares technique. The details of the antiparallel packing arrangement of two helices in an orthorhombic unit cell, a = 14.49, b = 9.79, and c = 15.89 Å, reveal that each disaccharide repeating unit is associated with one potassium and three water molecules. The helices are interconnected by a series of ⋯ COO − ⋯ K + ⋯ W ⋯ COO − ⋯ interactions. Both pyruvyl and acetyl groups, which are on the periphery of the helix, are involved in the association of the polysaccharide chains and thus appear to be an integral component of the galactoglucan in the nodule invasion process.
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subjects Analytical, structural and metabolic biochemistry
Biological and medical sciences
Carbohydrate Sequence
carbohydrate structure
Carbohydrates
exopolysaccharides
Fundamental and applied biological sciences. Psychology
galactans
glucans
Glucans - chemistry
Heterosides
Models, Molecular
molecular conformation
Molecular Sequence Data
Molecular Structure
mutants
Other biological molecules
Parasitism and symbiosis
Plant physiology and development
polysaccharides
Polysaccharides, Bacterial - chemistry
Sinorhizobium meliloti
Sinorhizobium meliloti - chemistry
structure
Symbiosis
title Molecular architecture of a galactoglucan from Rhizobium meliloti
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