Immunodetection of the Ribosomal Transcription Factor UBF at the Nucleolus Organizer Regions of Fish Cells
A human autoimmune serum to nucleolus organizer regions (NORs) has been used to localize these structures at the light microscopic level in carp and trout tissue culture cells. In interphase cells, the immunofluorescence pattern indicates that the NORs autoantigens are contained exclusively within t...
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| Veröffentlicht in: | Cell Structure and Function 1994, Vol.19(3), pp.153-158 |
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| creator | Rendón, M.C. Bolívar, J. Ortiz, M. Valdivia, M.M. |
| description | A human autoimmune serum to nucleolus organizer regions (NORs) has been used to localize these structures at the light microscopic level in carp and trout tissue culture cells. In interphase cells, the immunofluorescence pattern indicates that the NORs autoantigens are contained exclusively within the nucleolus of carp epithelial (EPC) and trout gonad (RTG) cells. This fluorescence is punctuate rather than uniform, and presumably represents transcriptional complexes of ribosomal DNA. During mitosis, the autoantigens are detected by immunofluorescence microscopy at the chromosomal nucleolus organizer regions of condensed chromosomes, indicating that a considerable quantity of the molecule(s) remains bound to the ribosomal RNA genes. The major nucleolus autoantigen, denned in mammals as the upstream ribosomal binding factor (UBF), has been identified on immunoblots as a 90 kDa protein in extracts from fish cell lines and tissues. Thus, NORs appear to function as nucleation centers for ribosomal RNA together with a complex set of well-conserved protein factors, such as UBF. Our results suggest evolutionary conservation from fish to mammals with respect to ribosomal RNA biosynthesis driven by RNA polymerase I. |
| doi_str_mv | 10.1247/csf.19.153 |
| format | Article |
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In interphase cells, the immunofluorescence pattern indicates that the NORs autoantigens are contained exclusively within the nucleolus of carp epithelial (EPC) and trout gonad (RTG) cells. This fluorescence is punctuate rather than uniform, and presumably represents transcriptional complexes of ribosomal DNA. During mitosis, the autoantigens are detected by immunofluorescence microscopy at the chromosomal nucleolus organizer regions of condensed chromosomes, indicating that a considerable quantity of the molecule(s) remains bound to the ribosomal RNA genes. The major nucleolus autoantigen, denned in mammals as the upstream ribosomal binding factor (UBF), has been identified on immunoblots as a 90 kDa protein in extracts from fish cell lines and tissues. Thus, NORs appear to function as nucleation centers for ribosomal RNA together with a complex set of well-conserved protein factors, such as UBF. Our results suggest evolutionary conservation from fish to mammals with respect to ribosomal RNA biosynthesis driven by RNA polymerase I.</description><identifier>ISSN: 0386-7196</identifier><identifier>EISSN: 1347-3700</identifier><identifier>DOI: 10.1247/csf.19.153</identifier><identifier>PMID: 7954874</identifier><identifier>CODEN: CSFUDY</identifier><language>eng</language><publisher>Kyoto: Japan Society for Cell Biology</publisher><subject>Animals ; Arthritis, Rheumatoid - immunology ; Autoantibodies - immunology ; Autoimmune Diseases - immunology ; Biological and medical sciences ; Carps - metabolism ; Cells, Cultured ; DNA-Binding Proteins - analysis ; Epithelium ; fish ; Fundamental and applied biological sciences. Psychology ; Gonads ; Humans ; Molecular and cellular biology ; Molecular genetics ; nucleolus ; Nucleolus Organizer Region - chemistry ; Nucleolus Organizer Region - immunology ; nucleolus organizer regions ; Perciformes - metabolism ; Phylogeny ; Pol1 Transcription Initiation Complex Proteins ; ribosomal RNA ; Species Specificity ; Transcription Factors - analysis ; Transcription. Transcription factor. Splicing. Rna processing ; Trout - metabolism ; upstream binding factor</subject><ispartof>Cell Structure and Function, 1994, Vol.19(3), pp.153-158</ispartof><rights>Japan Society for Cell Biology</rights><rights>1994 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 1994</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c631t-5fed18936a499998bb53d0214994952de291c7f0d28ec1d19781083ac079e2ce3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1883,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4235150$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7954874$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rendón, M.C.</creatorcontrib><creatorcontrib>Bolívar, J.</creatorcontrib><creatorcontrib>Ortiz, M.</creatorcontrib><creatorcontrib>Valdivia, M.M.</creatorcontrib><title>Immunodetection of the Ribosomal Transcription Factor UBF at the Nucleolus Organizer Regions of Fish Cells</title><title>Cell Structure and Function</title><addtitle>Cell Struct. Funct.</addtitle><description>A human autoimmune serum to nucleolus organizer regions (NORs) has been used to localize these structures at the light microscopic level in carp and trout tissue culture cells. In interphase cells, the immunofluorescence pattern indicates that the NORs autoantigens are contained exclusively within the nucleolus of carp epithelial (EPC) and trout gonad (RTG) cells. This fluorescence is punctuate rather than uniform, and presumably represents transcriptional complexes of ribosomal DNA. During mitosis, the autoantigens are detected by immunofluorescence microscopy at the chromosomal nucleolus organizer regions of condensed chromosomes, indicating that a considerable quantity of the molecule(s) remains bound to the ribosomal RNA genes. The major nucleolus autoantigen, denned in mammals as the upstream ribosomal binding factor (UBF), has been identified on immunoblots as a 90 kDa protein in extracts from fish cell lines and tissues. Thus, NORs appear to function as nucleation centers for ribosomal RNA together with a complex set of well-conserved protein factors, such as UBF. Our results suggest evolutionary conservation from fish to mammals with respect to ribosomal RNA biosynthesis driven by RNA polymerase I.</description><subject>Animals</subject><subject>Arthritis, Rheumatoid - immunology</subject><subject>Autoantibodies - immunology</subject><subject>Autoimmune Diseases - immunology</subject><subject>Biological and medical sciences</subject><subject>Carps - metabolism</subject><subject>Cells, Cultured</subject><subject>DNA-Binding Proteins - analysis</subject><subject>Epithelium</subject><subject>fish</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gonads</subject><subject>Humans</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>nucleolus</subject><subject>Nucleolus Organizer Region - chemistry</subject><subject>Nucleolus Organizer Region - immunology</subject><subject>nucleolus organizer regions</subject><subject>Perciformes - metabolism</subject><subject>Phylogeny</subject><subject>Pol1 Transcription Initiation Complex Proteins</subject><subject>ribosomal RNA</subject><subject>Species Specificity</subject><subject>Transcription Factors - analysis</subject><subject>Transcription. Transcription factor. Splicing. Rna processing</subject><subject>Trout - metabolism</subject><subject>upstream binding factor</subject><issn>0386-7196</issn><issn>1347-3700</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkM9r2zAYhsVY6bJul90HhpUdBs70w7Ks4xqWrVBaKO1ZKPLnRMG2Mn3yYfvrpzQmg-kgId6H95MeQj4wumS8Ul8ddkuml0yKV2TBRKVKoSh9TRZUNHWpmK7fkLeIe0q5pLW6JJdKy6pR1YLsb4dhGkMLCVzyYSxCV6QdFI9-EzAMti-eoh3RRX94idfWpRCL55t1YdMLeT-5HkI_YfEQt3b0fyAWj7DNMB7L1h53xQr6Ht-Ri872CO_n84o8r78_rX6Wdw8_blff7kpXC5ZK2UHLGi1qW-m8ms1GipZylm-VlrwFrplTHW15A461TKuG0UZYR5UG7kBckc-n3kMMvybAZAaPLr_AjhAmNKpWuYrrDH76D9yHKY75bYZVMhuqGy4y9eVEuRgQI3TmEP1g42_DqDnqN1m_Ydpk_Rn-OFdOmwHaMzr7zvn1nFt0tu-yW-fxjFVcSCZpxlYnbI_JbuGc25h8tn2cmP8tj1PFvEnxL93ZaGAUfwHrZKPm</recordid><startdate>1994</startdate><enddate>1994</enddate><creator>Rendón, M.C.</creator><creator>Bolívar, J.</creator><creator>Ortiz, M.</creator><creator>Valdivia, M.M.</creator><general>Japan Society for Cell Biology</general><general>Japan Science and Technology Agency</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>1994</creationdate><title>Immunodetection of the Ribosomal Transcription Factor UBF at the Nucleolus Organizer Regions of Fish Cells</title><author>Rendón, M.C. ; Bolívar, J. ; Ortiz, M. ; Valdivia, M.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c631t-5fed18936a499998bb53d0214994952de291c7f0d28ec1d19781083ac079e2ce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>Arthritis, Rheumatoid - immunology</topic><topic>Autoantibodies - immunology</topic><topic>Autoimmune Diseases - immunology</topic><topic>Biological and medical sciences</topic><topic>Carps - metabolism</topic><topic>Cells, Cultured</topic><topic>DNA-Binding Proteins - analysis</topic><topic>Epithelium</topic><topic>fish</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gonads</topic><topic>Humans</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>nucleolus</topic><topic>Nucleolus Organizer Region - chemistry</topic><topic>Nucleolus Organizer Region - immunology</topic><topic>nucleolus organizer regions</topic><topic>Perciformes - metabolism</topic><topic>Phylogeny</topic><topic>Pol1 Transcription Initiation Complex Proteins</topic><topic>ribosomal RNA</topic><topic>Species Specificity</topic><topic>Transcription Factors - analysis</topic><topic>Transcription. Transcription factor. Splicing. Rna processing</topic><topic>Trout - metabolism</topic><topic>upstream binding factor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rendón, M.C.</creatorcontrib><creatorcontrib>Bolívar, J.</creatorcontrib><creatorcontrib>Ortiz, M.</creatorcontrib><creatorcontrib>Valdivia, M.M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell Structure and Function</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rendón, M.C.</au><au>Bolívar, J.</au><au>Ortiz, M.</au><au>Valdivia, M.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunodetection of the Ribosomal Transcription Factor UBF at the Nucleolus Organizer Regions of Fish Cells</atitle><jtitle>Cell Structure and Function</jtitle><addtitle>Cell Struct. Funct.</addtitle><date>1994</date><risdate>1994</risdate><volume>19</volume><issue>3</issue><spage>153</spage><epage>158</epage><pages>153-158</pages><issn>0386-7196</issn><eissn>1347-3700</eissn><coden>CSFUDY</coden><abstract>A human autoimmune serum to nucleolus organizer regions (NORs) has been used to localize these structures at the light microscopic level in carp and trout tissue culture cells. In interphase cells, the immunofluorescence pattern indicates that the NORs autoantigens are contained exclusively within the nucleolus of carp epithelial (EPC) and trout gonad (RTG) cells. This fluorescence is punctuate rather than uniform, and presumably represents transcriptional complexes of ribosomal DNA. During mitosis, the autoantigens are detected by immunofluorescence microscopy at the chromosomal nucleolus organizer regions of condensed chromosomes, indicating that a considerable quantity of the molecule(s) remains bound to the ribosomal RNA genes. The major nucleolus autoantigen, denned in mammals as the upstream ribosomal binding factor (UBF), has been identified on immunoblots as a 90 kDa protein in extracts from fish cell lines and tissues. Thus, NORs appear to function as nucleation centers for ribosomal RNA together with a complex set of well-conserved protein factors, such as UBF. Our results suggest evolutionary conservation from fish to mammals with respect to ribosomal RNA biosynthesis driven by RNA polymerase I.</abstract><cop>Kyoto</cop><pub>Japan Society for Cell Biology</pub><pmid>7954874</pmid><doi>10.1247/csf.19.153</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese |
| subjects | Animals Arthritis, Rheumatoid - immunology Autoantibodies - immunology Autoimmune Diseases - immunology Biological and medical sciences Carps - metabolism Cells, Cultured DNA-Binding Proteins - analysis Epithelium fish Fundamental and applied biological sciences. Psychology Gonads Humans Molecular and cellular biology Molecular genetics nucleolus Nucleolus Organizer Region - chemistry Nucleolus Organizer Region - immunology nucleolus organizer regions Perciformes - metabolism Phylogeny Pol1 Transcription Initiation Complex Proteins ribosomal RNA Species Specificity Transcription Factors - analysis Transcription. Transcription factor. Splicing. Rna processing Trout - metabolism upstream binding factor |
| title | Immunodetection of the Ribosomal Transcription Factor UBF at the Nucleolus Organizer Regions of Fish Cells |
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