Identification and characterization of acidic hydrolases with chitinase and chitosanase activities from sweet orange callus tissue

Acidic chitinases (EC 3.2.1.14) were isolated and characterized from 4-week-old nonembryogenic Citrus sinensis L. Osbeck cv ‘Valencia’ callus tissue. The enzymes were purified using size exclusion, anion exchange, and chromatofocusing HPLC techniques. Eleven isoforms were isolated with M1s between 2...

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Veröffentlicht in:	Plant and cell physiology 1994-07, Vol.35 (5), p.811-820
Hauptverfasser:	Osswald, W.F. (USDA Agricultural Research Service (USA)), Shapiro, J.P, Doostdar, H, Mcdonald, R.E, Niedz, R.P, Nairn, C.J, Hearn, C.J, Mayer, R.T
Format:	Artikel
Sprache:	eng
Schlagworte:	ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE Agronomy. Soil science and plant productions Amino Acid Sequence Biological and medical sciences Biotechnology Chitinases - genetics Chitinases - isolation & purification CITRUS Citrus - enzymology Citrus - genetics Citrus sinensis CULTIVO DE TEJIDOS CULTURE DE TISSU Defensive proteins Economic plant physiology Enzymes ENZYMIC ACTIVITY Fundamental and applied biological sciences. Psychology Glycoside Hydrolases - genetics Glycoside Hydrolases - isolation & purification HIDROLASAS HYDROLASE HYDROLASES Isoelectric Point LISOZIMA LYSOZYME Metabolism Molecular Sequence Data Molecular Weight Nutrition. Photosynthesis. Respiration. Metabolism Pathogenesis-related proteins PATHOGENICITY Plant physiology and development Plant tissue culture PODER PATOGENO POUVOIR PATHOGENE Sequence Homology, Amino Acid TISSUE CULTURE
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