DNA-Linked RNase H For Site-Selective Cleavage Of RNA

ADNA-linked RNase H (Hybrid Enz-1) (Kanaya et al. (1992) J. Biol. Chem. 267, 8492-8498), in which dGTCATCTCC was attached to E. coli RNase H via a covalent linker of 21 A, was altered to improve the site-specific RNA cleavage by increasing the linker length. The sizes of the linkers on these hybrid...

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Veröffentlicht in:	Bioconjugate chemistry 1994-07, Vol.5 (4), p.327-332
Hauptverfasser:	Uchiyama, Yohtaro, Inoue, Hideo, Ohtsuka, Eiko, Nakai, Chieko, Kanaya, Shigenori, Ueno, Yoshio, Ikehara, Morio
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence DNA - chemistry DNA Restriction Enzymes - metabolism Kinetics Molecular Sequence Data Nucleic Acid Hybridization Ribonuclease H - chemistry Ribonuclease H - metabolism RNA - chemistry RNA - metabolism
Online-Zugang:	Volltext
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