Selective covalent modification of newly synthesized activation-induced sialoglycoconjugates on the T lymphocyte membrane
Resting human lymphocytes were oxidized by periodate and subsequently reduced with borohydride to block the aldehydes formed. The cells were then incubated for 24 or 48 hours with (or without) the mitogenic lectin phytohemagglutinin. A second oxidation with periodate at the indicated times resulted...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1986-03, Vol.135 (2), p.473-479 |
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| creator | Roffman, Ehud Wilchek, Meir |
| description | Resting human lymphocytes were oxidized by periodate and subsequently reduced with borohydride to block the aldehydes formed. The cells were then incubated for 24 or 48 hours with (or without) the mitogenic lectin phytohemagglutinin. A second oxidation with periodate at the indicated times resulted in strong surface-aldehyde formation in samples incubated with the mitogen, compared to control samples which exhibited very low quantities of aldehydes. The data show that this elevation in aldehyde formation was strictly dependent on protein synthesis, similarly to the appearance of Tac antigen in these cells. Cell surface aldehydes were detected in flow cytometry with a fluoresceinated hydrazide molecule and electrophoretically with biocytin hydrazide in conjunction with
125I-streptavidin. The proposed method for the elimination of the chemical reactivity of carbohydrates from the surface of resting lymphocytes thus enabled the selective covalent modification of newly synthesized sialoglycoconjugates formed upon a mitogenic trigger. The data also suggest the existence of a very low turnover rate of sialoglycoconjugates on the resting lymphocyte membrane. |
| doi_str_mv | 10.1016/0006-291X(86)90018-5 |
| format | Article |
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125I-streptavidin. The proposed method for the elimination of the chemical reactivity of carbohydrates from the surface of resting lymphocytes thus enabled the selective covalent modification of newly synthesized sialoglycoconjugates formed upon a mitogenic trigger. The data also suggest the existence of a very low turnover rate of sialoglycoconjugates on the resting lymphocyte membrane.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/0006-291X(86)90018-5</identifier><identifier>PMID: 3008722</identifier><identifier>CODEN: BBRCA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Aldehydes - biosynthesis ; Aldehydes - blood ; Antigens, Surface - analysis ; Applied sciences ; Borohydrides ; Cell Membrane - metabolism ; Electrophoresis, Polyacrylamide Gel ; Exact sciences and technology ; Flow Cytometry ; Humans ; Lymphocyte Activation - drug effects ; lymphocytes T ; man ; membranes ; Other techniques and industries ; Oxidation-Reduction ; Periodic Acid ; Phytohemagglutinins - pharmacology ; Sialic Acids - blood ; sialoglycoconjugates ; Sialoglycoproteins - biosynthesis ; Sialoglycoproteins - blood ; Spectrometry, Fluorescence ; T-Lymphocytes - metabolism</subject><ispartof>Biochemical and biophysical research communications, 1986-03, Vol.135 (2), p.473-479</ispartof><rights>1986</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c332t-9b0445f0ec8fc0e38ee18cef8bb2a0fc5c7ddeb9881398a2d8f7b41344b7da343</citedby><cites>FETCH-LOGICAL-c332t-9b0445f0ec8fc0e38ee18cef8bb2a0fc5c7ddeb9881398a2d8f7b41344b7da343</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0006291X86900185$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8105742$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3008722$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Roffman, Ehud</creatorcontrib><creatorcontrib>Wilchek, Meir</creatorcontrib><title>Selective covalent modification of newly synthesized activation-induced sialoglycoconjugates on the T lymphocyte membrane</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Resting human lymphocytes were oxidized by periodate and subsequently reduced with borohydride to block the aldehydes formed. The cells were then incubated for 24 or 48 hours with (or without) the mitogenic lectin phytohemagglutinin. A second oxidation with periodate at the indicated times resulted in strong surface-aldehyde formation in samples incubated with the mitogen, compared to control samples which exhibited very low quantities of aldehydes. The data show that this elevation in aldehyde formation was strictly dependent on protein synthesis, similarly to the appearance of Tac antigen in these cells. Cell surface aldehydes were detected in flow cytometry with a fluoresceinated hydrazide molecule and electrophoretically with biocytin hydrazide in conjunction with
125I-streptavidin. The proposed method for the elimination of the chemical reactivity of carbohydrates from the surface of resting lymphocytes thus enabled the selective covalent modification of newly synthesized sialoglycoconjugates formed upon a mitogenic trigger. The data also suggest the existence of a very low turnover rate of sialoglycoconjugates on the resting lymphocyte membrane.</description><subject>Aldehydes - biosynthesis</subject><subject>Aldehydes - blood</subject><subject>Antigens, Surface - analysis</subject><subject>Applied sciences</subject><subject>Borohydrides</subject><subject>Cell Membrane - metabolism</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Exact sciences and technology</subject><subject>Flow Cytometry</subject><subject>Humans</subject><subject>Lymphocyte Activation - drug effects</subject><subject>lymphocytes T</subject><subject>man</subject><subject>membranes</subject><subject>Other techniques and industries</subject><subject>Oxidation-Reduction</subject><subject>Periodic Acid</subject><subject>Phytohemagglutinins - pharmacology</subject><subject>Sialic Acids - blood</subject><subject>sialoglycoconjugates</subject><subject>Sialoglycoproteins - biosynthesis</subject><subject>Sialoglycoproteins - blood</subject><subject>Spectrometry, Fluorescence</subject><subject>T-Lymphocytes - metabolism</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2L1TAUxYMo43P0P1DIQkQX1Zs0bdONIINfMODCEdyF9PZmJkPaPJv2Sf3rzfvgLXUVuOd3bi7nMPZcwFsBon4HAHUhW_Hzta7ftABCF9UDthHQQiEFqIdsc0Yesycp3WdGqLq9YBclgG6k3LD1OwXC2e-IY9zZQOPMh9h759HOPo48Oj7S77DytI7zHSX_h3pu946DXvixXzCPkrch3oYVI8bxfrm1MyWe_dnDb3hYh-1dxHUmPtDQTXakp-yRsyHRs9N7yX58-nhz9aW4_vb569WH6wLLUs5F24FSlQNC7RCo1ERCIzndddKCwwqbvqeu1VqUrbay167plCiV6prelqq8ZK-Oe7dT_LVQms3gE1II-Ya4JNPUTSt0Lf4LCtWArJTMoDqCOMWUJnJmO_nBTqsRYPbVmH3uZp-70bU5VGOqbHtx2r90A_Vn06mLrL886TahDS6HhD6dMS2gag6_vz9ilEPbeZpMQk9j7sBPuUnTR__vO_4CJDWuPw</recordid><startdate>19860313</startdate><enddate>19860313</enddate><creator>Roffman, Ehud</creator><creator>Wilchek, Meir</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19860313</creationdate><title>Selective covalent modification of newly synthesized activation-induced sialoglycoconjugates on the T lymphocyte membrane</title><author>Roffman, Ehud ; Wilchek, Meir</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c332t-9b0445f0ec8fc0e38ee18cef8bb2a0fc5c7ddeb9881398a2d8f7b41344b7da343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Aldehydes - biosynthesis</topic><topic>Aldehydes - blood</topic><topic>Antigens, Surface - analysis</topic><topic>Applied sciences</topic><topic>Borohydrides</topic><topic>Cell Membrane - metabolism</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Exact sciences and technology</topic><topic>Flow Cytometry</topic><topic>Humans</topic><topic>Lymphocyte Activation - drug effects</topic><topic>lymphocytes T</topic><topic>man</topic><topic>membranes</topic><topic>Other techniques and industries</topic><topic>Oxidation-Reduction</topic><topic>Periodic Acid</topic><topic>Phytohemagglutinins - pharmacology</topic><topic>Sialic Acids - blood</topic><topic>sialoglycoconjugates</topic><topic>Sialoglycoproteins - biosynthesis</topic><topic>Sialoglycoproteins - blood</topic><topic>Spectrometry, Fluorescence</topic><topic>T-Lymphocytes - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Roffman, Ehud</creatorcontrib><creatorcontrib>Wilchek, Meir</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Roffman, Ehud</au><au>Wilchek, Meir</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Selective covalent modification of newly synthesized activation-induced sialoglycoconjugates on the T lymphocyte membrane</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1986-03-13</date><risdate>1986</risdate><volume>135</volume><issue>2</issue><spage>473</spage><epage>479</epage><pages>473-479</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>Resting human lymphocytes were oxidized by periodate and subsequently reduced with borohydride to block the aldehydes formed. The cells were then incubated for 24 or 48 hours with (or without) the mitogenic lectin phytohemagglutinin. A second oxidation with periodate at the indicated times resulted in strong surface-aldehyde formation in samples incubated with the mitogen, compared to control samples which exhibited very low quantities of aldehydes. The data show that this elevation in aldehyde formation was strictly dependent on protein synthesis, similarly to the appearance of Tac antigen in these cells. Cell surface aldehydes were detected in flow cytometry with a fluoresceinated hydrazide molecule and electrophoretically with biocytin hydrazide in conjunction with
125I-streptavidin. The proposed method for the elimination of the chemical reactivity of carbohydrates from the surface of resting lymphocytes thus enabled the selective covalent modification of newly synthesized sialoglycoconjugates formed upon a mitogenic trigger. The data also suggest the existence of a very low turnover rate of sialoglycoconjugates on the resting lymphocyte membrane.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>3008722</pmid><doi>10.1016/0006-291X(86)90018-5</doi><tpages>7</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1986-03, Vol.135 (2), p.473-479 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Aldehydes - biosynthesis Aldehydes - blood Antigens, Surface - analysis Applied sciences Borohydrides Cell Membrane - metabolism Electrophoresis, Polyacrylamide Gel Exact sciences and technology Flow Cytometry Humans Lymphocyte Activation - drug effects lymphocytes T man membranes Other techniques and industries Oxidation-Reduction Periodic Acid Phytohemagglutinins - pharmacology Sialic Acids - blood sialoglycoconjugates Sialoglycoproteins - biosynthesis Sialoglycoproteins - blood Spectrometry, Fluorescence T-Lymphocytes - metabolism |
| title | Selective covalent modification of newly synthesized activation-induced sialoglycoconjugates on the T lymphocyte membrane |
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