Kinetic and Equilibrium .alpha.-Secondary Tritium Isotope Effects on Reactions Catalyzed by dCMP Hydroxymethylase from Bacteriophage T4

Deoxycytidylate (dCMP) hydroxymethylase (CH) catalyzes the formation of 5-(hydroxymethyl)-dCMP, essential for DNA synthesis in phage T4, from dCMP and methylenetetrahydrofolate (CH2THF). The nucleotide analog 5-fluorodeoxuridylate (FdUMP) stoichiometrically inactivates CH by formation of a covalent...

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Veröffentlicht in:	Biochemistry (Easton) 1994-11, Vol.33 (44), p.13049-13056
Hauptverfasser:	Graves, Karen L, Hardy, Larry W
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriophage T4 - enzymology Catalysis Electrophoresis, Polyacrylamide Gel Escherichia coli - enzymology Fluorodeoxyuridylate - chemistry Fluorodeoxyuridylate - metabolism Hydroxymethyl and Formyl Transferases Kinetics Mutation - genetics Protein Binding - genetics Transferases - chemistry Transferases - genetics Transferases - metabolism Tritium - metabolism
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