Demonstration of the target molecule of a protective IgE antibody in secretory glands of Schistosoma japonicum larvae
We have demonstrated that a mouse monocional IgE antibody, SJ18ε, recognizes a 97 kDa surface molecule (Sj97) of Schistosoma japonlcum larvae and that the antibody Induces partial but significant protection against the skin to lung-stage of S. japonicum infection. The antibody stimulates eosinophil-...
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| Veröffentlicht in: | International immunology 1994-07, Vol.6 (7), p.963-971 |
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| creator | Nara, Takeshi Matsumoto, Naoki Janecharut, Tuenta Matsuda, Hajime Yamamoto, Kazuo Irimura, Tatsuro Nakamura, Kei-ichiro Alkawa, Masamichi Oswald, Isabelle Sher, Alan Kita, Kiyoshi Kojima, Somei |
| description | We have demonstrated that a mouse monocional IgE antibody, SJ18ε, recognizes a 97 kDa surface molecule (Sj97) of Schistosoma japonlcum larvae and that the antibody Induces partial but significant protection against the skin to lung-stage of S. japonicum infection. The antibody stimulates eosinophil- and macrophage-mediated killing of schistosomuia In vitro. In the present study, we isolated the putative full-length cDNA of Sj97 by screening a γgt11 cDNA library from S. japonicum adult worms with SJ18ε.1. The predicted amino add sequence of the cDNA showed highly significant homology to that of S. mansoni paramyosin, a potential vaccine candidate for schistosomiasis. The deletion mutants of S. japonicum paramyosin were expressed inEscherichia coli and the translation product of 443 amino acid residues of paramyosin was found to be recognized by the antibody. Moreover, we observed by Immunoelectron microscopy the presence of paramyosin in the postacetabular. gland as well as In the tegument and muscle layers of the larvae. These results suggest that paramyosin is a secretory protein which may be incorporated into the tegument during the development of schistosomuia, thus becoming a target for protective immunity during the migratory phase of the parasite. |
| doi_str_mv | 10.1093/intimm/6.7.963 |
| format | Article |
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The antibody stimulates eosinophil- and macrophage-mediated killing of schistosomuia In vitro. In the present study, we isolated the putative full-length cDNA of Sj97 by screening a γgt11 cDNA library from S. japonicum adult worms with SJ18ε.1. The predicted amino add sequence of the cDNA showed highly significant homology to that of S. mansoni paramyosin, a potential vaccine candidate for schistosomiasis. The deletion mutants of S. japonicum paramyosin were expressed inEscherichia coli and the translation product of 443 amino acid residues of paramyosin was found to be recognized by the antibody. Moreover, we observed by Immunoelectron microscopy the presence of paramyosin in the postacetabular. gland as well as In the tegument and muscle layers of the larvae. These results suggest that paramyosin is a secretory protein which may be incorporated into the tegument during the development of schistosomuia, thus becoming a target for protective immunity during the migratory phase of the parasite.</description><identifier>ISSN: 0953-8178</identifier><identifier>EISSN: 1460-2377</identifier><identifier>DOI: 10.1093/intimm/6.7.963</identifier><identifier>PMID: 7947464</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Acetabulum ; Amino Acid Sequence ; Amino acids ; Animals ; Antibodies ; Antibodies, Helminth - immunology ; Antibodies, Monoclonal - immunology ; bacterial expression ; Base Sequence ; Biological and medical sciences ; cDNA cloning ; Cloning, Molecular ; Deletion mutant ; DNA, Helminth - analysis ; E coli ; Endocrine Glands - immunology ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Homology ; Immunoelectron microscopy ; Immunoglobulin E ; Immunoglobulin E - immunology ; Larvae ; Leukocytes (eosinophilic) ; Macrophages ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Muscles ; paramyosin ; Parasites ; Schistosoma japonicum ; Schistosoma japonicum - genetics ; Schistosoma japonicum - immunology ; Schistosoma japonicum - ultrastructure ; Schistosoma mansoni - genetics ; Schistosomiasis ; Schistosomiasis japonica - prevention & control ; Sequence Homology, Amino Acid ; Tegument ; Tropical diseases ; Tropomyosin - genetics ; Tropomyosin - immunology ; vaccine development ; Vaccines</subject><ispartof>International immunology, 1994-07, Vol.6 (7), p.963-971</ispartof><rights>1995 INIST-CNRS</rights><rights>Copyright Oxford University Press(England) Jul 1994</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c541t-fabd097c1e244a20fd5f621d73d0825a834f814d3c5f84f63a049c699dcb34f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3316750$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7947464$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nara, Takeshi</creatorcontrib><creatorcontrib>Matsumoto, Naoki</creatorcontrib><creatorcontrib>Janecharut, Tuenta</creatorcontrib><creatorcontrib>Matsuda, Hajime</creatorcontrib><creatorcontrib>Yamamoto, Kazuo</creatorcontrib><creatorcontrib>Irimura, Tatsuro</creatorcontrib><creatorcontrib>Nakamura, Kei-ichiro</creatorcontrib><creatorcontrib>Alkawa, Masamichi</creatorcontrib><creatorcontrib>Oswald, Isabelle</creatorcontrib><creatorcontrib>Sher, Alan</creatorcontrib><creatorcontrib>Kita, Kiyoshi</creatorcontrib><creatorcontrib>Kojima, Somei</creatorcontrib><title>Demonstration of the target molecule of a protective IgE antibody in secretory glands of Schistosoma japonicum larvae</title><title>International immunology</title><addtitle>Int Immunol</addtitle><description>We have demonstrated that a mouse monocional IgE antibody, SJ18ε, recognizes a 97 kDa surface molecule (Sj97) of Schistosoma japonlcum larvae and that the antibody Induces partial but significant protection against the skin to lung-stage of S. japonicum infection. The antibody stimulates eosinophil- and macrophage-mediated killing of schistosomuia In vitro. In the present study, we isolated the putative full-length cDNA of Sj97 by screening a γgt11 cDNA library from S. japonicum adult worms with SJ18ε.1. The predicted amino add sequence of the cDNA showed highly significant homology to that of S. mansoni paramyosin, a potential vaccine candidate for schistosomiasis. The deletion mutants of S. japonicum paramyosin were expressed inEscherichia coli and the translation product of 443 amino acid residues of paramyosin was found to be recognized by the antibody. Moreover, we observed by Immunoelectron microscopy the presence of paramyosin in the postacetabular. gland as well as In the tegument and muscle layers of the larvae. These results suggest that paramyosin is a secretory protein which may be incorporated into the tegument during the development of schistosomuia, thus becoming a target for protective immunity during the migratory phase of the parasite.</description><subject>Acetabulum</subject><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies, Helminth - immunology</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>bacterial expression</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>cDNA cloning</subject><subject>Cloning, Molecular</subject><subject>Deletion mutant</subject><subject>DNA, Helminth - analysis</subject><subject>E coli</subject><subject>Endocrine Glands - immunology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Homology</subject><subject>Immunoelectron microscopy</subject><subject>Immunoglobulin E</subject><subject>Immunoglobulin E - immunology</subject><subject>Larvae</subject><subject>Leukocytes (eosinophilic)</subject><subject>Macrophages</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Muscles</subject><subject>paramyosin</subject><subject>Parasites</subject><subject>Schistosoma japonicum</subject><subject>Schistosoma japonicum - genetics</subject><subject>Schistosoma japonicum - immunology</subject><subject>Schistosoma japonicum - ultrastructure</subject><subject>Schistosoma mansoni - genetics</subject><subject>Schistosomiasis</subject><subject>Schistosomiasis japonica - prevention & control</subject><subject>Sequence Homology, Amino Acid</subject><subject>Tegument</subject><subject>Tropical diseases</subject><subject>Tropomyosin - genetics</subject><subject>Tropomyosin - immunology</subject><subject>vaccine development</subject><subject>Vaccines</subject><issn>0953-8178</issn><issn>1460-2377</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtvEzEURi0EKqGwZYdkIcRuUnv8XkLpS6pUlWZRsbEcj506zIyD7anIv8dRoizYdGXJ37nXn3UA-IjRHCNFzsJYwjCc8bmYK05egRmmHDUtEeI1mCHFSCOxkG_Bu5zXCCHSKnICToSignI6A9MPN8Qxl2RKiCOMHpYnB4tJK1fgEHtnp97trg3cpFicLeHZwZvVBTT14WXstjCMMDubXIlpC1e9Gbu8G3iwTyGXmONg4Nps4hjsNMDepGfj3oM33vTZfTicp2BxebE4v25u765uzr_dNpZRXBpvlh1SwmLXUmpa5DvmeYs7QTokW2YkoV5i2hHLvKSeE4Ooslypzi5rRE7B1_3aWv3P5HLRQ8jW9bWji1PWggvJuGAvgi1iAjNJXgQxF4gyKiv4-T9wHac01s9qrBiq9bmq0HwP2RRzTs7rTQqDSVuNkd7Z1Xu7mmuhq9068OmwdVoOrjviB501_3LITbam98mMNuQjRkjtx1DFmj1WBbm_x9ik35oLIpi-fvylf4rL73xxda8R-QdJZr5P</recordid><startdate>19940701</startdate><enddate>19940701</enddate><creator>Nara, Takeshi</creator><creator>Matsumoto, Naoki</creator><creator>Janecharut, Tuenta</creator><creator>Matsuda, Hajime</creator><creator>Yamamoto, Kazuo</creator><creator>Irimura, Tatsuro</creator><creator>Nakamura, Kei-ichiro</creator><creator>Alkawa, Masamichi</creator><creator>Oswald, Isabelle</creator><creator>Sher, Alan</creator><creator>Kita, Kiyoshi</creator><creator>Kojima, Somei</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19940701</creationdate><title>Demonstration of the target molecule of a protective IgE antibody in secretory glands of Schistosoma japonicum larvae</title><author>Nara, Takeshi ; Matsumoto, Naoki ; Janecharut, Tuenta ; Matsuda, Hajime ; Yamamoto, Kazuo ; Irimura, Tatsuro ; Nakamura, Kei-ichiro ; Alkawa, Masamichi ; Oswald, Isabelle ; Sher, Alan ; Kita, Kiyoshi ; Kojima, Somei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c541t-fabd097c1e244a20fd5f621d73d0825a834f814d3c5f84f63a049c699dcb34f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Acetabulum</topic><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Antibodies, Helminth - immunology</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>bacterial expression</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>cDNA cloning</topic><topic>Cloning, Molecular</topic><topic>Deletion mutant</topic><topic>DNA, Helminth - analysis</topic><topic>E coli</topic><topic>Endocrine Glands - immunology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Homology</topic><topic>Immunoelectron microscopy</topic><topic>Immunoglobulin E</topic><topic>Immunoglobulin E - immunology</topic><topic>Larvae</topic><topic>Leukocytes (eosinophilic)</topic><topic>Macrophages</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Muscles</topic><topic>paramyosin</topic><topic>Parasites</topic><topic>Schistosoma japonicum</topic><topic>Schistosoma japonicum - genetics</topic><topic>Schistosoma japonicum - immunology</topic><topic>Schistosoma japonicum - ultrastructure</topic><topic>Schistosoma mansoni - genetics</topic><topic>Schistosomiasis</topic><topic>Schistosomiasis japonica - prevention & control</topic><topic>Sequence Homology, Amino Acid</topic><topic>Tegument</topic><topic>Tropical diseases</topic><topic>Tropomyosin - genetics</topic><topic>Tropomyosin - immunology</topic><topic>vaccine development</topic><topic>Vaccines</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nara, Takeshi</creatorcontrib><creatorcontrib>Matsumoto, Naoki</creatorcontrib><creatorcontrib>Janecharut, Tuenta</creatorcontrib><creatorcontrib>Matsuda, Hajime</creatorcontrib><creatorcontrib>Yamamoto, Kazuo</creatorcontrib><creatorcontrib>Irimura, Tatsuro</creatorcontrib><creatorcontrib>Nakamura, Kei-ichiro</creatorcontrib><creatorcontrib>Alkawa, Masamichi</creatorcontrib><creatorcontrib>Oswald, Isabelle</creatorcontrib><creatorcontrib>Sher, Alan</creatorcontrib><creatorcontrib>Kita, Kiyoshi</creatorcontrib><creatorcontrib>Kojima, Somei</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>International immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nara, Takeshi</au><au>Matsumoto, Naoki</au><au>Janecharut, Tuenta</au><au>Matsuda, Hajime</au><au>Yamamoto, Kazuo</au><au>Irimura, Tatsuro</au><au>Nakamura, Kei-ichiro</au><au>Alkawa, Masamichi</au><au>Oswald, Isabelle</au><au>Sher, Alan</au><au>Kita, Kiyoshi</au><au>Kojima, Somei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Demonstration of the target molecule of a protective IgE antibody in secretory glands of Schistosoma japonicum larvae</atitle><jtitle>International immunology</jtitle><addtitle>Int Immunol</addtitle><date>1994-07-01</date><risdate>1994</risdate><volume>6</volume><issue>7</issue><spage>963</spage><epage>971</epage><pages>963-971</pages><issn>0953-8178</issn><eissn>1460-2377</eissn><abstract>We have demonstrated that a mouse monocional IgE antibody, SJ18ε, recognizes a 97 kDa surface molecule (Sj97) of Schistosoma japonlcum larvae and that the antibody Induces partial but significant protection against the skin to lung-stage of S. japonicum infection. The antibody stimulates eosinophil- and macrophage-mediated killing of schistosomuia In vitro. In the present study, we isolated the putative full-length cDNA of Sj97 by screening a γgt11 cDNA library from S. japonicum adult worms with SJ18ε.1. The predicted amino add sequence of the cDNA showed highly significant homology to that of S. mansoni paramyosin, a potential vaccine candidate for schistosomiasis. The deletion mutants of S. japonicum paramyosin were expressed inEscherichia coli and the translation product of 443 amino acid residues of paramyosin was found to be recognized by the antibody. Moreover, we observed by Immunoelectron microscopy the presence of paramyosin in the postacetabular. gland as well as In the tegument and muscle layers of the larvae. These results suggest that paramyosin is a secretory protein which may be incorporated into the tegument during the development of schistosomuia, thus becoming a target for protective immunity during the migratory phase of the parasite.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>7947464</pmid><doi>10.1093/intimm/6.7.963</doi><tpages>9</tpages></addata></record> |
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| ispartof | International immunology, 1994-07, Vol.6 (7), p.963-971 |
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| source | Oxford University Press Journals Digital Archive legacy; MEDLINE |
| subjects | Acetabulum Amino Acid Sequence Amino acids Animals Antibodies Antibodies, Helminth - immunology Antibodies, Monoclonal - immunology bacterial expression Base Sequence Biological and medical sciences cDNA cloning Cloning, Molecular Deletion mutant DNA, Helminth - analysis E coli Endocrine Glands - immunology Fundamental and applied biological sciences. Psychology Gene expression Homology Immunoelectron microscopy Immunoglobulin E Immunoglobulin E - immunology Larvae Leukocytes (eosinophilic) Macrophages Molecular and cellular biology Molecular genetics Molecular Sequence Data Muscles paramyosin Parasites Schistosoma japonicum Schistosoma japonicum - genetics Schistosoma japonicum - immunology Schistosoma japonicum - ultrastructure Schistosoma mansoni - genetics Schistosomiasis Schistosomiasis japonica - prevention & control Sequence Homology, Amino Acid Tegument Tropical diseases Tropomyosin - genetics Tropomyosin - immunology vaccine development Vaccines |
| title | Demonstration of the target molecule of a protective IgE antibody in secretory glands of Schistosoma japonicum larvae |
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