Novel synaptic responses mediated by dopamine and γ-aminobutyric acid in neuroendocrine cells of the intermediate pituitary
Neuroendocrine cells of the pituitary pars intermedia contain pro-opiomelanocortin and secrete mainly α-melanocyte-stimulating hormone and, in lesser amounts, corticotropin and endorphin. We have recorded intracellularly from rat pars intermedia cells in vitro and analysed the synaptic inputs to the...
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| Veröffentlicht in: | Neuroscience letters 1986-02, Vol.64 (1), p.35-40 |
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| creator | MacVicar, B.A. Pittman, Q.J. |
| description | Neuroendocrine cells of the pituitary pars intermedia contain pro-opiomelanocortin and secrete mainly α-melanocyte-stimulating hormone and, in lesser amounts, corticotropin and endorphin. We have recorded intracellularly from rat pars intermedia cells in vitro and analysed the synaptic inputs to these cells. Electrical stimulation of the pituitary stalk evoked a biphasic postsynaptic response in pars intermedia cells. An initial inhibitory postsynaptic potential was most likely mediated by γ-aminobutyric acid (GABA) because there was a conductance increase, and it was blocked by bicuculline, a GABA antagonist. A more prolonged hyperpolarization was also observed which could last 10–30 s and was associated with a decreased whole cell conductance. This late hyperpolarization was blocked by the dopamine antagonists, chlorpromazine and domperidone, and was therefore most likely dopamine-mediated. |
| doi_str_mv | 10.1016/0304-3940(86)90659-2 |
| format | Article |
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We have recorded intracellularly from rat pars intermedia cells in vitro and analysed the synaptic inputs to these cells. Electrical stimulation of the pituitary stalk evoked a biphasic postsynaptic response in pars intermedia cells. An initial inhibitory postsynaptic potential was most likely mediated by γ-aminobutyric acid (GABA) because there was a conductance increase, and it was blocked by bicuculline, a GABA antagonist. A more prolonged hyperpolarization was also observed which could last 10–30 s and was associated with a decreased whole cell conductance. This late hyperpolarization was blocked by the dopamine antagonists, chlorpromazine and domperidone, and was therefore most likely dopamine-mediated.</description><identifier>ISSN: 0304-3940</identifier><identifier>EISSN: 1872-7972</identifier><identifier>DOI: 10.1016/0304-3940(86)90659-2</identifier><identifier>PMID: 3008038</identifier><identifier>CODEN: NELED5</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Animals ; Bicuculline - pharmacology ; Biological and medical sciences ; Chlorpromazine - pharmacology ; Domperidone - pharmacology ; dopamine ; Dopamine - physiology ; dopamine antagonist ; Electric Conductivity ; Fundamental and applied biological sciences. Psychology ; gamma-Aminobutyric Acid - physiology ; Hormones and neuropeptides. Regulation ; Hypothalamus. Hypophysis. Epiphysis. Urophysis ; inhibitory postsynaptic potential ; Membrane Potentials - drug effects ; pars intermedia ; pituitary ; Pituitary Gland, Anterior - physiology ; rat ; Rats ; Rats, Inbred Strains ; Synapses - physiology ; Synaptic Transmission - drug effects ; Vertebrates: endocrinology ; γ-aminobutyric acid</subject><ispartof>Neuroscience letters, 1986-02, Vol.64 (1), p.35-40</ispartof><rights>1986</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-d4168e94e84be17ed83b2f985afd7cd1b6c7bc2b29fca500d1c20748066eb4063</citedby><cites>FETCH-LOGICAL-c386t-d4168e94e84be17ed83b2f985afd7cd1b6c7bc2b29fca500d1c20748066eb4063</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0304394086906592$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7958475$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3008038$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MacVicar, B.A.</creatorcontrib><creatorcontrib>Pittman, Q.J.</creatorcontrib><title>Novel synaptic responses mediated by dopamine and γ-aminobutyric acid in neuroendocrine cells of the intermediate pituitary</title><title>Neuroscience letters</title><addtitle>Neurosci Lett</addtitle><description>Neuroendocrine cells of the pituitary pars intermedia contain pro-opiomelanocortin and secrete mainly α-melanocyte-stimulating hormone and, in lesser amounts, corticotropin and endorphin. We have recorded intracellularly from rat pars intermedia cells in vitro and analysed the synaptic inputs to these cells. Electrical stimulation of the pituitary stalk evoked a biphasic postsynaptic response in pars intermedia cells. An initial inhibitory postsynaptic potential was most likely mediated by γ-aminobutyric acid (GABA) because there was a conductance increase, and it was blocked by bicuculline, a GABA antagonist. A more prolonged hyperpolarization was also observed which could last 10–30 s and was associated with a decreased whole cell conductance. This late hyperpolarization was blocked by the dopamine antagonists, chlorpromazine and domperidone, and was therefore most likely dopamine-mediated.</description><subject>Animals</subject><subject>Bicuculline - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Chlorpromazine - pharmacology</subject><subject>Domperidone - pharmacology</subject><subject>dopamine</subject><subject>Dopamine - physiology</subject><subject>dopamine antagonist</subject><subject>Electric Conductivity</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gamma-Aminobutyric Acid - physiology</subject><subject>Hormones and neuropeptides. Regulation</subject><subject>Hypothalamus. Hypophysis. Epiphysis. Urophysis</subject><subject>inhibitory postsynaptic potential</subject><subject>Membrane Potentials - drug effects</subject><subject>pars intermedia</subject><subject>pituitary</subject><subject>Pituitary Gland, Anterior - physiology</subject><subject>rat</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Synapses - physiology</subject><subject>Synaptic Transmission - drug effects</subject><subject>Vertebrates: endocrinology</subject><subject>γ-aminobutyric acid</subject><issn>0304-3940</issn><issn>1872-7972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM9qFTEUh4Mo9bb6BgpZiNTF1GQmk2Q2BSlqC8VudB3y5wxG5iZjkilc8K18D5_JjHe4S1ch_L5z-J0PoVeUXFFC-XvSEdZ0AyOXkr8bCO-Hpn2CdlSKthGDaJ-i3Ql5js5z_kEI6WnPztBZR4gkndyhX1_iI0w4H4Kei7c4QZ5jyJDxHpzXBRw2B-zirPc-ANbB4T-_m_UTzVIOqY5o6x32AQdYUoTgok0ramGaMo4jLt-hxgXSthHPviy-6HR4gZ6Nesrwcnsv0LdPH7_e3Db3D5_vbj7cN7aTvDSOUS5hYCCZASrAyc604yB7PTphHTXcCmNb0w6j1T0hjtqWCCYJ52AY4d0FenvcO6f4c4Fc1N7ntZ8OEJesBBei5ZxVkB1Bm2LOCUY1J7-vTRUlapWuVqNqNaokV_-kq7aOvd72L6YeeRraLNf8zZbrbPU0Jh2szydMDL1koq_Y9RGD6uLRQ1LZegi2aktgi3LR_7_HX7JeoSA</recordid><startdate>19860214</startdate><enddate>19860214</enddate><creator>MacVicar, B.A.</creator><creator>Pittman, Q.J.</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19860214</creationdate><title>Novel synaptic responses mediated by dopamine and γ-aminobutyric acid in neuroendocrine cells of the intermediate pituitary</title><author>MacVicar, B.A. ; Pittman, Q.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-d4168e94e84be17ed83b2f985afd7cd1b6c7bc2b29fca500d1c20748066eb4063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Animals</topic><topic>Bicuculline - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Chlorpromazine - pharmacology</topic><topic>Domperidone - pharmacology</topic><topic>dopamine</topic><topic>Dopamine - physiology</topic><topic>dopamine antagonist</topic><topic>Electric Conductivity</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gamma-Aminobutyric Acid - physiology</topic><topic>Hormones and neuropeptides. Regulation</topic><topic>Hypothalamus. Hypophysis. Epiphysis. Urophysis</topic><topic>inhibitory postsynaptic potential</topic><topic>Membrane Potentials - drug effects</topic><topic>pars intermedia</topic><topic>pituitary</topic><topic>Pituitary Gland, Anterior - physiology</topic><topic>rat</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Synapses - physiology</topic><topic>Synaptic Transmission - drug effects</topic><topic>Vertebrates: endocrinology</topic><topic>γ-aminobutyric acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MacVicar, B.A.</creatorcontrib><creatorcontrib>Pittman, Q.J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MacVicar, B.A.</au><au>Pittman, Q.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Novel synaptic responses mediated by dopamine and γ-aminobutyric acid in neuroendocrine cells of the intermediate pituitary</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>1986-02-14</date><risdate>1986</risdate><volume>64</volume><issue>1</issue><spage>35</spage><epage>40</epage><pages>35-40</pages><issn>0304-3940</issn><eissn>1872-7972</eissn><coden>NELED5</coden><abstract>Neuroendocrine cells of the pituitary pars intermedia contain pro-opiomelanocortin and secrete mainly α-melanocyte-stimulating hormone and, in lesser amounts, corticotropin and endorphin. We have recorded intracellularly from rat pars intermedia cells in vitro and analysed the synaptic inputs to these cells. Electrical stimulation of the pituitary stalk evoked a biphasic postsynaptic response in pars intermedia cells. An initial inhibitory postsynaptic potential was most likely mediated by γ-aminobutyric acid (GABA) because there was a conductance increase, and it was blocked by bicuculline, a GABA antagonist. A more prolonged hyperpolarization was also observed which could last 10–30 s and was associated with a decreased whole cell conductance. This late hyperpolarization was blocked by the dopamine antagonists, chlorpromazine and domperidone, and was therefore most likely dopamine-mediated.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>3008038</pmid><doi>10.1016/0304-3940(86)90659-2</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Neuroscience letters, 1986-02, Vol.64 (1), p.35-40 |
| issn | 0304-3940 1872-7972 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Bicuculline - pharmacology Biological and medical sciences Chlorpromazine - pharmacology Domperidone - pharmacology dopamine Dopamine - physiology dopamine antagonist Electric Conductivity Fundamental and applied biological sciences. Psychology gamma-Aminobutyric Acid - physiology Hormones and neuropeptides. Regulation Hypothalamus. Hypophysis. Epiphysis. Urophysis inhibitory postsynaptic potential Membrane Potentials - drug effects pars intermedia pituitary Pituitary Gland, Anterior - physiology rat Rats Rats, Inbred Strains Synapses - physiology Synaptic Transmission - drug effects Vertebrates: endocrinology γ-aminobutyric acid |
| title | Novel synaptic responses mediated by dopamine and γ-aminobutyric acid in neuroendocrine cells of the intermediate pituitary |
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