Characterization of cDNAs Species Encoding the Tat Protein of Caprine Arthritis Encephalitis Virus
Two distinct species of caprine arthritis encephalitis virus (CAEV) tat cDNAs were isolated early after infection of a Himalayan tahr cell line. Sequence analyses predicted that one cDNA (pCEV/e1) represented a polycistronic transcript that encodes Tat and Rev as well as an N-terminally truncated tr...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 1994-11, Vol.204 (2), p.828-834 |
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| container_title | Virology (New York, N.Y.) |
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| creator | Kalinski, Hagar Mashiah, Pnina Rotem, Dagan Orzech, Yael Sherman, Levana Miki, Toru Yaniv, Abraham Gazit, Arnona Tronick, Steven R. |
| description | Two distinct species of caprine arthritis encephalitis virus (CAEV) tat cDNAs were isolated early after infection of a Himalayan tahr cell line. Sequence analyses predicted that one cDNA (pCEV/e1) represented a polycistronic transcript that encodes Tat and Rev as well as an N-terminally truncated transmembrane protein and a protein, designated X, whose function is unknown; whereas the other cDNA (pCEV/f1) encodes Tat and the env gene products pCEV/e1 trans-activated a CAEV LTR-chloramphenicol acetyltransferase reporter gene in goat synovial membrane cells. This activity was shown to be encoded by the Tat open reading frame by analysis of a deletion mutant. Because the pCAEV/f1 insert was unstable in plasmid form, its Tat activity could not be convincingly demonstrated. The target sequences for Tat within the CAEV LTR were localized to the U3 region which, when placed in either orientation upstream of heterologous promoters, was able to confer responsiveness to Tat. |
| doi_str_mv | 10.1006/viro.1994.1602 |
| format | Article |
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Sequence analyses predicted that one cDNA (pCEV/e1) represented a polycistronic transcript that encodes Tat and Rev as well as an N-terminally truncated transmembrane protein and a protein, designated X, whose function is unknown; whereas the other cDNA (pCEV/f1) encodes Tat and the env gene products pCEV/e1 trans-activated a CAEV LTR-chloramphenicol acetyltransferase reporter gene in goat synovial membrane cells. This activity was shown to be encoded by the Tat open reading frame by analysis of a deletion mutant. Because the pCAEV/f1 insert was unstable in plasmid form, its Tat activity could not be convincingly demonstrated. The target sequences for Tat within the CAEV LTR were localized to the U3 region which, when placed in either orientation upstream of heterologous promoters, was able to confer responsiveness to Tat.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1006/viro.1994.1602</identifier><identifier>PMID: 7941354</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>ADN ; Amino Acid Sequence ; Animals ; Arthritis-Encephalitis Virus, Caprine - genetics ; Base Sequence ; caprine arthritis encephalitis virus ; Cell Line ; CODE GENETIQUE ; CODIGO GENETICO ; COMPOSICION QUIMICA ; COMPOSITION CHIMIQUE ; DNA, Complementary - chemistry ; DNA, Complementary - isolation & purification ; GENE ; Gene Products, tat - chemistry ; Gene Products, tat - genetics ; GENES ; Genes, Viral ; Molecular Sequence Data ; Open Reading Frames ; PROTEINAS ; PROTEINE ; Repetitive Sequences, Nucleic Acid ; SECUENCIA NUCLEICA ; SEQUENCE NUCLEIQUE ; Transcriptional Activation ; VIRUS ARTHRITE ENCEPHALITE CAPRINE ; VIRUS ARTRITIS ENCEFALITIS CAPRINA ; VIRUS DE LOS ANIMALES ; VIRUS DES ANIMAUX</subject><ispartof>Virology (New York, N.Y.), 1994-11, Vol.204 (2), p.828-834</ispartof><rights>1994 Academic Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-ffdc918f0b2e33672be3eb9b405fd51a239249984fd4a8f9e223f63d506af1013</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0042682284716023$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7941354$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kalinski, Hagar</creatorcontrib><creatorcontrib>Mashiah, Pnina</creatorcontrib><creatorcontrib>Rotem, Dagan</creatorcontrib><creatorcontrib>Orzech, Yael</creatorcontrib><creatorcontrib>Sherman, Levana</creatorcontrib><creatorcontrib>Miki, Toru</creatorcontrib><creatorcontrib>Yaniv, Abraham</creatorcontrib><creatorcontrib>Gazit, Arnona</creatorcontrib><creatorcontrib>Tronick, Steven R.</creatorcontrib><title>Characterization of cDNAs Species Encoding the Tat Protein of Caprine Arthritis Encephalitis Virus</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>Two distinct species of caprine arthritis encephalitis virus (CAEV) tat cDNAs were isolated early after infection of a Himalayan tahr cell line. Sequence analyses predicted that one cDNA (pCEV/e1) represented a polycistronic transcript that encodes Tat and Rev as well as an N-terminally truncated transmembrane protein and a protein, designated X, whose function is unknown; whereas the other cDNA (pCEV/f1) encodes Tat and the env gene products pCEV/e1 trans-activated a CAEV LTR-chloramphenicol acetyltransferase reporter gene in goat synovial membrane cells. This activity was shown to be encoded by the Tat open reading frame by analysis of a deletion mutant. Because the pCAEV/f1 insert was unstable in plasmid form, its Tat activity could not be convincingly demonstrated. The target sequences for Tat within the CAEV LTR were localized to the U3 region which, when placed in either orientation upstream of heterologous promoters, was able to confer responsiveness to Tat.</description><subject>ADN</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Arthritis-Encephalitis Virus, Caprine - genetics</subject><subject>Base Sequence</subject><subject>caprine arthritis encephalitis virus</subject><subject>Cell Line</subject><subject>CODE GENETIQUE</subject><subject>CODIGO GENETICO</subject><subject>COMPOSICION QUIMICA</subject><subject>COMPOSITION CHIMIQUE</subject><subject>DNA, Complementary - chemistry</subject><subject>DNA, Complementary - isolation & purification</subject><subject>GENE</subject><subject>Gene Products, tat - chemistry</subject><subject>Gene Products, tat - genetics</subject><subject>GENES</subject><subject>Genes, Viral</subject><subject>Molecular Sequence Data</subject><subject>Open Reading Frames</subject><subject>PROTEINAS</subject><subject>PROTEINE</subject><subject>Repetitive Sequences, Nucleic Acid</subject><subject>SECUENCIA NUCLEICA</subject><subject>SEQUENCE NUCLEIQUE</subject><subject>Transcriptional Activation</subject><subject>VIRUS ARTHRITE ENCEPHALITE CAPRINE</subject><subject>VIRUS ARTRITIS ENCEFALITIS CAPRINA</subject><subject>VIRUS DE LOS ANIMALES</subject><subject>VIRUS DES ANIMAUX</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1rGzEQhkVpSZ201x4KgT3ltu7oY6XV0ThJEwhtIUmvQqsdxQr2ypXkQPrru2ub3kpPw_A-mhk9hHyiMKcA8stLSHFOtRZzKoG9ITMKWtbABX1LZgCC1bJl7D05zfkZxl4pOCEnSgvKGzEj3XJlk3UFU_htS4hDFX3lLr8tcnW_RRcwV1eDi30YnqqywurBlupHigXDnlzabQoDVotUVimUsKdxu7LrffMzpF3-QN55u8748VjPyOP11cPypr77_vV2ubirHW91qb3vnaath44h51KxDjl2uhPQ-L6hlnHNhNat8L2wrdfIGPeS9w1I6ylQfkYuDnO3Kf7aYS5mE7LD9doOGHfZKKmkVtD-F6RSMkVBjeD8ALoUc07ozfjbjU2vhoKZ7JvJvpnsm8n--OD8OHnXbbD_ix91j_nnQ-5tNPYphWwe73XDBWum-9tDiKOkl4DJ5NH_qLMPCV0xfQz_2vsHGXKcHQ</recordid><startdate>19941101</startdate><enddate>19941101</enddate><creator>Kalinski, Hagar</creator><creator>Mashiah, Pnina</creator><creator>Rotem, Dagan</creator><creator>Orzech, Yael</creator><creator>Sherman, Levana</creator><creator>Miki, Toru</creator><creator>Yaniv, Abraham</creator><creator>Gazit, Arnona</creator><creator>Tronick, Steven R.</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19941101</creationdate><title>Characterization of cDNAs Species Encoding the Tat Protein of Caprine Arthritis Encephalitis Virus</title><author>Kalinski, Hagar ; Mashiah, Pnina ; Rotem, Dagan ; Orzech, Yael ; Sherman, Levana ; Miki, Toru ; Yaniv, Abraham ; Gazit, Arnona ; Tronick, Steven R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-ffdc918f0b2e33672be3eb9b405fd51a239249984fd4a8f9e223f63d506af1013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>ADN</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Arthritis-Encephalitis Virus, Caprine - genetics</topic><topic>Base Sequence</topic><topic>caprine arthritis encephalitis virus</topic><topic>Cell Line</topic><topic>CODE GENETIQUE</topic><topic>CODIGO GENETICO</topic><topic>COMPOSICION QUIMICA</topic><topic>COMPOSITION CHIMIQUE</topic><topic>DNA, Complementary - chemistry</topic><topic>DNA, Complementary - isolation & purification</topic><topic>GENE</topic><topic>Gene Products, tat - chemistry</topic><topic>Gene Products, tat - genetics</topic><topic>GENES</topic><topic>Genes, Viral</topic><topic>Molecular Sequence Data</topic><topic>Open Reading Frames</topic><topic>PROTEINAS</topic><topic>PROTEINE</topic><topic>Repetitive Sequences, Nucleic Acid</topic><topic>SECUENCIA NUCLEICA</topic><topic>SEQUENCE NUCLEIQUE</topic><topic>Transcriptional Activation</topic><topic>VIRUS ARTHRITE ENCEPHALITE CAPRINE</topic><topic>VIRUS ARTRITIS ENCEFALITIS CAPRINA</topic><topic>VIRUS DE LOS ANIMALES</topic><topic>VIRUS DES ANIMAUX</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kalinski, Hagar</creatorcontrib><creatorcontrib>Mashiah, Pnina</creatorcontrib><creatorcontrib>Rotem, Dagan</creatorcontrib><creatorcontrib>Orzech, Yael</creatorcontrib><creatorcontrib>Sherman, Levana</creatorcontrib><creatorcontrib>Miki, Toru</creatorcontrib><creatorcontrib>Yaniv, Abraham</creatorcontrib><creatorcontrib>Gazit, Arnona</creatorcontrib><creatorcontrib>Tronick, Steven R.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kalinski, Hagar</au><au>Mashiah, Pnina</au><au>Rotem, Dagan</au><au>Orzech, Yael</au><au>Sherman, Levana</au><au>Miki, Toru</au><au>Yaniv, Abraham</au><au>Gazit, Arnona</au><au>Tronick, Steven R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of cDNAs Species Encoding the Tat Protein of Caprine Arthritis Encephalitis Virus</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>1994-11-01</date><risdate>1994</risdate><volume>204</volume><issue>2</issue><spage>828</spage><epage>834</epage><pages>828-834</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><abstract>Two distinct species of caprine arthritis encephalitis virus (CAEV) tat cDNAs were isolated early after infection of a Himalayan tahr cell line. Sequence analyses predicted that one cDNA (pCEV/e1) represented a polycistronic transcript that encodes Tat and Rev as well as an N-terminally truncated transmembrane protein and a protein, designated X, whose function is unknown; whereas the other cDNA (pCEV/f1) encodes Tat and the env gene products pCEV/e1 trans-activated a CAEV LTR-chloramphenicol acetyltransferase reporter gene in goat synovial membrane cells. This activity was shown to be encoded by the Tat open reading frame by analysis of a deletion mutant. Because the pCAEV/f1 insert was unstable in plasmid form, its Tat activity could not be convincingly demonstrated. The target sequences for Tat within the CAEV LTR were localized to the U3 region which, when placed in either orientation upstream of heterologous promoters, was able to confer responsiveness to Tat.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>7941354</pmid><doi>10.1006/viro.1994.1602</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0042-6822 |
| ispartof | Virology (New York, N.Y.), 1994-11, Vol.204 (2), p.828-834 |
| issn | 0042-6822 1096-0341 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals; EZB-FREE-00999 freely available EZB journals |
| subjects | ADN Amino Acid Sequence Animals Arthritis-Encephalitis Virus, Caprine - genetics Base Sequence caprine arthritis encephalitis virus Cell Line CODE GENETIQUE CODIGO GENETICO COMPOSICION QUIMICA COMPOSITION CHIMIQUE DNA, Complementary - chemistry DNA, Complementary - isolation & purification GENE Gene Products, tat - chemistry Gene Products, tat - genetics GENES Genes, Viral Molecular Sequence Data Open Reading Frames PROTEINAS PROTEINE Repetitive Sequences, Nucleic Acid SECUENCIA NUCLEICA SEQUENCE NUCLEIQUE Transcriptional Activation VIRUS ARTHRITE ENCEPHALITE CAPRINE VIRUS ARTRITIS ENCEFALITIS CAPRINA VIRUS DE LOS ANIMALES VIRUS DES ANIMAUX |
| title | Characterization of cDNAs Species Encoding the Tat Protein of Caprine Arthritis Encephalitis Virus |
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