3H-imipramine binding in aged mouse brain: regulation by ions and serotonin
The density of binding sites (Bmax) for 3H-imipramine was elevated in cerebral cortical, hypothalamic and hippocampal membranes from 24 month old male C57BL/6J mice. Cerebellar binding was constant with increasing age. There were no changes in the equilibrium dissociation constant (Kd) for 3H-imipra...
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| Veröffentlicht in: | Neurobiology of aging 1986-03, Vol.7 (2), p.83-87 |
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| container_title | Neurobiology of aging |
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| creator | Severson, James A. |
| description | The density of binding sites (Bmax) for
3H-imipramine was elevated in cerebral cortical, hypothalamic and hippocampal membranes from 24 month old male C57BL/6J mice. Cerebellar binding was constant with increasing age. There were no changes in the equilibrium dissociation constant (Kd) for
3H-imipramine in any brain region. The increase in the binding of
3H-imipramine induced by sodium and chloride ions
in vitro was diminished in cerebral cortical homogenates from aged mice; both the sodium-sensitive and chloride-sensitive components of binding were about 50% less in aged mice. Dose-response curves indicated that the effectiveness with which chloride enhanced binding was similar with age, even though the absolute increase in binding was less. The rate of dissociation of
3H-imipramine from cerebral cortical homogenates was similar with age and serotonin slowed the rate of dissociation equally at all ages. Possible mechanisms for the age-related increase in brain
3H-imipramine binding are discussed. Ion-sensitive binding is discussed in relationship to the current controversy surrounding desipramine-sensitive versus ion-sensitive binding. |
| doi_str_mv | 10.1016/0197-4580(86)90144-2 |
| format | Article |
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3H-imipramine was elevated in cerebral cortical, hypothalamic and hippocampal membranes from 24 month old male C57BL/6J mice. Cerebellar binding was constant with increasing age. There were no changes in the equilibrium dissociation constant (Kd) for
3H-imipramine in any brain region. The increase in the binding of
3H-imipramine induced by sodium and chloride ions
in vitro was diminished in cerebral cortical homogenates from aged mice; both the sodium-sensitive and chloride-sensitive components of binding were about 50% less in aged mice. Dose-response curves indicated that the effectiveness with which chloride enhanced binding was similar with age, even though the absolute increase in binding was less. The rate of dissociation of
3H-imipramine from cerebral cortical homogenates was similar with age and serotonin slowed the rate of dissociation equally at all ages. Possible mechanisms for the age-related increase in brain
3H-imipramine binding are discussed. Ion-sensitive binding is discussed in relationship to the current controversy surrounding desipramine-sensitive versus ion-sensitive binding.</description><identifier>ISSN: 0197-4580</identifier><identifier>EISSN: 1558-1497</identifier><identifier>DOI: 10.1016/0197-4580(86)90144-2</identifier><identifier>PMID: 3960267</identifier><identifier>CODEN: NEAGDO</identifier><language>eng</language><publisher>London: Elsevier Inc</publisher><subject>3H-imipramine ; Aging ; Animals ; Binding sites ; Biological and medical sciences ; Brain ; Brain - metabolism ; Chloride ; Chlorides - physiology ; Imipramine - metabolism ; Male ; Medical sciences ; Mice ; Mice, Inbred C57BL ; Neuropharmacology ; Pharmacology. Drug treatments ; Psychoanaleptics: cns stimulant, antidepressant agent, nootropic agent, mood stabilizer..., (alzheimer disease) ; Psychology. Psychoanalysis. Psychiatry ; Psychopharmacology ; Serotonin ; Serotonin - physiology ; Sodium ; Sodium - physiology ; Tissue Distribution ; Tritium</subject><ispartof>Neurobiology of aging, 1986-03, Vol.7 (2), p.83-87</ispartof><rights>1986</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2337-32c3eafb4e70398ba160502b314b4b63922ad670a3d6c0d0a40423358afb79c83</citedby><cites>FETCH-LOGICAL-c2337-32c3eafb4e70398ba160502b314b4b63922ad670a3d6c0d0a40423358afb79c83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0197458086901442$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8736871$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3960267$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Severson, James A.</creatorcontrib><title>3H-imipramine binding in aged mouse brain: regulation by ions and serotonin</title><title>Neurobiology of aging</title><addtitle>Neurobiol Aging</addtitle><description>The density of binding sites (Bmax) for
3H-imipramine was elevated in cerebral cortical, hypothalamic and hippocampal membranes from 24 month old male C57BL/6J mice. Cerebellar binding was constant with increasing age. There were no changes in the equilibrium dissociation constant (Kd) for
3H-imipramine in any brain region. The increase in the binding of
3H-imipramine induced by sodium and chloride ions
in vitro was diminished in cerebral cortical homogenates from aged mice; both the sodium-sensitive and chloride-sensitive components of binding were about 50% less in aged mice. Dose-response curves indicated that the effectiveness with which chloride enhanced binding was similar with age, even though the absolute increase in binding was less. The rate of dissociation of
3H-imipramine from cerebral cortical homogenates was similar with age and serotonin slowed the rate of dissociation equally at all ages. Possible mechanisms for the age-related increase in brain
3H-imipramine binding are discussed. Ion-sensitive binding is discussed in relationship to the current controversy surrounding desipramine-sensitive versus ion-sensitive binding.</description><subject>3H-imipramine</subject><subject>Aging</subject><subject>Animals</subject><subject>Binding sites</subject><subject>Biological and medical sciences</subject><subject>Brain</subject><subject>Brain - metabolism</subject><subject>Chloride</subject><subject>Chlorides - physiology</subject><subject>Imipramine - metabolism</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Neuropharmacology</subject><subject>Pharmacology. Drug treatments</subject><subject>Psychoanaleptics: cns stimulant, antidepressant agent, nootropic agent, mood stabilizer..., (alzheimer disease)</subject><subject>Psychology. Psychoanalysis. Psychiatry</subject><subject>Psychopharmacology</subject><subject>Serotonin</subject><subject>Serotonin - physiology</subject><subject>Sodium</subject><subject>Sodium - physiology</subject><subject>Tissue Distribution</subject><subject>Tritium</subject><issn>0197-4580</issn><issn>1558-1497</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1rGzEQhkVocJ2Pf9CCDiWkh01GHytpeyiEkCYlhl6Ss9BKY6Oyq3Ulu5B_Xzk2Pvb0wszzDsNDyCcGNwyYugXW6Ua2Bq6N-toBk7LhJ2TO2tY0THb6A5kfkY_krJTfAKClVjMyE50CrvScPIunJo5xnd0YE9I-phDTisZE3QoDHadtqdPsYvpGM662g9vEKdH-jdYo1KVAC-ZpM6WYLsjp0g0FLw95Tl5_PLzcPzWLX48_7-8WjedC6EZwL9Ate4kaRGd6xxS0wHvBZC97JTrOXVAanAjKQwAnQdZia2pHd96Ic3K1v7vO058tlo0dY_E4DC5h_ddqpVXHW1VBuQd9nkrJuLTrHEeX3ywDu3Nod4LsTpA1yr47tLzWPh_ub_sRw7F0kFb3Xw57V7wbltklH8sRM1ooo1nFvu8xrC7-Rsy2-IjJY4gZ_caGKf7_j39qj4uq</recordid><startdate>198603</startdate><enddate>198603</enddate><creator>Severson, James A.</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198603</creationdate><title>3H-imipramine binding in aged mouse brain: regulation by ions and serotonin</title><author>Severson, James A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2337-32c3eafb4e70398ba160502b314b4b63922ad670a3d6c0d0a40423358afb79c83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>3H-imipramine</topic><topic>Aging</topic><topic>Animals</topic><topic>Binding sites</topic><topic>Biological and medical sciences</topic><topic>Brain</topic><topic>Brain - metabolism</topic><topic>Chloride</topic><topic>Chlorides - physiology</topic><topic>Imipramine - metabolism</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Neuropharmacology</topic><topic>Pharmacology. Drug treatments</topic><topic>Psychoanaleptics: cns stimulant, antidepressant agent, nootropic agent, mood stabilizer..., (alzheimer disease)</topic><topic>Psychology. Psychoanalysis. Psychiatry</topic><topic>Psychopharmacology</topic><topic>Serotonin</topic><topic>Serotonin - physiology</topic><topic>Sodium</topic><topic>Sodium - physiology</topic><topic>Tissue Distribution</topic><topic>Tritium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Severson, James A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Neurobiology of aging</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Severson, James A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>3H-imipramine binding in aged mouse brain: regulation by ions and serotonin</atitle><jtitle>Neurobiology of aging</jtitle><addtitle>Neurobiol Aging</addtitle><date>1986-03</date><risdate>1986</risdate><volume>7</volume><issue>2</issue><spage>83</spage><epage>87</epage><pages>83-87</pages><issn>0197-4580</issn><eissn>1558-1497</eissn><coden>NEAGDO</coden><abstract>The density of binding sites (Bmax) for
3H-imipramine was elevated in cerebral cortical, hypothalamic and hippocampal membranes from 24 month old male C57BL/6J mice. Cerebellar binding was constant with increasing age. There were no changes in the equilibrium dissociation constant (Kd) for
3H-imipramine in any brain region. The increase in the binding of
3H-imipramine induced by sodium and chloride ions
in vitro was diminished in cerebral cortical homogenates from aged mice; both the sodium-sensitive and chloride-sensitive components of binding were about 50% less in aged mice. Dose-response curves indicated that the effectiveness with which chloride enhanced binding was similar with age, even though the absolute increase in binding was less. The rate of dissociation of
3H-imipramine from cerebral cortical homogenates was similar with age and serotonin slowed the rate of dissociation equally at all ages. Possible mechanisms for the age-related increase in brain
3H-imipramine binding are discussed. Ion-sensitive binding is discussed in relationship to the current controversy surrounding desipramine-sensitive versus ion-sensitive binding.</abstract><cop>London</cop><pub>Elsevier Inc</pub><pmid>3960267</pmid><doi>10.1016/0197-4580(86)90144-2</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0197-4580 |
| ispartof | Neurobiology of aging, 1986-03, Vol.7 (2), p.83-87 |
| issn | 0197-4580 1558-1497 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76769256 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 3H-imipramine Aging Animals Binding sites Biological and medical sciences Brain Brain - metabolism Chloride Chlorides - physiology Imipramine - metabolism Male Medical sciences Mice Mice, Inbred C57BL Neuropharmacology Pharmacology. Drug treatments Psychoanaleptics: cns stimulant, antidepressant agent, nootropic agent, mood stabilizer..., (alzheimer disease) Psychology. Psychoanalysis. Psychiatry Psychopharmacology Serotonin Serotonin - physiology Sodium Sodium - physiology Tissue Distribution Tritium |
| title | 3H-imipramine binding in aged mouse brain: regulation by ions and serotonin |
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