Isotopes as host blood markers to measure blood intake by feeding ticks (Acari: Ixodidae)
We have modified an experimental technique in which two different isotopes (Cr51 and I125) were used simultaneously as blood markers for determining the amount of blood removed by feeding ticks. This method enables us to measure separately and directly the volumes of red blood cells and plasma in ti...
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| Veröffentlicht in: | Journal of medical entomology 1994-07, Vol.31 (4), p.511-515 |
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| container_title | Journal of medical entomology |
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| creator | Rechav, Y Strydom, W.J Clarke, F.C Burger, L.B Mackie, A.J Fielden, L.J |
| description | We have modified an experimental technique in which two different isotopes (Cr51 and I125) were used simultaneously as blood markers for determining the amount of blood removed by feeding ticks. This method enables us to measure separately and directly the volumes of red blood cells and plasma in ticks and to calculate the ratio between the two parameters (concentrating ability). The concentration of red blood cells in nymphs of Amyblyomma hebraeum Kock and Rhipicephalus appendiculatus Neumann and in females of these species plus in those of Hyalomma truncatum Kock were found to be twice the amount measured in the labeled blood of goats. Concentrations of plasma in the ticks were approximately two-thirds of that measured in samples taken directly from these same goats. Small nonsignificant differences between the ability of various tick species to concentrate red blood cells and dilute host plasma were found. The advantage of this method is compared with that of the colorimetric and gravimetric methods, and its potential use is discussed |
| doi_str_mv | 10.1093/jmedent/31.4.511 |
| format | Article |
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This method enables us to measure separately and directly the volumes of red blood cells and plasma in ticks and to calculate the ratio between the two parameters (concentrating ability). The concentration of red blood cells in nymphs of Amyblyomma hebraeum Kock and Rhipicephalus appendiculatus Neumann and in females of these species plus in those of Hyalomma truncatum Kock were found to be twice the amount measured in the labeled blood of goats. Concentrations of plasma in the ticks were approximately two-thirds of that measured in samples taken directly from these same goats. Small nonsignificant differences between the ability of various tick species to concentrate red blood cells and dilute host plasma were found. The advantage of this method is compared with that of the colorimetric and gravimetric methods, and its potential use is discussed</description><identifier>ISSN: 0022-2585</identifier><identifier>EISSN: 1938-2928</identifier><identifier>DOI: 10.1093/jmedent/31.4.511</identifier><identifier>PMID: 7932595</identifier><language>eng</language><publisher>England</publisher><subject>Acari ; AMBLYOMMA HEBRAEUM ; Animals ; Biomarkers ; CAPRIN ; CAPRINOS ; Chromium Radioisotopes ; COMPORTEMENT ALIMENTAIRE ; Female ; Goats ; HABITOS ALIMENTARIOS ; HYALOMMA ; INGESTION DE ALIMENTOS ; Iodine Radioisotopes ; ISOTOPE ; ISOTOPOS ; Ixodidae ; Male ; PRISE ALIMENTAIRE (HOMME) ; RHIPICEPHALUS APPENDICULATUS ; SANG ; SANGRE ; Tick Infestations - blood ; Ticks - physiology</subject><ispartof>Journal of medical entomology, 1994-07, Vol.31 (4), p.511-515</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c344t-eb83271c2caeefc14cf4c6c87bcbc248d8ec133dccf6a2c8e647d115f170433b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7932595$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rechav, Y</creatorcontrib><creatorcontrib>Strydom, W.J</creatorcontrib><creatorcontrib>Clarke, F.C</creatorcontrib><creatorcontrib>Burger, L.B</creatorcontrib><creatorcontrib>Mackie, A.J</creatorcontrib><creatorcontrib>Fielden, L.J</creatorcontrib><title>Isotopes as host blood markers to measure blood intake by feeding ticks (Acari: Ixodidae)</title><title>Journal of medical entomology</title><addtitle>J Med Entomol</addtitle><description>We have modified an experimental technique in which two different isotopes (Cr51 and I125) were used simultaneously as blood markers for determining the amount of blood removed by feeding ticks. This method enables us to measure separately and directly the volumes of red blood cells and plasma in ticks and to calculate the ratio between the two parameters (concentrating ability). The concentration of red blood cells in nymphs of Amyblyomma hebraeum Kock and Rhipicephalus appendiculatus Neumann and in females of these species plus in those of Hyalomma truncatum Kock were found to be twice the amount measured in the labeled blood of goats. Concentrations of plasma in the ticks were approximately two-thirds of that measured in samples taken directly from these same goats. Small nonsignificant differences between the ability of various tick species to concentrate red blood cells and dilute host plasma were found. 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This method enables us to measure separately and directly the volumes of red blood cells and plasma in ticks and to calculate the ratio between the two parameters (concentrating ability). The concentration of red blood cells in nymphs of Amyblyomma hebraeum Kock and Rhipicephalus appendiculatus Neumann and in females of these species plus in those of Hyalomma truncatum Kock were found to be twice the amount measured in the labeled blood of goats. Concentrations of plasma in the ticks were approximately two-thirds of that measured in samples taken directly from these same goats. Small nonsignificant differences between the ability of various tick species to concentrate red blood cells and dilute host plasma were found. The advantage of this method is compared with that of the colorimetric and gravimetric methods, and its potential use is discussed</abstract><cop>England</cop><pmid>7932595</pmid><doi>10.1093/jmedent/31.4.511</doi><tpages>5</tpages></addata></record> |
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| identifier | ISSN: 0022-2585 |
| ispartof | Journal of medical entomology, 1994-07, Vol.31 (4), p.511-515 |
| issn | 0022-2585 1938-2928 |
| language | eng |
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| source | Oxford University Press Journals Digital Archive legacy; MEDLINE |
| subjects | Acari AMBLYOMMA HEBRAEUM Animals Biomarkers CAPRIN CAPRINOS Chromium Radioisotopes COMPORTEMENT ALIMENTAIRE Female Goats HABITOS ALIMENTARIOS HYALOMMA INGESTION DE ALIMENTOS Iodine Radioisotopes ISOTOPE ISOTOPOS Ixodidae Male PRISE ALIMENTAIRE (HOMME) RHIPICEPHALUS APPENDICULATUS SANG SANGRE Tick Infestations - blood Ticks - physiology |
| title | Isotopes as host blood markers to measure blood intake by feeding ticks (Acari: Ixodidae) |
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