[40] Isoprenylation of γ subunits and G-protein effectors

Several approaches have been developed for the identification of isoprenyl groups on modified proteins. The first, and most rigorous, involves chemical cleavage of the modifying isoprenyl group with Raney nickel, followed by gas chromatography-coupled mass spectrometric (GC–MS) analysis of the produ...

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Veröffentlicht in:	Methods in Enzymology 1994, Vol.237, p.509-519
Hauptverfasser:	Fung, Bernard K.-K, Anant, Janmeet S, Lin, Wun-Chen, Ong, Olivia C, Yamane, Harvey K
Format:	Artikel
Sprache:	eng
Schlagworte:	Alkyl and Aryl Transferases Amino Acid Sequence Animals Cysteine - analogs & derivatives Cysteine - chemical synthesis Cysteine - metabolism Diterpenes - chemical synthesis Diterpenes - metabolism Electrophoresis, Polyacrylamide Gel - methods Farnesyltranstransferase GTP-Binding Proteins - isolation & purification GTP-Binding Proteins - metabolism Macromolecular Substances Mevalonic Acid - metabolism Molecular Sequence Data Molecular Weight Protein Prenylation Radioisotope Dilution Technique Rats Retina - metabolism Rod Cell Outer Segment - metabolism Transferases - metabolism Tritium
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