Creatine kinase isoenzymes in spermatozoa
Two isoforms of creatine kinase (CK, E.C. 2.7.3.2), the brain type BB-CK and the mitochondrial-bound MiMi-CK, as well as adenylate kinase (myokinase, E.C. 2.7.4.3) were identified in washed spermatozoa from chicken and man by cellulose polyacetate electrophoresis and immunoblots. BB-CK was localized...
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| Veröffentlicht in: | Journal of muscle research and cell motility 1986-02, Vol.7 (1), p.25-34 |
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| container_title | Journal of muscle research and cell motility |
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| creator | WALLIMANN, I MOSER, H ZURBRIGGEN, B WEGMANN, G EPPENBERGER, H. M |
| description | Two isoforms of creatine kinase (CK, E.C. 2.7.3.2), the brain type BB-CK and the mitochondrial-bound MiMi-CK, as well as adenylate kinase (myokinase, E.C. 2.7.4.3) were identified in washed spermatozoa from chicken and man by cellulose polyacetate electrophoresis and immunoblots. BB-CK was localized by indirect immunofluorescence staining within the sperm tail but not in the head portion. MiMi-CK is confined to the midpiece region rich in mitochondria and has been localized directly by immunogold staining within the mitochondria. In contrast to chicken, seminal plasma from man was also found to contain considerable amounts of BB-CK. Total creatine content of spermatozoa (8-15 mM) and seminal plasma (3.8 +/- 0.4 mM) as well as preliminary experiments with metabolic blockers indicate a dependence of sperm motility on CK and phosphoryl creatine (CP). The presence of two CK isoforms located in different 'compartments' of spermatozoa suggests a CP-shuttle in sperm similar to that described for cross-striated muscle. |
| doi_str_mv | 10.1007/BF01756199 |
| format | Article |
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The presence of two CK isoforms located in different 'compartments' of spermatozoa suggests a CP-shuttle in sperm similar to that described for cross-striated muscle.</description><identifier>ISSN: 0142-4319</identifier><identifier>EISSN: 1573-2657</identifier><identifier>DOI: 10.1007/BF01756199</identifier><identifier>PMID: 3514665</identifier><identifier>CODEN: JMRMD3</identifier><language>eng</language><publisher>London: Kluwer Academic Publishers</publisher><subject>Animals ; Biological and medical sciences ; Chickens ; Creatine Kinase - isolation & purification ; Creatine Kinase - metabolism ; Fluorescent Antibody Technique ; Fundamental and applied biological sciences. Psychology ; Humans ; Isoenzymes ; Male ; Mammalian male genital system ; Microscopy, Electron ; Mitochondria - enzymology ; Mitochondria - ultrastructure ; Molecular Weight ; Morphology. 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M</creatorcontrib><title>Creatine kinase isoenzymes in spermatozoa</title><title>Journal of muscle research and cell motility</title><addtitle>J Muscle Res Cell Motil</addtitle><description>Two isoforms of creatine kinase (CK, E.C. 2.7.3.2), the brain type BB-CK and the mitochondrial-bound MiMi-CK, as well as adenylate kinase (myokinase, E.C. 2.7.4.3) were identified in washed spermatozoa from chicken and man by cellulose polyacetate electrophoresis and immunoblots. BB-CK was localized by indirect immunofluorescence staining within the sperm tail but not in the head portion. MiMi-CK is confined to the midpiece region rich in mitochondria and has been localized directly by immunogold staining within the mitochondria. In contrast to chicken, seminal plasma from man was also found to contain considerable amounts of BB-CK. Total creatine content of spermatozoa (8-15 mM) and seminal plasma (3.8 +/- 0.4 mM) as well as preliminary experiments with metabolic blockers indicate a dependence of sperm motility on CK and phosphoryl creatine (CP). The presence of two CK isoforms located in different 'compartments' of spermatozoa suggests a CP-shuttle in sperm similar to that described for cross-striated muscle.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Chickens</subject><subject>Creatine Kinase - isolation & purification</subject><subject>Creatine Kinase - metabolism</subject><subject>Fluorescent Antibody Technique</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Isoenzymes</subject><subject>Male</subject><subject>Mammalian male genital system</subject><subject>Microscopy, Electron</subject><subject>Mitochondria - enzymology</subject><subject>Mitochondria - ultrastructure</subject><subject>Molecular Weight</subject><subject>Morphology. Physiology</subject><subject>Semen - enzymology</subject><subject>Species Specificity</subject><subject>Spermatozoa - cytology</subject><subject>Spermatozoa - enzymology</subject><subject>Spermatozoa - ultrastructure</subject><subject>Vertebrates: reproduction</subject><issn>0142-4319</issn><issn>1573-2657</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkEtLAzEUhYMotVY37oVZiKAwmpub51KLVaHgRtdDmslAdB41mS7aX-9IhwoHzuJ8nMVHyCXQe6BUPTwtKCghwZgjMgWhMGdSqGMypcBZzhHMKTlL6YtSKgxjEzJBAVxKMSW38-htH1qffYfWJp-F1Pl2t218ykKbpbWPje27XWfPyUll6-Qvxp6Rz8Xzx_w1X76_vM0fl7lDgD6XTCPKshSOl3pFvTZAuZYUOZM4RHBQpXNMSDTCKVUxr1AxY1aGV5YbnJGb_e86dj8bn_qiCcn5urat7zapUFJJjloO4N0edLFLKfqqWMfQ2LgtgBZ_Xop_LwN8Nb5uVo0vD-goYtivx90mZ-sq2taFdMA0Ba0R8BfHh2bD</recordid><startdate>19860201</startdate><enddate>19860201</enddate><creator>WALLIMANN, I</creator><creator>MOSER, H</creator><creator>ZURBRIGGEN, B</creator><creator>WEGMANN, G</creator><creator>EPPENBERGER, H. 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Psychology</topic><topic>Humans</topic><topic>Isoenzymes</topic><topic>Male</topic><topic>Mammalian male genital system</topic><topic>Microscopy, Electron</topic><topic>Mitochondria - enzymology</topic><topic>Mitochondria - ultrastructure</topic><topic>Molecular Weight</topic><topic>Morphology. Physiology</topic><topic>Semen - enzymology</topic><topic>Species Specificity</topic><topic>Spermatozoa - cytology</topic><topic>Spermatozoa - enzymology</topic><topic>Spermatozoa - ultrastructure</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>WALLIMANN, I</creatorcontrib><creatorcontrib>MOSER, H</creatorcontrib><creatorcontrib>ZURBRIGGEN, B</creatorcontrib><creatorcontrib>WEGMANN, G</creatorcontrib><creatorcontrib>EPPENBERGER, H. 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M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Creatine kinase isoenzymes in spermatozoa</atitle><jtitle>Journal of muscle research and cell motility</jtitle><addtitle>J Muscle Res Cell Motil</addtitle><date>1986-02-01</date><risdate>1986</risdate><volume>7</volume><issue>1</issue><spage>25</spage><epage>34</epage><pages>25-34</pages><issn>0142-4319</issn><eissn>1573-2657</eissn><coden>JMRMD3</coden><abstract>Two isoforms of creatine kinase (CK, E.C. 2.7.3.2), the brain type BB-CK and the mitochondrial-bound MiMi-CK, as well as adenylate kinase (myokinase, E.C. 2.7.4.3) were identified in washed spermatozoa from chicken and man by cellulose polyacetate electrophoresis and immunoblots. BB-CK was localized by indirect immunofluorescence staining within the sperm tail but not in the head portion. MiMi-CK is confined to the midpiece region rich in mitochondria and has been localized directly by immunogold staining within the mitochondria. In contrast to chicken, seminal plasma from man was also found to contain considerable amounts of BB-CK. Total creatine content of spermatozoa (8-15 mM) and seminal plasma (3.8 +/- 0.4 mM) as well as preliminary experiments with metabolic blockers indicate a dependence of sperm motility on CK and phosphoryl creatine (CP). The presence of two CK isoforms located in different 'compartments' of spermatozoa suggests a CP-shuttle in sperm similar to that described for cross-striated muscle.</abstract><cop>London</cop><cop>Boston</cop><cop>Dordrecht</cop><pub>Kluwer Academic Publishers</pub><pmid>3514665</pmid><doi>10.1007/BF01756199</doi><tpages>10</tpages></addata></record> |
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| language | eng |
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| subjects | Animals Biological and medical sciences Chickens Creatine Kinase - isolation & purification Creatine Kinase - metabolism Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Humans Isoenzymes Male Mammalian male genital system Microscopy, Electron Mitochondria - enzymology Mitochondria - ultrastructure Molecular Weight Morphology. Physiology Semen - enzymology Species Specificity Spermatozoa - cytology Spermatozoa - enzymology Spermatozoa - ultrastructure Vertebrates: reproduction |
| title | Creatine kinase isoenzymes in spermatozoa |
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