A murine monoclonal antibody (RV3-27) raised against isolated human placental endogenous retroviral particles and reactive with syncytiotrophoblast
Particles with the characteristic shape of enveloped retroviral particles and maximal specific reverse transcriptase (RTase) activity at buoyant density of 1.15–1.17 g/ml have been isolated from human first-trimester chorionic villous tissue. Murine monoclonal antibodies (mAbs) to these isolated par...
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| Veröffentlicht in: | Journal of reproductive immunology 1994-03, Vol.26 (2), p.75-95 |
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| container_title | Journal of reproductive immunology |
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| creator | Mwenda, J.M. Maher, P.M. Melling, G.C. Lyden, T.W. Johnson, P.M. |
| description | Particles with the characteristic shape of enveloped retroviral particles and maximal specific reverse transcriptase (RTase) activity at buoyant density of 1.15–1.17 g/ml have been isolated from human first-trimester chorionic villous tissue. Murine monoclonal antibodies (mAbs) to these isolated particles were generated. One IgM mAb (RV3-27) showed granular staining of cytoplasmic structures within syncytiotrophoblast by immunohistochemistry. Immunoelectron microscopic studies have demonstrated focal localisation to small submembranous regions of syncytiotrophoblast, as well as reaction with detergent-disrupted isolated placental retroviral-like particles. The RV3-27 mAb did not stain other human tissues in this focal manner, although increased generalised cytoplasmic staining was not uncommon; also, this mAb did not react strongly with the surface or cytoplasm of a variety of human cell lines (including choriocarcinoma cells). Immunoblotting and HPLC analyses have indicated the reactive placental antigen to be a 17–25 kDa protein. It is suggested that the RV3-27 mAb may be reactive with a syncytiotrophoblast antigen encoded by an endogenous retroviral sequence. |
| doi_str_mv | 10.1016/0165-0378(94)90033-7 |
| format | Article |
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Murine monoclonal antibodies (mAbs) to these isolated particles were generated. One IgM mAb (RV3-27) showed granular staining of cytoplasmic structures within syncytiotrophoblast by immunohistochemistry. Immunoelectron microscopic studies have demonstrated focal localisation to small submembranous regions of syncytiotrophoblast, as well as reaction with detergent-disrupted isolated placental retroviral-like particles. The RV3-27 mAb did not stain other human tissues in this focal manner, although increased generalised cytoplasmic staining was not uncommon; also, this mAb did not react strongly with the surface or cytoplasm of a variety of human cell lines (including choriocarcinoma cells). Immunoblotting and HPLC analyses have indicated the reactive placental antigen to be a 17–25 kDa protein. It is suggested that the RV3-27 mAb may be reactive with a syncytiotrophoblast antigen encoded by an endogenous retroviral sequence.</description><identifier>ISSN: 0165-0378</identifier><identifier>EISSN: 1872-7603</identifier><identifier>DOI: 10.1016/0165-0378(94)90033-7</identifier><identifier>PMID: 7523669</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Animals ; Antibodies, Monoclonal - immunology ; Antibodies, Viral - immunology ; Choriocarcinoma - pathology ; Choriocarcinoma - virology ; Choriocarcinoma cells ; Chorionic Villi - virology ; Endogenous retrovirus ; Extraembryonic Membranes - immunology ; Female ; Humans ; Immunoglobulin M - immunology ; Immunologic Techniques ; Mice ; Mice, Inbred BALB C ; Microscopy, Electron ; Monoclonal antibody (RV3-27) ; Neoplasms - pathology ; Organ Specificity ; Placenta ; Retroviridae - enzymology ; Retroviridae - immunology ; Retroviridae - isolation & purification ; Retroviridae - ultrastructure ; RNA-Directed DNA Polymerase - analysis ; Syncytiotrophoblast ; Trophoblasts - immunology ; Trophoblasts - virology ; Tumor Cells, Cultured ; Uterine Neoplasms - pathology ; Uterine Neoplasms - virology ; Viral Proteins - analysis</subject><ispartof>Journal of reproductive immunology, 1994-03, Vol.26 (2), p.75-95</ispartof><rights>1994</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-80f992437184c6df388cb980f4b263954971fbbfdbdc2e90f15c73c2b5a32ca03</citedby><cites>FETCH-LOGICAL-c388t-80f992437184c6df388cb980f4b263954971fbbfdbdc2e90f15c73c2b5a32ca03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0165-0378(94)90033-7$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7523669$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mwenda, J.M.</creatorcontrib><creatorcontrib>Maher, P.M.</creatorcontrib><creatorcontrib>Melling, G.C.</creatorcontrib><creatorcontrib>Lyden, T.W.</creatorcontrib><creatorcontrib>Johnson, P.M.</creatorcontrib><title>A murine monoclonal antibody (RV3-27) raised against isolated human placental endogenous retroviral particles and reactive with syncytiotrophoblast</title><title>Journal of reproductive immunology</title><addtitle>J Reprod Immunol</addtitle><description>Particles with the characteristic shape of enveloped retroviral particles and maximal specific reverse transcriptase (RTase) activity at buoyant density of 1.15–1.17 g/ml have been isolated from human first-trimester chorionic villous tissue. Murine monoclonal antibodies (mAbs) to these isolated particles were generated. One IgM mAb (RV3-27) showed granular staining of cytoplasmic structures within syncytiotrophoblast by immunohistochemistry. Immunoelectron microscopic studies have demonstrated focal localisation to small submembranous regions of syncytiotrophoblast, as well as reaction with detergent-disrupted isolated placental retroviral-like particles. The RV3-27 mAb did not stain other human tissues in this focal manner, although increased generalised cytoplasmic staining was not uncommon; also, this mAb did not react strongly with the surface or cytoplasm of a variety of human cell lines (including choriocarcinoma cells). Immunoblotting and HPLC analyses have indicated the reactive placental antigen to be a 17–25 kDa protein. It is suggested that the RV3-27 mAb may be reactive with a syncytiotrophoblast antigen encoded by an endogenous retroviral sequence.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antibodies, Viral - immunology</subject><subject>Choriocarcinoma - pathology</subject><subject>Choriocarcinoma - virology</subject><subject>Choriocarcinoma cells</subject><subject>Chorionic Villi - virology</subject><subject>Endogenous retrovirus</subject><subject>Extraembryonic Membranes - immunology</subject><subject>Female</subject><subject>Humans</subject><subject>Immunoglobulin M - immunology</subject><subject>Immunologic Techniques</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microscopy, Electron</subject><subject>Monoclonal antibody (RV3-27)</subject><subject>Neoplasms - pathology</subject><subject>Organ Specificity</subject><subject>Placenta</subject><subject>Retroviridae - enzymology</subject><subject>Retroviridae - immunology</subject><subject>Retroviridae - isolation & purification</subject><subject>Retroviridae - ultrastructure</subject><subject>RNA-Directed DNA Polymerase - analysis</subject><subject>Syncytiotrophoblast</subject><subject>Trophoblasts - immunology</subject><subject>Trophoblasts - virology</subject><subject>Tumor Cells, Cultured</subject><subject>Uterine Neoplasms - pathology</subject><subject>Uterine Neoplasms - virology</subject><subject>Viral Proteins - analysis</subject><issn>0165-0378</issn><issn>1872-7603</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUdtqFDEYDqLUbfUNFHIl7cVoDjOTyY1QilWhIIh6G3L4pxuZScYks2Wfwxc26y69VMJP4DuF_B9Cryh5Swnt39XpGsLFcCnbK0kI5414gjZ0EKwRPeFP0eZR8hyd5_yTECqIpGfoTHSM973coN_XeF6TD4DnGKKdYtAT1qF4E90eX379wRsmrnDSPoPD-l77kAv2OU66VGC7zjrgZdIWQqlOCC7eQ4hrxglKijufKrroVLydINdkVwlti98BfvBli_M-2H3xsYqXbTSTzuUFejbqKcPL032Bvt9--Hbzqbn78vHzzfVdY_kwlGYgo5Ss5YIOre3dWEFrZEVbw3ouu1YKOhozOuMsA0lG2lnBLTOd5sxqwi_Qm2PukuKvFXJRs88WpkkHqD9QohcdZ_X8T0j7XgyMDlXYHoU2xZwTjGpJftZpryhRh9LUoRF1aETJVv0tTYlqe33KX80M7tF0aqny74881G3sPCSVrYdgwfkEtigX_b8f-AM_Yakv</recordid><startdate>19940301</startdate><enddate>19940301</enddate><creator>Mwenda, J.M.</creator><creator>Maher, P.M.</creator><creator>Melling, G.C.</creator><creator>Lyden, T.W.</creator><creator>Johnson, P.M.</creator><general>Elsevier Ireland Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19940301</creationdate><title>A murine monoclonal antibody (RV3-27) raised against isolated human placental endogenous retroviral particles and reactive with syncytiotrophoblast</title><author>Mwenda, J.M. ; Maher, P.M. ; Melling, G.C. ; Lyden, T.W. ; Johnson, P.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-80f992437184c6df388cb980f4b263954971fbbfdbdc2e90f15c73c2b5a32ca03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antibodies, Viral - immunology</topic><topic>Choriocarcinoma - pathology</topic><topic>Choriocarcinoma - virology</topic><topic>Choriocarcinoma cells</topic><topic>Chorionic Villi - virology</topic><topic>Endogenous retrovirus</topic><topic>Extraembryonic Membranes - immunology</topic><topic>Female</topic><topic>Humans</topic><topic>Immunoglobulin M - immunology</topic><topic>Immunologic Techniques</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microscopy, Electron</topic><topic>Monoclonal antibody (RV3-27)</topic><topic>Neoplasms - pathology</topic><topic>Organ Specificity</topic><topic>Placenta</topic><topic>Retroviridae - enzymology</topic><topic>Retroviridae - immunology</topic><topic>Retroviridae - isolation & purification</topic><topic>Retroviridae - ultrastructure</topic><topic>RNA-Directed DNA Polymerase - analysis</topic><topic>Syncytiotrophoblast</topic><topic>Trophoblasts - immunology</topic><topic>Trophoblasts - virology</topic><topic>Tumor Cells, Cultured</topic><topic>Uterine Neoplasms - pathology</topic><topic>Uterine Neoplasms - virology</topic><topic>Viral Proteins - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mwenda, J.M.</creatorcontrib><creatorcontrib>Maher, P.M.</creatorcontrib><creatorcontrib>Melling, G.C.</creatorcontrib><creatorcontrib>Lyden, T.W.</creatorcontrib><creatorcontrib>Johnson, P.M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of reproductive immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mwenda, J.M.</au><au>Maher, P.M.</au><au>Melling, G.C.</au><au>Lyden, T.W.</au><au>Johnson, P.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A murine monoclonal antibody (RV3-27) raised against isolated human placental endogenous retroviral particles and reactive with syncytiotrophoblast</atitle><jtitle>Journal of reproductive immunology</jtitle><addtitle>J Reprod Immunol</addtitle><date>1994-03-01</date><risdate>1994</risdate><volume>26</volume><issue>2</issue><spage>75</spage><epage>95</epage><pages>75-95</pages><issn>0165-0378</issn><eissn>1872-7603</eissn><abstract>Particles with the characteristic shape of enveloped retroviral particles and maximal specific reverse transcriptase (RTase) activity at buoyant density of 1.15–1.17 g/ml have been isolated from human first-trimester chorionic villous tissue. Murine monoclonal antibodies (mAbs) to these isolated particles were generated. One IgM mAb (RV3-27) showed granular staining of cytoplasmic structures within syncytiotrophoblast by immunohistochemistry. Immunoelectron microscopic studies have demonstrated focal localisation to small submembranous regions of syncytiotrophoblast, as well as reaction with detergent-disrupted isolated placental retroviral-like particles. The RV3-27 mAb did not stain other human tissues in this focal manner, although increased generalised cytoplasmic staining was not uncommon; also, this mAb did not react strongly with the surface or cytoplasm of a variety of human cell lines (including choriocarcinoma cells). Immunoblotting and HPLC analyses have indicated the reactive placental antigen to be a 17–25 kDa protein. It is suggested that the RV3-27 mAb may be reactive with a syncytiotrophoblast antigen encoded by an endogenous retroviral sequence.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>7523669</pmid><doi>10.1016/0165-0378(94)90033-7</doi><tpages>21</tpages></addata></record> |
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| ispartof | Journal of reproductive immunology, 1994-03, Vol.26 (2), p.75-95 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Antibodies, Monoclonal - immunology Antibodies, Viral - immunology Choriocarcinoma - pathology Choriocarcinoma - virology Choriocarcinoma cells Chorionic Villi - virology Endogenous retrovirus Extraembryonic Membranes - immunology Female Humans Immunoglobulin M - immunology Immunologic Techniques Mice Mice, Inbred BALB C Microscopy, Electron Monoclonal antibody (RV3-27) Neoplasms - pathology Organ Specificity Placenta Retroviridae - enzymology Retroviridae - immunology Retroviridae - isolation & purification Retroviridae - ultrastructure RNA-Directed DNA Polymerase - analysis Syncytiotrophoblast Trophoblasts - immunology Trophoblasts - virology Tumor Cells, Cultured Uterine Neoplasms - pathology Uterine Neoplasms - virology Viral Proteins - analysis |
| title | A murine monoclonal antibody (RV3-27) raised against isolated human placental endogenous retroviral particles and reactive with syncytiotrophoblast |
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