Determination of free choline in plasma and erythrocyte samples and choline derived from membrane phosphatidylcholine by a chemiluminescence method
A sensitive chemiluminescence method for assay of choline which has been developed for analysis of erythrocyte and plasma levels of choline is reported here. This method includes a charcoal purification step which yields consistent results with plasma and erythrocyte extracts. Further, choline deriv...
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| Veröffentlicht in: | Analytical biochemistry 1986, Vol.152 (1), p.178-182 |
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| container_title | Analytical biochemistry |
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| creator | Das, Indrajit de Belleroche, Jacqueline Moore, Christopher J. Clifford Rose, Frank |
| description | A sensitive chemiluminescence method for assay of choline which has been developed for analysis of erythrocyte and plasma levels of choline is reported here. This method includes a charcoal purification step which yields consistent results with plasma and erythrocyte extracts. Further, choline derived from membrane phosphatidylcholine may also be measured by an extension of this method following digestion with phospholipase D. This method has been used to study abnormal levels of erythrocyte choline that occur in cluster headache patients compared to control subjects and migraine patients. In addition, the time course of changes in plasma and erythrocyte choline following a fatty meal have been monitored. Plasma choline levels rise to a maximum between 1 and 3 h after the meal and this is followed by a rise in erythrocyte choline levels which are maximal 3 h after the meal. |
| doi_str_mv | 10.1016/0003-2697(86)90138-7 |
| format | Article |
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This method includes a charcoal purification step which yields consistent results with plasma and erythrocyte extracts. Further, choline derived from membrane phosphatidylcholine may also be measured by an extension of this method following digestion with phospholipase D. This method has been used to study abnormal levels of erythrocyte choline that occur in cluster headache patients compared to control subjects and migraine patients. In addition, the time course of changes in plasma and erythrocyte choline following a fatty meal have been monitored. 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This method includes a charcoal purification step which yields consistent results with plasma and erythrocyte extracts. Further, choline derived from membrane phosphatidylcholine may also be measured by an extension of this method following digestion with phospholipase D. This method has been used to study abnormal levels of erythrocyte choline that occur in cluster headache patients compared to control subjects and migraine patients. In addition, the time course of changes in plasma and erythrocyte choline following a fatty meal have been monitored. Plasma choline levels rise to a maximum between 1 and 3 h after the meal and this is followed by a rise in erythrocyte choline levels which are maximal 3 h after the meal.</description><subject>Applied sciences</subject><subject>assays</subject><subject>chemiluminescence</subject><subject>Cholesterol - blood</subject><subject>choline</subject><subject>Choline - blood</subject><subject>Dietary Fats - pharmacology</subject><subject>erythrocytes</subject><subject>Erythrocytes - analysis</subject><subject>Exact sciences and technology</subject><subject>Humans</subject><subject>Luminescent Measurements</subject><subject>man</subject><subject>Membrane Lipids - metabolism</subject><subject>Other techniques and industries</subject><subject>phosphatidylcholine</subject><subject>Phosphatidylcholines - metabolism</subject><subject>Phosphorus - blood</subject><subject>plasma</subject><subject>Plasma - analysis</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUuLFDEUhYMoY8_oP1DIQkQXpXlVHhtBxvEBA250HdLJLTpSqZRJ9UD9Dv-w6emeXuoqcO93Tg73IPSCkneUUPmeEMI7Jo16o-VbQyjXnXqENpQY2RFOzGO0OSNP0WWtvwihVPTyAl1w0wvCzQb9-QQLlBQnt8Q84TzgoQBgv8tjnADHCc-jq8lhNwUMZV12Jft1AVxdmkeo9_MHOkCJdxCaRU44QdoW16bzLtd51_zDOj6Q2xW7JoMUx337HKqHyUPTLLscnqEngxsrPD-9V-jn55sf11-72-9fvl1_vO28oGrpBq21A-21lMQYykCRwTE3MKmZor0QwHjopePGi55xGdigpVKMgvew5Y5foddH37nk33uoi02xBRnHljrvq1VS9VRo8V-QCq4bRxsojqAvudYCg51LTK6slhJ7KM0eGrGHRqyW9r40q5rs5cl_v00QzqJTS23_6rR31btxaGf1sZ4xTZjWhjfswxGDdrS7CMVWHw93DbGAX2zI8d85_gKnr7Wf</recordid><startdate>1986</startdate><enddate>1986</enddate><creator>Das, Indrajit</creator><creator>de Belleroche, Jacqueline</creator><creator>Moore, Christopher J.</creator><creator>Clifford Rose, Frank</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>1986</creationdate><title>Determination of free choline in plasma and erythrocyte samples and choline derived from membrane phosphatidylcholine by a chemiluminescence method</title><author>Das, Indrajit ; de Belleroche, Jacqueline ; Moore, Christopher J. ; Clifford Rose, Frank</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-f888ae8c86609912e70fa2af268271544e23d56a39c45236d2f867721ecceb3a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Applied sciences</topic><topic>assays</topic><topic>chemiluminescence</topic><topic>Cholesterol - blood</topic><topic>choline</topic><topic>Choline - blood</topic><topic>Dietary Fats - pharmacology</topic><topic>erythrocytes</topic><topic>Erythrocytes - analysis</topic><topic>Exact sciences and technology</topic><topic>Humans</topic><topic>Luminescent Measurements</topic><topic>man</topic><topic>Membrane Lipids - metabolism</topic><topic>Other techniques and industries</topic><topic>phosphatidylcholine</topic><topic>Phosphatidylcholines - metabolism</topic><topic>Phosphorus - blood</topic><topic>plasma</topic><topic>Plasma - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Das, Indrajit</creatorcontrib><creatorcontrib>de Belleroche, Jacqueline</creatorcontrib><creatorcontrib>Moore, Christopher J.</creatorcontrib><creatorcontrib>Clifford Rose, Frank</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Das, Indrajit</au><au>de Belleroche, Jacqueline</au><au>Moore, Christopher J.</au><au>Clifford Rose, Frank</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of free choline in plasma and erythrocyte samples and choline derived from membrane phosphatidylcholine by a chemiluminescence method</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1986</date><risdate>1986</risdate><volume>152</volume><issue>1</issue><spage>178</spage><epage>182</epage><pages>178-182</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><coden>ANBCA2</coden><abstract>A sensitive chemiluminescence method for assay of choline which has been developed for analysis of erythrocyte and plasma levels of choline is reported here. This method includes a charcoal purification step which yields consistent results with plasma and erythrocyte extracts. Further, choline derived from membrane phosphatidylcholine may also be measured by an extension of this method following digestion with phospholipase D. This method has been used to study abnormal levels of erythrocyte choline that occur in cluster headache patients compared to control subjects and migraine patients. In addition, the time course of changes in plasma and erythrocyte choline following a fatty meal have been monitored. Plasma choline levels rise to a maximum between 1 and 3 h after the meal and this is followed by a rise in erythrocyte choline levels which are maximal 3 h after the meal.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>3954039</pmid><doi>10.1016/0003-2697(86)90138-7</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2697 |
| ispartof | Analytical biochemistry, 1986, Vol.152 (1), p.178-182 |
| issn | 0003-2697 1096-0309 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76751484 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Applied sciences assays chemiluminescence Cholesterol - blood choline Choline - blood Dietary Fats - pharmacology erythrocytes Erythrocytes - analysis Exact sciences and technology Humans Luminescent Measurements man Membrane Lipids - metabolism Other techniques and industries phosphatidylcholine Phosphatidylcholines - metabolism Phosphorus - blood plasma Plasma - analysis |
| title | Determination of free choline in plasma and erythrocyte samples and choline derived from membrane phosphatidylcholine by a chemiluminescence method |
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