The global transcriptional regulators, SSN6 and TUP1, play distinct roles in the establishment of a repressive chromatin structure
Repression of a-cell specific gene expression in yeast alpha cells requires MAT alpha 2 and MCM1, as well as two global repressors, SSN6 and TUP1. Previous studies demonstrated that nucleosomes positioned adjacent to the alpha 2/MCM1 operator in alpha cells directly contribute to repression. To inve...
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| Veröffentlicht in: | Genes & development 1994-06, Vol.8 (12), p.1400-1410 |
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| creator | Cooper, J P Roth, S Y Simpson, R T |
| description | Repression of a-cell specific gene expression in yeast alpha cells requires MAT alpha 2 and MCM1, as well as two global repressors, SSN6 and TUP1. Previous studies demonstrated that nucleosomes positioned adjacent to the alpha 2/MCM1 operator in alpha cells directly contribute to repression. To investigate the possibility that SSN6 and TUP1 provide a link between MAT alpha 2/MCM1 and neighboring histones, nucleosome locations were examined in ssn6 and tup1 alpha cells. In both cases, nucleosome positions downstream of the operator were disrupted, and the severity of the disruption correlated with the degree of derepression. Nevertheless, the observed changes in chromatin structure were not dependent on transcription. Our data strongly indicate that SSN6 and TUP1 directly organize repressive regions of chromatin. |
| doi_str_mv | 10.1101/gad.8.12.1400 |
| format | Article |
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Previous studies demonstrated that nucleosomes positioned adjacent to the alpha 2/MCM1 operator in alpha cells directly contribute to repression. To investigate the possibility that SSN6 and TUP1 provide a link between MAT alpha 2/MCM1 and neighboring histones, nucleosome locations were examined in ssn6 and tup1 alpha cells. In both cases, nucleosome positions downstream of the operator were disrupted, and the severity of the disruption correlated with the degree of derepression. Nevertheless, the observed changes in chromatin structure were not dependent on transcription. 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Our data strongly indicate that SSN6 and TUP1 directly organize repressive regions of chromatin.</description><subject>ATP-Binding Cassette Transporters - genetics</subject><subject>DNA, Circular - genetics</subject><subject>DNA-Binding Proteins - genetics</subject><subject>Fungal Proteins - genetics</subject><subject>Gene Expression Regulation, Fungal</subject><subject>Glycoproteins</subject><subject>Mating Factor</subject><subject>Minichromosome Maintenance 1 Protein</subject><subject>Models, Genetic</subject><subject>Nuclear Proteins</subject><subject>Nucleic Acid Conformation</subject><subject>Nucleosomes - metabolism</subject><subject>Operator Regions, Genetic - genetics</subject><subject>Peptides - genetics</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Recombinant Fusion Proteins - biosynthesis</subject><subject>Repressor Proteins - metabolism</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae Proteins</subject><subject>Sequence Deletion</subject><subject>Transcription Factors - genetics</subject><subject>Transcription, Genetic</subject><issn>0890-9369</issn><issn>1549-5477</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkTFrHDEQRkWIcS62y5QGVam8Z0mnlVZlMI4dMEnA51popdk7Ge1qI2kNbvPLI3NHWlfDMG8eM3wIfaFkTSmh1zvj1t2asjXlhHxAK9py1bRcyo9oRTpFGrUR6hP6nPMzIUQQIU7RqVRMSE5W6O92D3gXYm8CLslM2SY_Fx-n2ifYLcGUmPIVfnz8KbCZHN4-_aZXeA7mFTufi59swSkGyNhPuFQZ5GL64PN-hKngOGBTRXOCnP0LYLtPcTR1DeeSFluWBOfoZDAhw8WxnqGn77fbm_vm4dfdj5tvD43ljJf6hhmAQetk3xIp25ZQ0g9CiU501nVD7xiHliliWV-nfGCskxxU75wD1242Z-jrwTun-GepZ-rRZwshmAnikrUUsjpV9y5IhaKUcVHB5gDaFHNOMOg5-dGkV02JfgtH13B0pynTb-FU_vIoXvoR3H_6mMbmH1mtjEE</recordid><startdate>19940615</startdate><enddate>19940615</enddate><creator>Cooper, J P</creator><creator>Roth, S Y</creator><creator>Simpson, R T</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>19940615</creationdate><title>The global transcriptional regulators, SSN6 and TUP1, play distinct roles in the establishment of a repressive chromatin structure</title><author>Cooper, J P ; 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| issn | 0890-9369 1549-5477 |
| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | ATP-Binding Cassette Transporters - genetics DNA, Circular - genetics DNA-Binding Proteins - genetics Fungal Proteins - genetics Gene Expression Regulation, Fungal Glycoproteins Mating Factor Minichromosome Maintenance 1 Protein Models, Genetic Nuclear Proteins Nucleic Acid Conformation Nucleosomes - metabolism Operator Regions, Genetic - genetics Peptides - genetics Promoter Regions, Genetic - genetics Recombinant Fusion Proteins - biosynthesis Repressor Proteins - metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae Proteins Sequence Deletion Transcription Factors - genetics Transcription, Genetic |
| title | The global transcriptional regulators, SSN6 and TUP1, play distinct roles in the establishment of a repressive chromatin structure |
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