Quantitative analysis of a polysulfated xylan (SP54) in urine using gas-liquid chromatography
A sensitive analytical method for the quantitation of a polysulfated xylan (SP54) in urine has been developed. SP54 and urinary glycosaminoglycans have been isolated from urine using cetylpyridinium chloride. This method removes all glycosaminoglycans with molecular weights less than 3000 Da. Follow...
Gespeichert in:
| Veröffentlicht in: | Analytical biochemistry 1986, Vol.152 (1), p.52-58 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 58 |
|---|---|
| container_issue | 1 |
| container_start_page | 52 |
| container_title | Analytical biochemistry |
| container_volume | 152 |
| creator | Lee, Christopher E.F. Edwards, Haydn E. Navaratnam, Suppiah Phyllips, Glyn O. |
| description | A sensitive analytical method for the quantitation of a polysulfated xylan (SP54) in urine has been developed. SP54 and urinary glycosaminoglycans have been isolated from urine using cetylpyridinium chloride. This method removes all glycosaminoglycans with molecular weights less than 3000 Da. Following isolation, SP54 and urinary glycosaminoglycans have been selectively hydrolyzed under conditions (0.5
m HCl/105°C/30 min) which produce an efficient yield of xylose from SP54 but not from the glycosaminoglycans. Xylose derived from SP54 has subsequently been determined using gas-liquid chromatography. Levels of SP54 down to 10 μg/ml have been determined using this technique. |
| doi_str_mv | 10.1016/0003-2697(86)90118-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_76749630</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0003269786901181</els_id><sourcerecordid>76749630</sourcerecordid><originalsourceid>FETCH-LOGICAL-c386t-8b9a63de8ac11dc71c7e98d92c0bf61a295252bbc6fe1f4e57c676c22023c0343</originalsourceid><addsrcrecordid>eNp9kE1r3DAQhkVpSTdp_0ELOpSQHJyOZFuWLoUS0g8INKXtsYjxeLxR8dobyQ7Zf1-7u-yxp2GYZ4Z3HiHeKLhSoMx7AMgzbVx1Yc2lA6Vspp6JlQJnMsjBPRerI_JSnKb0B2aoKM2JONGFBp3DSvz-PmE_hhHH8MgSe-x2KSQ5tBLldpibqWtx5EY-7Trs5cWPu7K4lKGXUww9yymFfi3XmLIuPEyhkXQfhw2Owzri9n73SrxosUv8-lDPxK9PNz-vv2S33z5_vf54m1FuzZjZ2qHJG7ZISjVUKarY2cZpgro1CrUrdanrmkzLqi24rMhUhvTyA0Fe5GfifH93G4eHidPoNyERd3NkHqbkK1MVzuQwg8UepDikFLn12xg2GHdegV-s-kWZX5R5a_w_q17Na28P96d6w81x6aBxnr87zDERdm3EnkI6Yha0tdUS88Me49nFY-DoEwXuiZsQmUbfDOH_Of4ClMqTQA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>76749630</pqid></control><display><type>article</type><title>Quantitative analysis of a polysulfated xylan (SP54) in urine using gas-liquid chromatography</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Lee, Christopher E.F. ; Edwards, Haydn E. ; Navaratnam, Suppiah ; Phyllips, Glyn O.</creator><creatorcontrib>Lee, Christopher E.F. ; Edwards, Haydn E. ; Navaratnam, Suppiah ; Phyllips, Glyn O.</creatorcontrib><description>A sensitive analytical method for the quantitation of a polysulfated xylan (SP54) in urine has been developed. SP54 and urinary glycosaminoglycans have been isolated from urine using cetylpyridinium chloride. This method removes all glycosaminoglycans with molecular weights less than 3000 Da. Following isolation, SP54 and urinary glycosaminoglycans have been selectively hydrolyzed under conditions (0.5
m HCl/105°C/30 min) which produce an efficient yield of xylose from SP54 but not from the glycosaminoglycans. Xylose derived from SP54 has subsequently been determined using gas-liquid chromatography. Levels of SP54 down to 10 μg/ml have been determined using this technique.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/0003-2697(86)90118-1</identifier><identifier>PMID: 2420230</identifier><identifier>CODEN: ANBCA2</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Applied sciences ; Chromatography, Gas ; Exact sciences and technology ; Gas Chromatography-Mass Spectrometry ; gel permeation chromatography ; glycosaminoglycans ; Glycosaminoglycans - urine ; Humans ; Hydrogen-Ion Concentration ; Hydrolysis ; Molecular Weight ; Other techniques and industries ; Pentosan Sulfuric Polyester - urine ; Polysaccharides - urine ; SP54 ; Xylose - urine</subject><ispartof>Analytical biochemistry, 1986, Vol.152 (1), p.52-58</ispartof><rights>1986</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-8b9a63de8ac11dc71c7e98d92c0bf61a295252bbc6fe1f4e57c676c22023c0343</citedby><cites>FETCH-LOGICAL-c386t-8b9a63de8ac11dc71c7e98d92c0bf61a295252bbc6fe1f4e57c676c22023c0343</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0003-2697(86)90118-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8028874$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2420230$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Christopher E.F.</creatorcontrib><creatorcontrib>Edwards, Haydn E.</creatorcontrib><creatorcontrib>Navaratnam, Suppiah</creatorcontrib><creatorcontrib>Phyllips, Glyn O.</creatorcontrib><title>Quantitative analysis of a polysulfated xylan (SP54) in urine using gas-liquid chromatography</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>A sensitive analytical method for the quantitation of a polysulfated xylan (SP54) in urine has been developed. SP54 and urinary glycosaminoglycans have been isolated from urine using cetylpyridinium chloride. This method removes all glycosaminoglycans with molecular weights less than 3000 Da. Following isolation, SP54 and urinary glycosaminoglycans have been selectively hydrolyzed under conditions (0.5
m HCl/105°C/30 min) which produce an efficient yield of xylose from SP54 but not from the glycosaminoglycans. Xylose derived from SP54 has subsequently been determined using gas-liquid chromatography. Levels of SP54 down to 10 μg/ml have been determined using this technique.</description><subject>Applied sciences</subject><subject>Chromatography, Gas</subject><subject>Exact sciences and technology</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>gel permeation chromatography</subject><subject>glycosaminoglycans</subject><subject>Glycosaminoglycans - urine</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Hydrolysis</subject><subject>Molecular Weight</subject><subject>Other techniques and industries</subject><subject>Pentosan Sulfuric Polyester - urine</subject><subject>Polysaccharides - urine</subject><subject>SP54</subject><subject>Xylose - urine</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1r3DAQhkVpSTdp_0ELOpSQHJyOZFuWLoUS0g8INKXtsYjxeLxR8dobyQ7Zf1-7u-yxp2GYZ4Z3HiHeKLhSoMx7AMgzbVx1Yc2lA6Vspp6JlQJnMsjBPRerI_JSnKb0B2aoKM2JONGFBp3DSvz-PmE_hhHH8MgSe-x2KSQ5tBLldpibqWtx5EY-7Trs5cWPu7K4lKGXUww9yymFfi3XmLIuPEyhkXQfhw2Owzri9n73SrxosUv8-lDPxK9PNz-vv2S33z5_vf54m1FuzZjZ2qHJG7ZISjVUKarY2cZpgro1CrUrdanrmkzLqi24rMhUhvTyA0Fe5GfifH93G4eHidPoNyERd3NkHqbkK1MVzuQwg8UepDikFLn12xg2GHdegV-s-kWZX5R5a_w_q17Na28P96d6w81x6aBxnr87zDERdm3EnkI6Yha0tdUS88Me49nFY-DoEwXuiZsQmUbfDOH_Of4ClMqTQA</recordid><startdate>1986</startdate><enddate>1986</enddate><creator>Lee, Christopher E.F.</creator><creator>Edwards, Haydn E.</creator><creator>Navaratnam, Suppiah</creator><creator>Phyllips, Glyn O.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1986</creationdate><title>Quantitative analysis of a polysulfated xylan (SP54) in urine using gas-liquid chromatography</title><author>Lee, Christopher E.F. ; Edwards, Haydn E. ; Navaratnam, Suppiah ; Phyllips, Glyn O.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-8b9a63de8ac11dc71c7e98d92c0bf61a295252bbc6fe1f4e57c676c22023c0343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Applied sciences</topic><topic>Chromatography, Gas</topic><topic>Exact sciences and technology</topic><topic>Gas Chromatography-Mass Spectrometry</topic><topic>gel permeation chromatography</topic><topic>glycosaminoglycans</topic><topic>Glycosaminoglycans - urine</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Hydrolysis</topic><topic>Molecular Weight</topic><topic>Other techniques and industries</topic><topic>Pentosan Sulfuric Polyester - urine</topic><topic>Polysaccharides - urine</topic><topic>SP54</topic><topic>Xylose - urine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Christopher E.F.</creatorcontrib><creatorcontrib>Edwards, Haydn E.</creatorcontrib><creatorcontrib>Navaratnam, Suppiah</creatorcontrib><creatorcontrib>Phyllips, Glyn O.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Christopher E.F.</au><au>Edwards, Haydn E.</au><au>Navaratnam, Suppiah</au><au>Phyllips, Glyn O.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitative analysis of a polysulfated xylan (SP54) in urine using gas-liquid chromatography</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1986</date><risdate>1986</risdate><volume>152</volume><issue>1</issue><spage>52</spage><epage>58</epage><pages>52-58</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><coden>ANBCA2</coden><abstract>A sensitive analytical method for the quantitation of a polysulfated xylan (SP54) in urine has been developed. SP54 and urinary glycosaminoglycans have been isolated from urine using cetylpyridinium chloride. This method removes all glycosaminoglycans with molecular weights less than 3000 Da. Following isolation, SP54 and urinary glycosaminoglycans have been selectively hydrolyzed under conditions (0.5
m HCl/105°C/30 min) which produce an efficient yield of xylose from SP54 but not from the glycosaminoglycans. Xylose derived from SP54 has subsequently been determined using gas-liquid chromatography. Levels of SP54 down to 10 μg/ml have been determined using this technique.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>2420230</pmid><doi>10.1016/0003-2697(86)90118-1</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2697 |
| ispartof | Analytical biochemistry, 1986, Vol.152 (1), p.52-58 |
| issn | 0003-2697 1096-0309 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76749630 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Applied sciences Chromatography, Gas Exact sciences and technology Gas Chromatography-Mass Spectrometry gel permeation chromatography glycosaminoglycans Glycosaminoglycans - urine Humans Hydrogen-Ion Concentration Hydrolysis Molecular Weight Other techniques and industries Pentosan Sulfuric Polyester - urine Polysaccharides - urine SP54 Xylose - urine |
| title | Quantitative analysis of a polysulfated xylan (SP54) in urine using gas-liquid chromatography |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T06%3A21%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Quantitative%20analysis%20of%20a%20polysulfated%20xylan%20(SP54)%20in%20urine%20using%20gas-liquid%20chromatography&rft.jtitle=Analytical%20biochemistry&rft.au=Lee,%20Christopher%20E.F.&rft.date=1986&rft.volume=152&rft.issue=1&rft.spage=52&rft.epage=58&rft.pages=52-58&rft.issn=0003-2697&rft.eissn=1096-0309&rft.coden=ANBCA2&rft_id=info:doi/10.1016/0003-2697(86)90118-1&rft_dat=%3Cproquest_cross%3E76749630%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=76749630&rft_id=info:pmid/2420230&rft_els_id=0003269786901181&rfr_iscdi=true |