The Drosophila orb RNA-binding protein is required for the formation of the egg chamber and establishment of polarity
The orb gene of Drosophila encodes sex-specific germ-line proteins that contain two RRM-type RNA-binding domains. Here we report the distribution of Orb protein in wild-type, tumorous, and orb mutant ovaries. The wild-type distribution of Orb protein during oogenesis resembles that of its RNA, prefe...
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| Veröffentlicht in: | Genes & development 1994-03, Vol.8 (5), p.598-613 |
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| creator | LANTZ, V CHANG, J. S HORABIN, J. I BOPP, D SCHEDL, P |
| description | The orb gene of Drosophila encodes sex-specific germ-line proteins that contain two RRM-type RNA-binding domains. Here we report the distribution of Orb protein in wild-type, tumorous, and orb mutant ovaries. The wild-type distribution of Orb protein during oogenesis resembles that of its RNA, preferentially accumulating in the cytoplasm of the developing oocyte shortly after the formation of the 16-cell cyst. As anticipated from its germ-line expression, mutations in orb lead to female sterility. Analysis of the effect of orb mutants on the distribution of RNAs known to be required for oocyte differentiation and polarity suggests that orb functions in RNA localization at multiple points during oogenesis. In addition, phenotypic characterization of the orb mutants indicates that the gene is required early in oogenesis for formation of the 16-cell cyst. It then functions in the differentiation of the oocyte and is required for the three-dimensional reorganization of the germ cells in the cyst as well as for the establishment of normal germ-line-soma interactions in the egg chamber. |
| doi_str_mv | 10.1101/gad.8.5.598 |
| format | Article |
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Analysis of the effect of orb mutants on the distribution of RNAs known to be required for oocyte differentiation and polarity suggests that orb functions in RNA localization at multiple points during oogenesis. In addition, phenotypic characterization of the orb mutants indicates that the gene is required early in oogenesis for formation of the 16-cell cyst. It then functions in the differentiation of the oocyte and is required for the three-dimensional reorganization of the germ cells in the cyst as well as for the establishment of normal germ-line-soma interactions in the egg chamber.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Classical genetics, quantitative genetics, hybrids</subject><subject>Drosophila</subject><subject>Drosophila - genetics</subject><subject>Drosophila Proteins</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. 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Biological and molecular evolution</subject><subject>Invertebrata</subject><subject>Male</subject><subject>Mutation</subject><subject>Oogenesis</subject><subject>Ovary - metabolism</subject><subject>Ovum - metabolism</subject><subject>RNA</subject><subject>RNA-Binding Proteins - physiology</subject><subject>Testis - metabolism</subject><issn>0890-9369</issn><issn>1549-5477</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1P3DAQxa0KBAvtqWckH1AvKNtx4o_4iKB8SKiVKjhbY8fZdZXEi50c-O9rYMWV00gzv3maN4-Q7wzWjAH7ucFu3a7FWuj2C1kxwXUluFIHZAWthko3Uh-Tk5z_AYAEKY_IkRJ1U3O-Isvj1tPrFHPcbcOANCZL__6-rGyYujBt6C7F2YeJhkyTf15C8h3tY6JzWSt1xDnEicb-reE3G-q2OFqfKE4d9XlGO4S8Hf00v0K7OGAK88tXctjjkP23fT0lTze_Hq_uqoc_t_dXlw-V41zNFZOt6IBLplqQrnHCggerlGg6Lm3tAHvROFv3WGvtEJwWHhV6WdvG171uTsmPd91i43kp55gxZOeHAScfl2yUVFwLCZ-C5ZJGqFoW8OIddOVnOfne7FIYMb0YBuY1DVPSMK0RpqRR6LO97GJH332w-_eX-fl-jtnh0CecXMgfGIeW6eL2P9EhkxI</recordid><startdate>19940301</startdate><enddate>19940301</enddate><creator>LANTZ, V</creator><creator>CHANG, J. 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Biological and molecular evolution</topic><topic>Invertebrata</topic><topic>Male</topic><topic>Mutation</topic><topic>Oogenesis</topic><topic>Ovary - metabolism</topic><topic>Ovum - metabolism</topic><topic>RNA</topic><topic>RNA-Binding Proteins - physiology</topic><topic>Testis - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LANTZ, V</creatorcontrib><creatorcontrib>CHANG, J. S</creatorcontrib><creatorcontrib>HORABIN, J. 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As anticipated from its germ-line expression, mutations in orb lead to female sterility. Analysis of the effect of orb mutants on the distribution of RNAs known to be required for oocyte differentiation and polarity suggests that orb functions in RNA localization at multiple points during oogenesis. In addition, phenotypic characterization of the orb mutants indicates that the gene is required early in oogenesis for formation of the 16-cell cyst. It then functions in the differentiation of the oocyte and is required for the three-dimensional reorganization of the germ cells in the cyst as well as for the establishment of normal germ-line-soma interactions in the egg chamber.</abstract><cop>Cold Spring Harbor, NY</cop><pub>Cold Spring Harbor Laboratory</pub><pmid>7523244</pmid><doi>10.1101/gad.8.5.598</doi><tpages>16</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | Genes & development, 1994-03, Vol.8 (5), p.598-613 |
| issn | 0890-9369 1549-5477 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Biological and medical sciences Blotting, Western Classical genetics, quantitative genetics, hybrids Drosophila Drosophila - genetics Drosophila Proteins Female Fundamental and applied biological sciences. Psychology Genes, Insect Genetics of eukaryotes. Biological and molecular evolution Invertebrata Male Mutation Oogenesis Ovary - metabolism Ovum - metabolism RNA RNA-Binding Proteins - physiology Testis - metabolism |
| title | The Drosophila orb RNA-binding protein is required for the formation of the egg chamber and establishment of polarity |
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