Inositol 1,4,5-trisphosphate-induced Ca2+ release from permeabilized mastocytoma cells

The effect of inositol 1,4,5-trisphosphate (IP3) on Ca2+ release in the transformed murine mast cells, mastocytoma P-815 cells permeabilized with digitonin was studied. Ca2+ was sequestered by intracellular organelles in the presence of ATP until the medium free Ca2+ concentration was lowered to a n...

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Veröffentlicht in:Biochemical and biophysical research communications 1986-02, Vol.135 (1), p.46-51
Hauptverfasser: Muto, Y, Tohmatsu, T, Yoshioka, S, Nozawa, Y
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container_title Biochemical and biophysical research communications
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creator Muto, Y
Tohmatsu, T
Yoshioka, S
Nozawa, Y
description The effect of inositol 1,4,5-trisphosphate (IP3) on Ca2+ release in the transformed murine mast cells, mastocytoma P-815 cells permeabilized with digitonin was studied. Ca2+ was sequestered by intracellular organelles in the presence of ATP until the medium free Ca2+ concentration was lowered to a new steady-state level. The subsequent addition of IP3 caused a rapid Ca2+ release, which was followed by a slow re-uptake of Ca2+. Fifty percent of the sequestered Ca2+ was released by 10 microM IP3. Maximal Ca2+ release occurred at 10 microM and half maximal activity was at 1.3 microM. These results indicate that IP3 may function as a messenger of intracellular Ca2+ mobilization in mastocytoma cells.
doi_str_mv 10.1016/0006-291X(86)90940-X
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subjects Adenosine Triphosphate - physiology
Aminoquinolines
Animals
Calcium - metabolism
Cell Compartmentation - drug effects
Cell Line
Cell Membrane Permeability - drug effects
Digitonin - pharmacology
Endoplasmic Reticulum - metabolism
Inositol Phosphates - pharmacology
Mast-Cell Sarcoma - metabolism
Mice
Sugar Phosphates - pharmacology
title Inositol 1,4,5-trisphosphate-induced Ca2+ release from permeabilized mastocytoma cells
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