Inositol 1,4,5-trisphosphate-induced Ca2+ release from permeabilized mastocytoma cells
The effect of inositol 1,4,5-trisphosphate (IP3) on Ca2+ release in the transformed murine mast cells, mastocytoma P-815 cells permeabilized with digitonin was studied. Ca2+ was sequestered by intracellular organelles in the presence of ATP until the medium free Ca2+ concentration was lowered to a n...
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Veröffentlicht in: | Biochemical and biophysical research communications 1986-02, Vol.135 (1), p.46-51 |
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creator | Muto, Y Tohmatsu, T Yoshioka, S Nozawa, Y |
description | The effect of inositol 1,4,5-trisphosphate (IP3) on Ca2+ release in the transformed murine mast cells, mastocytoma P-815 cells permeabilized with digitonin was studied. Ca2+ was sequestered by intracellular organelles in the presence of ATP until the medium free Ca2+ concentration was lowered to a new steady-state level. The subsequent addition of IP3 caused a rapid Ca2+ release, which was followed by a slow re-uptake of Ca2+. Fifty percent of the sequestered Ca2+ was released by 10 microM IP3. Maximal Ca2+ release occurred at 10 microM and half maximal activity was at 1.3 microM. These results indicate that IP3 may function as a messenger of intracellular Ca2+ mobilization in mastocytoma cells. |
doi_str_mv | 10.1016/0006-291X(86)90940-X |
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Ca2+ was sequestered by intracellular organelles in the presence of ATP until the medium free Ca2+ concentration was lowered to a new steady-state level. The subsequent addition of IP3 caused a rapid Ca2+ release, which was followed by a slow re-uptake of Ca2+. Fifty percent of the sequestered Ca2+ was released by 10 microM IP3. Maximal Ca2+ release occurred at 10 microM and half maximal activity was at 1.3 microM. 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Ca2+ was sequestered by intracellular organelles in the presence of ATP until the medium free Ca2+ concentration was lowered to a new steady-state level. The subsequent addition of IP3 caused a rapid Ca2+ release, which was followed by a slow re-uptake of Ca2+. Fifty percent of the sequestered Ca2+ was released by 10 microM IP3. Maximal Ca2+ release occurred at 10 microM and half maximal activity was at 1.3 microM. These results indicate that IP3 may function as a messenger of intracellular Ca2+ mobilization in mastocytoma cells.</description><subject>Adenosine Triphosphate - physiology</subject><subject>Aminoquinolines</subject><subject>Animals</subject><subject>Calcium - metabolism</subject><subject>Cell Compartmentation - drug effects</subject><subject>Cell Line</subject><subject>Cell Membrane Permeability - drug effects</subject><subject>Digitonin - pharmacology</subject><subject>Endoplasmic Reticulum - metabolism</subject><subject>Inositol Phosphates - pharmacology</subject><subject>Mast-Cell Sarcoma - metabolism</subject><subject>Mice</subject><subject>Sugar Phosphates - pharmacology</subject><issn>0006-291X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kMtOwzAQRb0AlfL4A5CyQiAaGDuJH0tU8ahUiQ2g7izbmYigpC52sihfT0KrLkYjzb13NHMIuaRwT4HyBwDgKVN0dSP5rQKVQ7o6ItPD-IScxvgNQGnO1YRMMpAsy2BKPhdrH-vONwmd5bMi7UIdN19-KNNhWq_L3mGZzA27SwI2aCImVfBtssHQorF1U_8Oemti5922861JHDZNPCfHlWkiXuz7Gfl4fnqfv6bLt5fF_HGZOporSEVhMiegUtZakTOhDONMqqwCdLaShtNSMiW4E-XwpUUoLAgsBolLZsFlZ-R6t3cT_E-PsdNtHccLzBp9H7XgIpcS6GDMd0YXfIwBK70JdWvCVlPQI0I9stIjKy25_keoV0Psar-_ty2Wh9CeX_YHP3NufA</recordid><startdate>19860226</startdate><enddate>19860226</enddate><creator>Muto, Y</creator><creator>Tohmatsu, T</creator><creator>Yoshioka, S</creator><creator>Nozawa, Y</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19860226</creationdate><title>Inositol 1,4,5-trisphosphate-induced Ca2+ release from permeabilized mastocytoma cells</title><author>Muto, Y ; Tohmatsu, T ; Yoshioka, S ; Nozawa, Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1490-75a3c70f9bbb74279a262893f0ecbf8a61d82976c7d016be05b07e5bf8682b0c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Adenosine Triphosphate - physiology</topic><topic>Aminoquinolines</topic><topic>Animals</topic><topic>Calcium - metabolism</topic><topic>Cell Compartmentation - drug effects</topic><topic>Cell Line</topic><topic>Cell Membrane Permeability - drug effects</topic><topic>Digitonin - pharmacology</topic><topic>Endoplasmic Reticulum - metabolism</topic><topic>Inositol Phosphates - pharmacology</topic><topic>Mast-Cell Sarcoma - metabolism</topic><topic>Mice</topic><topic>Sugar Phosphates - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Muto, Y</creatorcontrib><creatorcontrib>Tohmatsu, T</creatorcontrib><creatorcontrib>Yoshioka, S</creatorcontrib><creatorcontrib>Nozawa, Y</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Muto, Y</au><au>Tohmatsu, T</au><au>Yoshioka, S</au><au>Nozawa, Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inositol 1,4,5-trisphosphate-induced Ca2+ release from permeabilized mastocytoma cells</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1986-02-26</date><risdate>1986</risdate><volume>135</volume><issue>1</issue><spage>46</spage><epage>51</epage><pages>46-51</pages><issn>0006-291X</issn><abstract>The effect of inositol 1,4,5-trisphosphate (IP3) on Ca2+ release in the transformed murine mast cells, mastocytoma P-815 cells permeabilized with digitonin was studied. 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subjects | Adenosine Triphosphate - physiology Aminoquinolines Animals Calcium - metabolism Cell Compartmentation - drug effects Cell Line Cell Membrane Permeability - drug effects Digitonin - pharmacology Endoplasmic Reticulum - metabolism Inositol Phosphates - pharmacology Mast-Cell Sarcoma - metabolism Mice Sugar Phosphates - pharmacology |
title | Inositol 1,4,5-trisphosphate-induced Ca2+ release from permeabilized mastocytoma cells |
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