NMR solution structure of the recombinant tick anticoagulant protein (rTAP), a factor Xa inhibitor from the tick Ornithodoros moubata

The solution structure of the recombinant tick anticoagulant protein (rTAP) was determined by 1H nuclear magnetic resonance (NMR) spectroscopy in aqueous solution at pH 3.6 and 36°C. rTAP is a 60-residue protein functioning as a highly specific inhibitor of the coagulation protease factor Xa, which...

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Veröffentlicht in:	FEBS letters 1994-09, Vol.352 (2), p.251-257
Hauptverfasser:	Antuch, W., Güntert, P., Billeter, M., Hawthorne, T., Grossenbacher, H., Wüthrich, K.
Format:	Artikel
Sprache:	eng
Schlagworte:	2D, two-dimensional 2Q-spectroscopy, 2D two-quantum spectroscopy 2QF-COSY, 2D two-quantum-filtered correlation spectroscopy 3 J αβ, vicinal spin—spin coupling constant between the α-proton and a β-proton 3 JHNα, vicinal spin-spin coupling constant between the amide proton and the α-proton 3QF-COSY, 2D three-quantum-filtered correlation spectroscopy Acari Amino Acid Sequence Animals APPI, proteinase inhibitor domain of the Alzheimer's amyloid β-protein precursor Argasidae Arthropod Proteins Blood coagulation BPTI, bovine pancreatic trypsin inhibitor CD, circular dichroism E. COSY, 2D exclusive COSY Factor Xa Inhibitors Magnetic Resonance Spectroscopy Models, Molecular Molecular Sequence Data Molecular Structure NMR, nuclear magnetic resonance NOE, nuclear Overhauser effect NOESY, 2D NOE spectroscopy Nuclear magnetic resonance Ornithodoros moubata Peptides - chemistry Protein structure Protein Structure, Secondary Proteinase inhibitor Recombinant Fusion Proteins - chemistry rTAP, recombinant tick anticoagulant protein Sequence Alignment ShPI, Kunitz-type proteinase inhibitor from Stichodactyla helianthus TFA, trifluoroacetic acid Tick anticoagulant protein Ticks - chemistry TOCSY, 2D total correlation spectroscopy Toxin K, dendrotoxin K from Dendroaspis polylepis polylepis α-DTX, α-dendrotoxin from Dendroaspis angusticeps ΘMR, mean residue molar ellipticity
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