solid phase fluorescent immunoassay for the rapid detection of virus antigen or antibodies in fetuses infected with porcine parvovirus
A solid phase fluorescent immunoassay using polyacrylamide beads coated with rabbit anti-porcine parvovirus antibodies has been developed and utilized in the diagnosis of porcine parvovirus infection. The antibody-coated beads (immunobeads) were used both to detect virus in mummified fetal tissues a...
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| Veröffentlicht in: | Archives of virology 1986-01, Vol.88 (1/2), p.19-26 |
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| container_title | Archives of virology |
| container_volume | 88 |
| creator | Rivera, E Sjoland, L Karlsson, K.A |
| description | A solid phase fluorescent immunoassay using polyacrylamide beads coated with rabbit anti-porcine parvovirus antibodies has been developed and utilized in the diagnosis of porcine parvovirus infection. The antibody-coated beads (immunobeads) were used both to detect virus in mummified fetal tissues and to demonstrate specific antibodies in serum and ovarian follicular fluid. The immunobeads assay (IBA) was as sensitive as ELISA but more sensitive than virus isolation using tissue culture and haemagglutination tests. Both mummified and normal fetuses were obtained after experimental infection of SPF pregnant gilts. Using immunobeads, porcine parvovirus antigen was found in all mummified fetuses, but was found in only 1 out of 17 normal fetuses. |
| doi_str_mv | 10.1007/BF01310886 |
| format | Article |
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The antibody-coated beads (immunobeads) were used both to detect virus in mummified fetal tissues and to demonstrate specific antibodies in serum and ovarian follicular fluid. The immunobeads assay (IBA) was as sensitive as ELISA but more sensitive than virus isolation using tissue culture and haemagglutination tests. Both mummified and normal fetuses were obtained after experimental infection of SPF pregnant gilts. Using immunobeads, porcine parvovirus antigen was found in all mummified fetuses, but was found in only 1 out of 17 normal fetuses.</description><identifier>ISSN: 0304-8608</identifier><identifier>EISSN: 1432-8798</identifier><identifier>DOI: 10.1007/BF01310886</identifier><identifier>PMID: 3006641</identifier><language>eng</language><publisher>Wien: Springer</publisher><subject>Animals ; antibodies ; Antibodies, Viral - analysis ; antigens ; Antigens, Viral - analysis ; Biological and medical sciences ; Enzyme-Linked Immunosorbent Assay ; Female ; Fetal Diseases - immunology ; Fetal Diseases - veterinary ; fetus ; Fluorescent Antibody Technique ; Fundamental and applied biological sciences. Psychology ; Guinea Pigs ; Microbiology ; Parvoviridae - immunology ; Parvoviridae Infections - immunology ; Parvoviridae Infections - veterinary ; Porcine parvovirus ; Pregnancy ; Rabbits ; Swine ; Swine Diseases - diagnosis ; Swine Diseases - immunology ; Techniques used in virology ; Virology</subject><ispartof>Archives of virology, 1986-01, Vol.88 (1/2), p.19-26</ispartof><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c366t-3042ca9eac061d39c51b2dcc1de06c2e008b4a9f83a7d48a19c5fa864de8c08f3</citedby><cites>FETCH-LOGICAL-c366t-3042ca9eac061d39c51b2dcc1de06c2e008b4a9f83a7d48a19c5fa864de8c08f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8768444$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3006641$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rivera, E</creatorcontrib><creatorcontrib>Sjoland, L</creatorcontrib><creatorcontrib>Karlsson, K.A</creatorcontrib><title>solid phase fluorescent immunoassay for the rapid detection of virus antigen or antibodies in fetuses infected with porcine parvovirus</title><title>Archives of virology</title><addtitle>Arch Virol</addtitle><description>A solid phase fluorescent immunoassay using polyacrylamide beads coated with rabbit anti-porcine parvovirus antibodies has been developed and utilized in the diagnosis of porcine parvovirus infection. The antibody-coated beads (immunobeads) were used both to detect virus in mummified fetal tissues and to demonstrate specific antibodies in serum and ovarian follicular fluid. The immunobeads assay (IBA) was as sensitive as ELISA but more sensitive than virus isolation using tissue culture and haemagglutination tests. Both mummified and normal fetuses were obtained after experimental infection of SPF pregnant gilts. Using immunobeads, porcine parvovirus antigen was found in all mummified fetuses, but was found in only 1 out of 17 normal fetuses.</description><subject>Animals</subject><subject>antibodies</subject><subject>Antibodies, Viral - analysis</subject><subject>antigens</subject><subject>Antigens, Viral - analysis</subject><subject>Biological and medical sciences</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Fetal Diseases - immunology</subject><subject>Fetal Diseases - veterinary</subject><subject>fetus</subject><subject>Fluorescent Antibody Technique</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Guinea Pigs</subject><subject>Microbiology</subject><subject>Parvoviridae - immunology</subject><subject>Parvoviridae Infections - immunology</subject><subject>Parvoviridae Infections - veterinary</subject><subject>Porcine parvovirus</subject><subject>Pregnancy</subject><subject>Rabbits</subject><subject>Swine</subject><subject>Swine Diseases - diagnosis</subject><subject>Swine Diseases - immunology</subject><subject>Techniques used in virology</subject><subject>Virology</subject><issn>0304-8608</issn><issn>1432-8798</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhi0EKtuFC3eED4gDUmAcO45zhIoCUiUO0HM0a4-7Rkkc7KSoL8Bz4-6uypHTjGa--UczP2MvBLwTAO37j5cgpABj9CO2EUrWlWk785htQIKqjAbzlJ3n_BOgFGRzxs4kgNZKbNifHIfg-LzHTNwPa0yULU0LD-O4ThFzxjvuY-LLnnjCubCOFrJLiBOPnt-GtGaO0xJuqBTSId1FFyjzMHFPy5oPqS8z5PjvsOz5HJMNE_EZ0208KDxjTzwOmZ6f4pZdX376cfGluvr2-evFh6vKSq2XqpxTW-wILWjhZGcbsaudtcIRaFsTgNkp7LyR2DplUBTCo9HKkbFgvNyyN0fdOcVfK-WlH0O5dxhworjmvtWtaqBr_guWL3eglCzg2yNoU8w5ke_nFEZMd72A_t6d_p87BX55Ul13I7kH9GRH6b8-9TFbHHzCyYb8gJlWG1WWbtmrI-Yx9niTCnL9vS5bQKhGtqqVfwFdYKLd</recordid><startdate>19860101</startdate><enddate>19860101</enddate><creator>Rivera, E</creator><creator>Sjoland, L</creator><creator>Karlsson, K.A</creator><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19860101</creationdate><title>solid phase fluorescent immunoassay for the rapid detection of virus antigen or antibodies in fetuses infected with porcine parvovirus</title><author>Rivera, E ; Sjoland, L ; Karlsson, K.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c366t-3042ca9eac061d39c51b2dcc1de06c2e008b4a9f83a7d48a19c5fa864de8c08f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Animals</topic><topic>antibodies</topic><topic>Antibodies, Viral - analysis</topic><topic>antigens</topic><topic>Antigens, Viral - analysis</topic><topic>Biological and medical sciences</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>Fetal Diseases - immunology</topic><topic>Fetal Diseases - veterinary</topic><topic>fetus</topic><topic>Fluorescent Antibody Technique</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Guinea Pigs</topic><topic>Microbiology</topic><topic>Parvoviridae - immunology</topic><topic>Parvoviridae Infections - immunology</topic><topic>Parvoviridae Infections - veterinary</topic><topic>Porcine parvovirus</topic><topic>Pregnancy</topic><topic>Rabbits</topic><topic>Swine</topic><topic>Swine Diseases - diagnosis</topic><topic>Swine Diseases - immunology</topic><topic>Techniques used in virology</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rivera, E</creatorcontrib><creatorcontrib>Sjoland, L</creatorcontrib><creatorcontrib>Karlsson, K.A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rivera, E</au><au>Sjoland, L</au><au>Karlsson, K.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>solid phase fluorescent immunoassay for the rapid detection of virus antigen or antibodies in fetuses infected with porcine parvovirus</atitle><jtitle>Archives of virology</jtitle><addtitle>Arch Virol</addtitle><date>1986-01-01</date><risdate>1986</risdate><volume>88</volume><issue>1/2</issue><spage>19</spage><epage>26</epage><pages>19-26</pages><issn>0304-8608</issn><eissn>1432-8798</eissn><abstract>A solid phase fluorescent immunoassay using polyacrylamide beads coated with rabbit anti-porcine parvovirus antibodies has been developed and utilized in the diagnosis of porcine parvovirus infection. The antibody-coated beads (immunobeads) were used both to detect virus in mummified fetal tissues and to demonstrate specific antibodies in serum and ovarian follicular fluid. The immunobeads assay (IBA) was as sensitive as ELISA but more sensitive than virus isolation using tissue culture and haemagglutination tests. Both mummified and normal fetuses were obtained after experimental infection of SPF pregnant gilts. Using immunobeads, porcine parvovirus antigen was found in all mummified fetuses, but was found in only 1 out of 17 normal fetuses.</abstract><cop>Wien</cop><cop>New York, NY</cop><pub>Springer</pub><pmid>3006641</pmid><doi>10.1007/BF01310886</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0304-8608 |
| ispartof | Archives of virology, 1986-01, Vol.88 (1/2), p.19-26 |
| issn | 0304-8608 1432-8798 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76745095 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Animals antibodies Antibodies, Viral - analysis antigens Antigens, Viral - analysis Biological and medical sciences Enzyme-Linked Immunosorbent Assay Female Fetal Diseases - immunology Fetal Diseases - veterinary fetus Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Guinea Pigs Microbiology Parvoviridae - immunology Parvoviridae Infections - immunology Parvoviridae Infections - veterinary Porcine parvovirus Pregnancy Rabbits Swine Swine Diseases - diagnosis Swine Diseases - immunology Techniques used in virology Virology |
| title | solid phase fluorescent immunoassay for the rapid detection of virus antigen or antibodies in fetuses infected with porcine parvovirus |
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