Vaccination of sheep with purified serine proteases from the secretory and excretory material of Lucilia cuprina larvae
Sheep were vaccinated with two purified serine proteases, LCT25a and LCT25b, isolated from the secretory and excretory material from first instar larvae of Lucilia cuprina. The immunization produced a strong antibody response to LCT25b and a weaker response to LCT25a as measured by ELISA. However, n...
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| Veröffentlicht in: | International journal for parasitology 1994-08, Vol.24 (5), p.757-764 |
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| container_title | International journal for parasitology |
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| creator | Tellam, R.L. Eisemann, C.H. Pearson, R.D. |
| description | Sheep were vaccinated with two purified serine proteases, LCT25a and LCT25b, isolated from the secretory and excretory material from first instar larvae of
Lucilia cuprina. The immunization produced a strong antibody response to LCT25b and a weaker response to LCT25a as measured by ELISA. However, neither protease induced an ovine immune response which affected the development of first instar larvae growing on sera derived from these sheep. Further, direct
in vivo bioassays of larval growth on the backs of vaccinated sheep also indicated a lack of induction of an immune response which prevented establishment of the larvae. Sera from unvaccinated sheep which had previous experience of blowfly strike, in general, strongly recognised the serine protease LCT25b. It was concluded from all of these results that serine proteases from the secretory and excretory material of
L. cuprina are unlikely to be effective antigens in a vaccine designed to protect sheep from blowfly strike. |
| doi_str_mv | 10.1016/0020-7519(94)90132-5 |
| format | Article |
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Lucilia cuprina. The immunization produced a strong antibody response to LCT25b and a weaker response to LCT25a as measured by ELISA. However, neither protease induced an ovine immune response which affected the development of first instar larvae growing on sera derived from these sheep. Further, direct
in vivo bioassays of larval growth on the backs of vaccinated sheep also indicated a lack of induction of an immune response which prevented establishment of the larvae. Sera from unvaccinated sheep which had previous experience of blowfly strike, in general, strongly recognised the serine protease LCT25b. It was concluded from all of these results that serine proteases from the secretory and excretory material of
L. cuprina are unlikely to be effective antigens in a vaccine designed to protect sheep from blowfly strike.</description><identifier>ISSN: 0020-7519</identifier><identifier>EISSN: 1879-0135</identifier><identifier>DOI: 10.1016/0020-7519(94)90132-5</identifier><identifier>PMID: 7928080</identifier><identifier>CODEN: IJPYBT</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Animals ; Antibody Formation ; Biological and medical sciences ; Calliphoridae ; chymotrypsin ; Diptera ; Diptera - enzymology ; Diptera - immunology ; Infectious diseases ; Larva - enzymology ; Larva - immunology ; Lucilia cuprina ; Medical sciences ; Miscellaneous ; Myiasis - prevention & control ; Myiasis - veterinary ; Parasitic diseases ; secretory and excretory ; Serine Endopeptidases - immunology ; serine protease ; Sheep ; Sheep Diseases - prevention & control ; Vaccination - veterinary ; vaccine</subject><ispartof>International journal for parasitology, 1994-08, Vol.24 (5), p.757-764</ispartof><rights>1994</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-cff4df6269ea4965c08b1d750001f616fb090009fe0e794916ef5dda0ed3768f3</citedby><cites>FETCH-LOGICAL-c417t-cff4df6269ea4965c08b1d750001f616fb090009fe0e794916ef5dda0ed3768f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0020-7519(94)90132-5$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3552,27931,27932,46002</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4259758$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7928080$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tellam, R.L.</creatorcontrib><creatorcontrib>Eisemann, C.H.</creatorcontrib><creatorcontrib>Pearson, R.D.</creatorcontrib><title>Vaccination of sheep with purified serine proteases from the secretory and excretory material of Lucilia cuprina larvae</title><title>International journal for parasitology</title><addtitle>Int J Parasitol</addtitle><description>Sheep were vaccinated with two purified serine proteases, LCT25a and LCT25b, isolated from the secretory and excretory material from first instar larvae of
Lucilia cuprina. The immunization produced a strong antibody response to LCT25b and a weaker response to LCT25a as measured by ELISA. However, neither protease induced an ovine immune response which affected the development of first instar larvae growing on sera derived from these sheep. Further, direct
in vivo bioassays of larval growth on the backs of vaccinated sheep also indicated a lack of induction of an immune response which prevented establishment of the larvae. Sera from unvaccinated sheep which had previous experience of blowfly strike, in general, strongly recognised the serine protease LCT25b. It was concluded from all of these results that serine proteases from the secretory and excretory material of
L. cuprina are unlikely to be effective antigens in a vaccine designed to protect sheep from blowfly strike.</description><subject>Animals</subject><subject>Antibody Formation</subject><subject>Biological and medical sciences</subject><subject>Calliphoridae</subject><subject>chymotrypsin</subject><subject>Diptera</subject><subject>Diptera - enzymology</subject><subject>Diptera - immunology</subject><subject>Infectious diseases</subject><subject>Larva - enzymology</subject><subject>Larva - immunology</subject><subject>Lucilia cuprina</subject><subject>Medical sciences</subject><subject>Miscellaneous</subject><subject>Myiasis - prevention & control</subject><subject>Myiasis - veterinary</subject><subject>Parasitic diseases</subject><subject>secretory and excretory</subject><subject>Serine Endopeptidases - immunology</subject><subject>serine protease</subject><subject>Sheep</subject><subject>Sheep Diseases - prevention & control</subject><subject>Vaccination - veterinary</subject><subject>vaccine</subject><issn>0020-7519</issn><issn>1879-0135</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUuLFDEUhYMoY8_oP1DIQkQXpUlVHpWNIIOjQoMbdRtuJzd0pB5tkppx_r0pu-2lrpLL-c7hPgh5xtkbzrh6y1jLGi25eWXEa8N41zbyAdnwXpumVvIh2ZyRx-Qy5x-McdkJcUEutGl71rMNufsOzsUJSpwnOgea94gHehfLnh6WFENETzOmOCE9pLkgZMw0pHmkZY9VcQnLnO4pTJ7ir7_VCKV6YFgTt4uLQwTqlkONATpAugV8Qh4FGDI-Pb1X5NvNh6_Xn5rtl4-fr99vGye4Lo0LQfigWmUQhFHSsX7HvZasjhIUV2HHTP2bgAy1EYYrDNJ7YOg7rfrQXZGXx9za_c8Fc7FjzA6HASacl2y10kKYlv0X5EqpTvYrKI6gS3POCYOtc42Q7i1ndj2MXbdu161bI-yfw1hZbc9P-ctuRH82nS5R9RcnHbKDISSYXMxnTLTSaNlX7N0Rw7q024jJZhdxcuhjQlesn-O_-_gNHEqrIQ</recordid><startdate>19940801</startdate><enddate>19940801</enddate><creator>Tellam, R.L.</creator><creator>Eisemann, C.H.</creator><creator>Pearson, R.D.</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19940801</creationdate><title>Vaccination of sheep with purified serine proteases from the secretory and excretory material of Lucilia cuprina larvae</title><author>Tellam, R.L. ; Eisemann, C.H. ; Pearson, R.D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-cff4df6269ea4965c08b1d750001f616fb090009fe0e794916ef5dda0ed3768f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>Antibody Formation</topic><topic>Biological and medical sciences</topic><topic>Calliphoridae</topic><topic>chymotrypsin</topic><topic>Diptera</topic><topic>Diptera - enzymology</topic><topic>Diptera - immunology</topic><topic>Infectious diseases</topic><topic>Larva - enzymology</topic><topic>Larva - immunology</topic><topic>Lucilia cuprina</topic><topic>Medical sciences</topic><topic>Miscellaneous</topic><topic>Myiasis - prevention & control</topic><topic>Myiasis - veterinary</topic><topic>Parasitic diseases</topic><topic>secretory and excretory</topic><topic>Serine Endopeptidases - immunology</topic><topic>serine protease</topic><topic>Sheep</topic><topic>Sheep Diseases - prevention & control</topic><topic>Vaccination - veterinary</topic><topic>vaccine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tellam, R.L.</creatorcontrib><creatorcontrib>Eisemann, C.H.</creatorcontrib><creatorcontrib>Pearson, R.D.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>International journal for parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tellam, R.L.</au><au>Eisemann, C.H.</au><au>Pearson, R.D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Vaccination of sheep with purified serine proteases from the secretory and excretory material of Lucilia cuprina larvae</atitle><jtitle>International journal for parasitology</jtitle><addtitle>Int J Parasitol</addtitle><date>1994-08-01</date><risdate>1994</risdate><volume>24</volume><issue>5</issue><spage>757</spage><epage>764</epage><pages>757-764</pages><issn>0020-7519</issn><eissn>1879-0135</eissn><coden>IJPYBT</coden><abstract>Sheep were vaccinated with two purified serine proteases, LCT25a and LCT25b, isolated from the secretory and excretory material from first instar larvae of
Lucilia cuprina. The immunization produced a strong antibody response to LCT25b and a weaker response to LCT25a as measured by ELISA. However, neither protease induced an ovine immune response which affected the development of first instar larvae growing on sera derived from these sheep. Further, direct
in vivo bioassays of larval growth on the backs of vaccinated sheep also indicated a lack of induction of an immune response which prevented establishment of the larvae. Sera from unvaccinated sheep which had previous experience of blowfly strike, in general, strongly recognised the serine protease LCT25b. It was concluded from all of these results that serine proteases from the secretory and excretory material of
L. cuprina are unlikely to be effective antigens in a vaccine designed to protect sheep from blowfly strike.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>7928080</pmid><doi>10.1016/0020-7519(94)90132-5</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals Antibody Formation Biological and medical sciences Calliphoridae chymotrypsin Diptera Diptera - enzymology Diptera - immunology Infectious diseases Larva - enzymology Larva - immunology Lucilia cuprina Medical sciences Miscellaneous Myiasis - prevention & control Myiasis - veterinary Parasitic diseases secretory and excretory Serine Endopeptidases - immunology serine protease Sheep Sheep Diseases - prevention & control Vaccination - veterinary vaccine |
| title | Vaccination of sheep with purified serine proteases from the secretory and excretory material of Lucilia cuprina larvae |
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