The nude mouse xenograft system: A model for photodetection and photodynamic therapy in head and neck squamous cell carcinoma
Mechanisms for hematoporphyrin derivative (HPD) localization in malignant tissue and in photodynamic therapy (PDT) have not been established. The authors' experience with human cancer xenografts in nude mice as a tumor system for the experimental study of these mechanisms is described. Human mu...
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| Veröffentlicht in: | American journal of otolaryngology 1986, Vol.7 (1), p.17-27 |
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| container_title | American journal of otolaryngology |
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| creator | Hill, James H. Plant, Randall L. Harris, David M. Grossweiner, Leonard I. Rok, Bojan Seter, Andrew J. |
| description | Mechanisms for hematoporphyrin derivative (HPD) localization in malignant tissue and in photodynamic therapy (PDT) have not been established. The authors' experience with human cancer xenografts in nude mice as a tumor system for the experimental study of these mechanisms is described. Human mucosal head and neck squamous cell carcinoma was successfully transplanted to nude mice in 29 per cent of trials and serially passed through multiple generations in three tumor lines. Time required for progressive growth averaged 13.9 days, and 300 mm
3 tumors were obtained in four weeks. Take rates per passage varied, in part because of infection, and exponential growth rates varied among the three lines. However, within a single line exponential growth rates were similar. Average growth constants for the period of exponential growth were 0.14, 0.13, and 0.09 for the three lines. Fluorescent microscopy of excised xenografts revealed HPD fluorescence principally in connective tissue cells. Minimal fluorescence was seen in malignant epithelial cells. Spectrophotometric measures of HPD uptake in homogenized tissue showed highest values in liver. Tumor HPD levels were higher than those for either skin or muscle. The authors conclude that this tumor system can be used effectively to study HPD and PDT in head and neck cancer. |
| doi_str_mv | 10.1016/S0196-0709(86)80030-8 |
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3 tumors were obtained in four weeks. Take rates per passage varied, in part because of infection, and exponential growth rates varied among the three lines. However, within a single line exponential growth rates were similar. Average growth constants for the period of exponential growth were 0.14, 0.13, and 0.09 for the three lines. Fluorescent microscopy of excised xenografts revealed HPD fluorescence principally in connective tissue cells. Minimal fluorescence was seen in malignant epithelial cells. Spectrophotometric measures of HPD uptake in homogenized tissue showed highest values in liver. Tumor HPD levels were higher than those for either skin or muscle. The authors conclude that this tumor system can be used effectively to study HPD and PDT in head and neck cancer.</description><identifier>ISSN: 0196-0709</identifier><identifier>EISSN: 1532-818X</identifier><identifier>DOI: 10.1016/S0196-0709(86)80030-8</identifier><identifier>PMID: 3953966</identifier><identifier>CODEN: AJOTDP</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Animal tumors. Experimental tumors ; Animals ; Biological and medical sciences ; Carcinoma, Squamous Cell - pathology ; Disease Models, Animal ; Experimental head and neck tumors. Experimental orbital tumors ; Female ; Head and Neck Neoplasms - pathology ; Hematoporphyrins ; Humans ; Laryngeal Neoplasms - pathology ; Male ; Medical sciences ; Mice ; Mice, Nude ; Microscopy, Fluorescence ; Mouth Neoplasms - pathology ; Neoplasm Staging ; Neoplasm Transplantation ; Pharyngeal Neoplasms - pathology ; Photochemotherapy ; Transplantation, Heterologous ; Tumors</subject><ispartof>American journal of otolaryngology, 1986, Vol.7 (1), p.17-27</ispartof><rights>1986 Unknown</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-998d9b108422cd591c5fc6d750c3d4e1aa0aebeb64e712c49f004f922aedc9013</citedby><cites>FETCH-LOGICAL-c389t-998d9b108422cd591c5fc6d750c3d4e1aa0aebeb64e712c49f004f922aedc9013</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0196-0709(86)80030-8$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8691380$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3953966$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hill, James H.</creatorcontrib><creatorcontrib>Plant, Randall L.</creatorcontrib><creatorcontrib>Harris, David M.</creatorcontrib><creatorcontrib>Grossweiner, Leonard I.</creatorcontrib><creatorcontrib>Rok, Bojan</creatorcontrib><creatorcontrib>Seter, Andrew J.</creatorcontrib><title>The nude mouse xenograft system: A model for photodetection and photodynamic therapy in head and neck squamous cell carcinoma</title><title>American journal of otolaryngology</title><addtitle>Am J Otolaryngol</addtitle><description>Mechanisms for hematoporphyrin derivative (HPD) localization in malignant tissue and in photodynamic therapy (PDT) have not been established. The authors' experience with human cancer xenografts in nude mice as a tumor system for the experimental study of these mechanisms is described. Human mucosal head and neck squamous cell carcinoma was successfully transplanted to nude mice in 29 per cent of trials and serially passed through multiple generations in three tumor lines. Time required for progressive growth averaged 13.9 days, and 300 mm
3 tumors were obtained in four weeks. Take rates per passage varied, in part because of infection, and exponential growth rates varied among the three lines. However, within a single line exponential growth rates were similar. Average growth constants for the period of exponential growth were 0.14, 0.13, and 0.09 for the three lines. Fluorescent microscopy of excised xenografts revealed HPD fluorescence principally in connective tissue cells. Minimal fluorescence was seen in malignant epithelial cells. Spectrophotometric measures of HPD uptake in homogenized tissue showed highest values in liver. Tumor HPD levels were higher than those for either skin or muscle. The authors conclude that this tumor system can be used effectively to study HPD and PDT in head and neck cancer.</description><subject>Animal tumors. Experimental tumors</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Carcinoma, Squamous Cell - pathology</subject><subject>Disease Models, Animal</subject><subject>Experimental head and neck tumors. Experimental orbital tumors</subject><subject>Female</subject><subject>Head and Neck Neoplasms - pathology</subject><subject>Hematoporphyrins</subject><subject>Humans</subject><subject>Laryngeal Neoplasms - pathology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>Microscopy, Fluorescence</subject><subject>Mouth Neoplasms - pathology</subject><subject>Neoplasm Staging</subject><subject>Neoplasm Transplantation</subject><subject>Pharyngeal Neoplasms - pathology</subject><subject>Photochemotherapy</subject><subject>Transplantation, Heterologous</subject><subject>Tumors</subject><issn>0196-0709</issn><issn>1532-818X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1u1DAURi0EKkPhESp5gRAsAnacODYbVFX8VKrUBUViZ3mubxhDYk_tBDGLvjuemWi2XVn2d67vp0PIBWfvOePyw3fGtaxYx_RbJd8pxgSr1BOy4q2oK8XVz6dkdUKekxc5_2YFakR7Rs6EboWWckUe7jZIw-yQjnHOSP9hiL-S7Sead3nC8SO9LInDgfYx0e0mTuUyIUw-BmqDW552wY4e6LTBZLc76gPdoHUHICD8ofl-tvsFFHAYKNgEPsTRviTPejtkfLWc5-THl893V9-qm9uv11eXNxUIpadKa-X0mjPV1DW4VnNoe5CuaxkI1yC3lllc41o22PEaGt0z1vS6ri060IyLc_Lm-O82xfsZ82RGn_dVbMDSynSya2oh2gK2RxBSzDlhb7bJjzbtDGdmr90ctJu9U6OkOWg3qsxdLAvm9YjuNLV4LvnrJbcZ7NAnG8DnE6ak5kKxgn06Ylhk_PWYTAaPAdD5VJwbF_0jRf4DJnmhCA</recordid><startdate>1986</startdate><enddate>1986</enddate><creator>Hill, James H.</creator><creator>Plant, Randall L.</creator><creator>Harris, David M.</creator><creator>Grossweiner, Leonard I.</creator><creator>Rok, Bojan</creator><creator>Seter, Andrew J.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>8BM</scope></search><sort><creationdate>1986</creationdate><title>The nude mouse xenograft system: A model for photodetection and photodynamic therapy in head and neck squamous cell carcinoma</title><author>Hill, James H. ; Plant, Randall L. ; Harris, David M. ; Grossweiner, Leonard I. ; Rok, Bojan ; Seter, Andrew J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-998d9b108422cd591c5fc6d750c3d4e1aa0aebeb64e712c49f004f922aedc9013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Animal tumors. Experimental tumors</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Carcinoma, Squamous Cell - pathology</topic><topic>Disease Models, Animal</topic><topic>Experimental head and neck tumors. Experimental orbital tumors</topic><topic>Female</topic><topic>Head and Neck Neoplasms - pathology</topic><topic>Hematoporphyrins</topic><topic>Humans</topic><topic>Laryngeal Neoplasms - pathology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Nude</topic><topic>Microscopy, Fluorescence</topic><topic>Mouth Neoplasms - pathology</topic><topic>Neoplasm Staging</topic><topic>Neoplasm Transplantation</topic><topic>Pharyngeal Neoplasms - pathology</topic><topic>Photochemotherapy</topic><topic>Transplantation, Heterologous</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hill, James H.</creatorcontrib><creatorcontrib>Plant, Randall L.</creatorcontrib><creatorcontrib>Harris, David M.</creatorcontrib><creatorcontrib>Grossweiner, Leonard I.</creatorcontrib><creatorcontrib>Rok, Bojan</creatorcontrib><creatorcontrib>Seter, Andrew J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>ComDisDome</collection><jtitle>American journal of otolaryngology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hill, James H.</au><au>Plant, Randall L.</au><au>Harris, David M.</au><au>Grossweiner, Leonard I.</au><au>Rok, Bojan</au><au>Seter, Andrew J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The nude mouse xenograft system: A model for photodetection and photodynamic therapy in head and neck squamous cell carcinoma</atitle><jtitle>American journal of otolaryngology</jtitle><addtitle>Am J Otolaryngol</addtitle><date>1986</date><risdate>1986</risdate><volume>7</volume><issue>1</issue><spage>17</spage><epage>27</epage><pages>17-27</pages><issn>0196-0709</issn><eissn>1532-818X</eissn><coden>AJOTDP</coden><abstract>Mechanisms for hematoporphyrin derivative (HPD) localization in malignant tissue and in photodynamic therapy (PDT) have not been established. The authors' experience with human cancer xenografts in nude mice as a tumor system for the experimental study of these mechanisms is described. Human mucosal head and neck squamous cell carcinoma was successfully transplanted to nude mice in 29 per cent of trials and serially passed through multiple generations in three tumor lines. Time required for progressive growth averaged 13.9 days, and 300 mm
3 tumors were obtained in four weeks. Take rates per passage varied, in part because of infection, and exponential growth rates varied among the three lines. However, within a single line exponential growth rates were similar. Average growth constants for the period of exponential growth were 0.14, 0.13, and 0.09 for the three lines. Fluorescent microscopy of excised xenografts revealed HPD fluorescence principally in connective tissue cells. Minimal fluorescence was seen in malignant epithelial cells. Spectrophotometric measures of HPD uptake in homogenized tissue showed highest values in liver. Tumor HPD levels were higher than those for either skin or muscle. The authors conclude that this tumor system can be used effectively to study HPD and PDT in head and neck cancer.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>3953966</pmid><doi>10.1016/S0196-0709(86)80030-8</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0196-0709 |
| ispartof | American journal of otolaryngology, 1986, Vol.7 (1), p.17-27 |
| issn | 0196-0709 1532-818X |
| language | eng |
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| subjects | Animal tumors. Experimental tumors Animals Biological and medical sciences Carcinoma, Squamous Cell - pathology Disease Models, Animal Experimental head and neck tumors. Experimental orbital tumors Female Head and Neck Neoplasms - pathology Hematoporphyrins Humans Laryngeal Neoplasms - pathology Male Medical sciences Mice Mice, Nude Microscopy, Fluorescence Mouth Neoplasms - pathology Neoplasm Staging Neoplasm Transplantation Pharyngeal Neoplasms - pathology Photochemotherapy Transplantation, Heterologous Tumors |
| title | The nude mouse xenograft system: A model for photodetection and photodynamic therapy in head and neck squamous cell carcinoma |
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