Role of mesenchymal nidogen for epithelial morphogenesis in vitro
Recent biochemical studies suggested that the extracellular matrix protein nidogen is a binding molecule linking together basement membrane components. We studied its expression and role during development. By immunofluorescence and northern blotting, nidogen was found early during epithelial cell d...
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| Veröffentlicht in: | Development (Cambridge) 1994-07, Vol.120 (7), p.2003-2014 |
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| container_end_page | 2014 |
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| container_issue | 7 |
| container_start_page | 2003 |
| container_title | Development (Cambridge) |
| container_volume | 120 |
| creator | EKBLOM, P EKBLOM, M FECKER, L KLEIN, G HONG-YAN ZHANG KADOYA, Y MON-LI CHU MAYER, U TIMPL, R |
| description | Recent biochemical studies suggested that the extracellular matrix protein nidogen is a binding molecule linking together basement membrane components. We studied its expression and role during development. By immunofluorescence and northern blotting, nidogen was found early during epithelial cell development of kidney and lung. Yet, in situ hybridization revealed that nidogen was not produced by epithelium but by the adjacent mesenchyme in both organs. Binding of mesenchymal nidogen to epithelial laminin may thus be a key event during epithelial development. This is supported by antibody perturbation experiments. Antibodies against the nidogen binding site on laminin B2 chain perturbed epithelial development in vitro in embryonic kidney and lung. Mesenchymal nidogen could be important for early stages of epithelial morphogenesis. |
| doi_str_mv | 10.1242/dev.120.7.2003 |
| format | Article |
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We studied its expression and role during development. By immunofluorescence and northern blotting, nidogen was found early during epithelial cell development of kidney and lung. Yet, in situ hybridization revealed that nidogen was not produced by epithelium but by the adjacent mesenchyme in both organs. Binding of mesenchymal nidogen to epithelial laminin may thus be a key event during epithelial development. This is supported by antibody perturbation experiments. Antibodies against the nidogen binding site on laminin B2 chain perturbed epithelial development in vitro in embryonic kidney and lung. Mesenchymal nidogen could be important for early stages of epithelial morphogenesis.</description><identifier>ISSN: 0950-1991</identifier><identifier>EISSN: 1477-9129</identifier><identifier>DOI: 10.1242/dev.120.7.2003</identifier><identifier>PMID: 7925005</identifier><language>eng</language><publisher>Cambridge: The Company of Biologists Limited</publisher><subject>Animals ; Base Sequence ; Basement Membrane - physiology ; Biological and medical sciences ; Blotting, Northern ; Culture Techniques ; Embryology: invertebrates and vertebrates. Teratology ; Epithelium - chemistry ; Epithelium - physiology ; Fluorescent Antibody Technique ; Fundamental and applied biological sciences. Psychology ; In Situ Hybridization ; Kidney - chemistry ; Kidney - embryology ; Laminin - physiology ; Lung - chemistry ; Lung - embryology ; Membrane Glycoproteins - analysis ; Membrane Glycoproteins - physiology ; Mesoderm - physiology ; Mice ; Molecular Sequence Data ; Morphogenesis - physiology ; Oligonucleotide Probes ; Organogenesis. Fetal development ; Organogenesis. 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We studied its expression and role during development. By immunofluorescence and northern blotting, nidogen was found early during epithelial cell development of kidney and lung. Yet, in situ hybridization revealed that nidogen was not produced by epithelium but by the adjacent mesenchyme in both organs. Binding of mesenchymal nidogen to epithelial laminin may thus be a key event during epithelial development. This is supported by antibody perturbation experiments. Antibodies against the nidogen binding site on laminin B2 chain perturbed epithelial development in vitro in embryonic kidney and lung. Mesenchymal nidogen could be important for early stages of epithelial morphogenesis.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Basement Membrane - physiology</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Culture Techniques</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Epithelium - chemistry</subject><subject>Epithelium - physiology</subject><subject>Fluorescent Antibody Technique</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>In Situ Hybridization</subject><subject>Kidney - chemistry</subject><subject>Kidney - embryology</subject><subject>Laminin - physiology</subject><subject>Lung - chemistry</subject><subject>Lung - embryology</subject><subject>Membrane Glycoproteins - analysis</subject><subject>Membrane Glycoproteins - physiology</subject><subject>Mesoderm - physiology</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Morphogenesis - physiology</subject><subject>Oligonucleotide Probes</subject><subject>Organogenesis. Fetal development</subject><subject>Organogenesis. Physiological fonctions</subject><issn>0950-1991</issn><issn>1477-9129</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEFLJDEQhYMoOqtevQl9EG89pjpJp3MU2XUXBEH0HDLdlelIutOb9Lj47804g-zNUxX1vnpVPEIugC6h4tVNh2-5oUu5rChlB2QBXMpSQaUOyYIqQUtQCk7Ij5ReaSZqKY_JsVSVoFQsyO1T8FgEWwyYcGz798H4YnRdWONY2BALnNzco3d5PIQ49VsBk0uFG4s3N8dwRo6s8QnP9_WUvPz6-Xz3u3x4vP9zd_tQtlzwuVTWMEuBSYbWViBta20jOlMDo5Lx1gixMh3Nz3NBjUDWAQPTwcpUShnTsFNyvfOdYvi7wTTrwaUWvTcjhk3Sspas4cC-BaFuqARRZ3C5A9sYUopo9RTdYOK7Bqq34eocbm6olnobbl643DtvVgN2X_g-zaxf7XWTWuNtNGPr0hfGgTfwaVPusN6t-38uol654MPapTltT6IP0_9nPwAhQJHB</recordid><startdate>19940701</startdate><enddate>19940701</enddate><creator>EKBLOM, P</creator><creator>EKBLOM, M</creator><creator>FECKER, L</creator><creator>KLEIN, G</creator><creator>HONG-YAN ZHANG</creator><creator>KADOYA, Y</creator><creator>MON-LI CHU</creator><creator>MAYER, U</creator><creator>TIMPL, R</creator><general>The Company of Biologists Limited</general><general>Company of Biologists</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>19940701</creationdate><title>Role of mesenchymal nidogen for epithelial morphogenesis in vitro</title><author>EKBLOM, P ; EKBLOM, M ; FECKER, L ; KLEIN, G ; HONG-YAN ZHANG ; KADOYA, Y ; MON-LI CHU ; MAYER, U ; TIMPL, R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c454t-9fa3f01373eff217fcff85da6130734ca55bad0477450a5e3d131ad1ba299aa83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Basement Membrane - physiology</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Culture Techniques</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Epithelium - chemistry</topic><topic>Epithelium - physiology</topic><topic>Fluorescent Antibody Technique</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>In Situ Hybridization</topic><topic>Kidney - chemistry</topic><topic>Kidney - embryology</topic><topic>Laminin - physiology</topic><topic>Lung - chemistry</topic><topic>Lung - embryology</topic><topic>Membrane Glycoproteins - analysis</topic><topic>Membrane Glycoproteins - physiology</topic><topic>Mesoderm - physiology</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Morphogenesis - physiology</topic><topic>Oligonucleotide Probes</topic><topic>Organogenesis. Fetal development</topic><topic>Organogenesis. 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We studied its expression and role during development. By immunofluorescence and northern blotting, nidogen was found early during epithelial cell development of kidney and lung. Yet, in situ hybridization revealed that nidogen was not produced by epithelium but by the adjacent mesenchyme in both organs. Binding of mesenchymal nidogen to epithelial laminin may thus be a key event during epithelial development. This is supported by antibody perturbation experiments. Antibodies against the nidogen binding site on laminin B2 chain perturbed epithelial development in vitro in embryonic kidney and lung. Mesenchymal nidogen could be important for early stages of epithelial morphogenesis.</abstract><cop>Cambridge</cop><pub>The Company of Biologists Limited</pub><pmid>7925005</pmid><doi>10.1242/dev.120.7.2003</doi><tpages>12</tpages></addata></record> |
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| identifier | ISSN: 0950-1991 |
| ispartof | Development (Cambridge), 1994-07, Vol.120 (7), p.2003-2014 |
| issn | 0950-1991 1477-9129 |
| language | eng |
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| source | MEDLINE; The Company of Biologists; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | Animals Base Sequence Basement Membrane - physiology Biological and medical sciences Blotting, Northern Culture Techniques Embryology: invertebrates and vertebrates. Teratology Epithelium - chemistry Epithelium - physiology Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology In Situ Hybridization Kidney - chemistry Kidney - embryology Laminin - physiology Lung - chemistry Lung - embryology Membrane Glycoproteins - analysis Membrane Glycoproteins - physiology Mesoderm - physiology Mice Molecular Sequence Data Morphogenesis - physiology Oligonucleotide Probes Organogenesis. Fetal development Organogenesis. Physiological fonctions |
| title | Role of mesenchymal nidogen for epithelial morphogenesis in vitro |
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