Cocompartmentation of Proteins and K+ within the Living Cell

Monolayer H-50 tissue culture cells were treated with Triton X-100 and Brij 58 nonionic detergents, and their electron microscopic morphology along with the release of the intracellular proteins and K+ were studied. Although Triton X-100 was more effective, both detergents removed the lipoid membran...

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Veröffentlicht in:	Proc. Natl. Acad. Sci. U.S.A.; (United States) 1986-02, Vol.83 (4), p.1011-1015
Hauptverfasser:	Kellermayer, Miklos, Ludany, Andrea, Jobst, Kazmer, Szucs, Gyorgy, Trombitas, Karoly, Hazlewood, Carlton F.
Format:	Artikel
Sprache:	eng
Schlagworte:	550301 - Cytology- Tracer Techniques ADDITIVES ALKALI METAL ISOTOPES AMINO ACIDS Animals BASIC BIOLOGICAL SCIENCES BETA DECAY RADIOISOTOPES BETA-MINUS DECAY RADIOISOTOPES BIOCHEMICAL REACTION KINETICS Biological and medical sciences CARBOXYLIC ACIDS CATIONS Cell biology Cell Compartmentation CELL CULTURES Cell Line Cell Membrane - analysis Cell Membrane - drug effects Cell membranes Cell metabolism, cell oxidation Cell physiology Cells Cercopithecus aethiops Cetomacrogol CHARGED PARTICLES COMPARTMENTS Cultured cells Cytoplasm Cytoskeleton DAYS LIVING RADIOISOTOPES DETERGENTS DIFFUSION DRUGS Electrons Electrophoresis, Polyacrylamide Gel EMULSIFIERS EVEN-ODD NUCLEI Fundamental and applied biological sciences. Psychology Gels HOURS LIVING RADIOISOTOPES INTERMEDIATE MASS NUCLEI IONS ISOTOPES KINETICS LABELLED COMPOUNDS LIGHT NUCLEI LIPOTROPIC FACTORS MEMBRANE TRANSPORT METHIONINE MICROORGANISMS Microscopy, Electron Molecular and cellular biology NUCLEI Octoxynol ODD-ODD NUCLEI ORGANIC ACIDS ORGANIC COMPOUNDS ORGANIC SULFUR COMPOUNDS PARASITES Polyethylene Glycols Potassium - isolation & purification Potassium - metabolism POTASSIUM 42 POTASSIUM ISOTOPES Proteins - isolation & purification Proteins - metabolism RADIOISOTOPES REACTION KINETICS SIMIAN VIRUS Solubility SULFUR 35 SULFUR ISOTOPES SURFACTANTS Tritons VIRUSES WETTING AGENTS
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