Pharmacological evaluation of the isolated rat seminal vesicle preparation

A modification of the rat isolated seminal vesicle preparation is described, emphasizing the necessity to use younger animals (40–50 days old and weighing between 125 and 150 g) and to expel thoroughly all vesicular contents. Under the experimental conditions used (tissues suspended under a resting...

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Veröffentlicht in:Journal of pharmacological methods 1986-02, Vol.15 (1), p.65-75
Hauptverfasser: Sharif, S.I., Gokhale, S.D.
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description A modification of the rat isolated seminal vesicle preparation is described, emphasizing the necessity to use younger animals (40–50 days old and weighing between 125 and 150 g) and to expel thoroughly all vesicular contents. Under the experimental conditions used (tissues suspended under a resting tension of 350 mg in a continuous flow of a modified Krebs solution run at the rate of 15 ml min , maintained at 32°C, and bubbled with 5% CO 2 in O 2, the preparation was quite sensitive, but only to a few selected agonists, and remained viable for over 4–6 hr. Adrenaline, noradrenaline, dopamine, and acetylcholine all produced concentration-dependent and reproducible contractions. However, histaminergic, serotoninergic, purinergic, and opioid agonists were inactive as were prostaglandins of the E and F series and the polypeptides angiotensin, vasopressin, and oxytocin. In general, the tissue was rather insensitive to relaxant drugs, with only papaverine and sodium nitrite producing some relaxation in tissues previously contracted by carbachol. Advantages of the preparation include marked responsiveness, but only to a few selected agonists, and suitability for use as a paired tissue. It is suggested that employed under suitable experimental conditions, the preparation deserves a more frequent consideration for use during pharmacological investigations concerned with postsynaptic aspects of noradrenergic or cholinergic transmission.
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Under the experimental conditions used (tissues suspended under a resting tension of 350 mg in a continuous flow of a modified Krebs solution run at the rate of 15 ml min , maintained at 32°C, and bubbled with 5% CO 2 in O 2, the preparation was quite sensitive, but only to a few selected agonists, and remained viable for over 4–6 hr. Adrenaline, noradrenaline, dopamine, and acetylcholine all produced concentration-dependent and reproducible contractions. However, histaminergic, serotoninergic, purinergic, and opioid agonists were inactive as were prostaglandins of the E and F series and the polypeptides angiotensin, vasopressin, and oxytocin. In general, the tissue was rather insensitive to relaxant drugs, with only papaverine and sodium nitrite producing some relaxation in tissues previously contracted by carbachol. Advantages of the preparation include marked responsiveness, but only to a few selected agonists, and suitability for use as a paired tissue. 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Drug treatments</topic><topic>Rat seminal vesicle</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Reserpine - pharmacology</topic><topic>Seminal Vesicles - drug effects</topic><topic>Tyramine - pharmacology</topic><toplevel>online_resources</toplevel><creatorcontrib>Sharif, S.I.</creatorcontrib><creatorcontrib>Gokhale, S.D.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmacological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sharif, S.I.</au><au>Gokhale, S.D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pharmacological evaluation of the isolated rat seminal vesicle preparation</atitle><jtitle>Journal of pharmacological methods</jtitle><addtitle>J Pharmacol Methods</addtitle><date>1986-02-01</date><risdate>1986</risdate><volume>15</volume><issue>1</issue><spage>65</spage><epage>75</epage><pages>65-75</pages><issn>0160-5402</issn><coden>JPMED9</coden><abstract>A modification of the rat isolated seminal vesicle preparation is described, emphasizing the necessity to use younger animals (40–50 days old and weighing between 125 and 150 g) and to expel thoroughly all vesicular contents. Under the experimental conditions used (tissues suspended under a resting tension of 350 mg in a continuous flow of a modified Krebs solution run at the rate of 15 ml min , maintained at 32°C, and bubbled with 5% CO 2 in O 2, the preparation was quite sensitive, but only to a few selected agonists, and remained viable for over 4–6 hr. Adrenaline, noradrenaline, dopamine, and acetylcholine all produced concentration-dependent and reproducible contractions. However, histaminergic, serotoninergic, purinergic, and opioid agonists were inactive as were prostaglandins of the E and F series and the polypeptides angiotensin, vasopressin, and oxytocin. In general, the tissue was rather insensitive to relaxant drugs, with only papaverine and sodium nitrite producing some relaxation in tissues previously contracted by carbachol. Advantages of the preparation include marked responsiveness, but only to a few selected agonists, and suitability for use as a paired tissue. It is suggested that employed under suitable experimental conditions, the preparation deserves a more frequent consideration for use during pharmacological investigations concerned with postsynaptic aspects of noradrenergic or cholinergic transmission.</abstract><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>3951238</pmid><doi>10.1016/0160-5402(86)90006-9</doi><tpages>11</tpages></addata></record>
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subjects Acetylcholine
Acetylcholine - pharmacology
Adrenaline
Animals
Biological and medical sciences
Bradykinin - pharmacology
Cocaine - pharmacology
Dopamine
Dopamine - pharmacology
Epinephrine - pharmacology
Genital system. Reproduction
In Vitro Techniques
Male
Medical sciences
Muscle Contraction - drug effects
Muscle, Smooth - drug effects
Noradrenaline
Norepinephrine - pharmacology
Pharmacology. Drug treatments
Rat seminal vesicle
Rats
Rats, Inbred Strains
Reserpine - pharmacology
Seminal Vesicles - drug effects
Tyramine - pharmacology
title Pharmacological evaluation of the isolated rat seminal vesicle preparation
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