New methodology for assessment of the Langerhans cell network
A new procedure is described for staining Langerhans cells (LCs) based on the ability of anti‐S‐100 antibody to stain both epidermal LCs and melanocytes, while L‐Dopa stains only melanocytes. This procedure can be used on paraffin‐embedded skin sections and is therefore advantageous for examination...
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| Veröffentlicht in: | The Journal of pathology 1986-02, Vol.148 (2), p.127-134 |
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| container_title | The Journal of pathology |
| container_volume | 148 |
| creator | Halliday, Gary M. McArdle, John P. Knight, Brett A. Muller, H. Konrad |
| description | A new procedure is described for staining Langerhans cells (LCs) based on the ability of anti‐S‐100 antibody to stain both epidermal LCs and melanocytes, while L‐Dopa stains only melanocytes. This procedure can be used on paraffin‐embedded skin sections and is therefore advantageous for examination of pathological skin specimens. In order to determine how best to quantitate LCs in skin sections the distribution of LCs has been investigated using an improved method for preparation of epidermal sheets from mouse skin. Epidermal LCs stained for their surface membrane‐bound enzyme adenosine triphosphatase were observed to link with each other via their dendrites, forming a single cell layer which undulates throughout the epidermis. It is therefore proposed that LCs in skin sections should be enumerated per unit length, after identification in paraffin‐embedded sections double stained with anti‐S‐100 antibody and L‐Dopa. |
| doi_str_mv | 10.1002/path.1711480202 |
| format | Article |
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It is therefore proposed that LCs in skin sections should be enumerated per unit length, after identification in paraffin‐embedded sections double stained with anti‐S‐100 antibody and L‐Dopa.</description><subject>Adenosine Triphosphatases - metabolism</subject><subject>Animals</subject><subject>ATPase</subject><subject>Cell Count</subject><subject>Cytological Techniques</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>L-Dopa</subject><subject>Langerhans cells</subject><subject>Langerhans Cells - cytology</subject><subject>Langerhans Cells - enzymology</subject><subject>Levodopa</subject><subject>Melanocytes - cytology</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>S-100</subject><subject>S100 Proteins</subject><subject>Skin - cytology</subject><subject>skin pathology</subject><subject>Staining and Labeling</subject><issn>0022-3417</issn><issn>1096-9896</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtPAjEUhRujQUTXrkxm5W6gj3k1xgUhAhqCGDG6azqdW0BmptgOQf69QyAYV67u4n7n5ORD6JrgNsGYdlaymrdJTEiQYIrpCWoSzCOfJzw6Rc2aoD4LSHyOLpz7xBhzHoYN1KAB4SHlTXQ_ho1XQDU3mcnNbOtpYz3pHDhXQFl5RnvVHLyRLGdg57J0noI890qoNsYuL9GZlrmDq8Ntobf-w7Q39EfPg8ded-QrltQLSKxUoqNEs4hEWsZK8xAY5xwIzVJNAsniFICSkEmVyiTFimWKas6DJNIsYy10u-9dWfO1BleJYuF2Q2QJZu1EHMWMcUpqsLMHlTXOWdBiZReFtFtBsNgJEzth4ldYnbg5VK_TArIjfzBU_-_2_80ih-1_dWLSnQ7_tPv79MJV8H1MS7sU9eQ4FO_jgWAvk4_-02siCPsBzH-Hrw</recordid><startdate>198602</startdate><enddate>198602</enddate><creator>Halliday, Gary M.</creator><creator>McArdle, John P.</creator><creator>Knight, Brett A.</creator><creator>Muller, H. 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Konrad</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3822-17cc8f68f3616fa7cf95e3999e12dbf14a37bee2153acba8b0c3dc2f99486f3d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Adenosine Triphosphatases - metabolism</topic><topic>Animals</topic><topic>ATPase</topic><topic>Cell Count</topic><topic>Cytological Techniques</topic><topic>Humans</topic><topic>Immunoenzyme Techniques</topic><topic>L-Dopa</topic><topic>Langerhans cells</topic><topic>Langerhans Cells - cytology</topic><topic>Langerhans Cells - enzymology</topic><topic>Levodopa</topic><topic>Melanocytes - cytology</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>S-100</topic><topic>S100 Proteins</topic><topic>Skin - cytology</topic><topic>skin pathology</topic><topic>Staining and Labeling</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Halliday, Gary M.</creatorcontrib><creatorcontrib>McArdle, John P.</creatorcontrib><creatorcontrib>Knight, Brett A.</creatorcontrib><creatorcontrib>Muller, H. Konrad</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Halliday, Gary M.</au><au>McArdle, John P.</au><au>Knight, Brett A.</au><au>Muller, H. Konrad</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>New methodology for assessment of the Langerhans cell network</atitle><jtitle>The Journal of pathology</jtitle><addtitle>J. Pathol</addtitle><date>1986-02</date><risdate>1986</risdate><volume>148</volume><issue>2</issue><spage>127</spage><epage>134</epage><pages>127-134</pages><issn>0022-3417</issn><eissn>1096-9896</eissn><abstract>A new procedure is described for staining Langerhans cells (LCs) based on the ability of anti‐S‐100 antibody to stain both epidermal LCs and melanocytes, while L‐Dopa stains only melanocytes. This procedure can be used on paraffin‐embedded skin sections and is therefore advantageous for examination of pathological skin specimens. In order to determine how best to quantitate LCs in skin sections the distribution of LCs has been investigated using an improved method for preparation of epidermal sheets from mouse skin. Epidermal LCs stained for their surface membrane‐bound enzyme adenosine triphosphatase were observed to link with each other via their dendrites, forming a single cell layer which undulates throughout the epidermis. It is therefore proposed that LCs in skin sections should be enumerated per unit length, after identification in paraffin‐embedded sections double stained with anti‐S‐100 antibody and L‐Dopa.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>2419529</pmid><doi>10.1002/path.1711480202</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-3417 |
| ispartof | The Journal of pathology, 1986-02, Vol.148 (2), p.127-134 |
| issn | 0022-3417 1096-9896 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76733921 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Adenosine Triphosphatases - metabolism Animals ATPase Cell Count Cytological Techniques Humans Immunoenzyme Techniques L-Dopa Langerhans cells Langerhans Cells - cytology Langerhans Cells - enzymology Levodopa Melanocytes - cytology Mice Mice, Inbred C57BL S-100 S100 Proteins Skin - cytology skin pathology Staining and Labeling |
| title | New methodology for assessment of the Langerhans cell network |
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