Functional Immunoliposomes Harboring a Biosynthetically Lipid-Tagged Single-Chain Antibody

An anti-2-phenyloxazolone single-chain antibody was expressed in Escherichia coli as a lipoprotein fusion in order to generate a biosynthetically lipid-tagged molecule [Laukkanen et al. (1993) Protein Eng. 6, 449-454]. For purification, a hexahistidinyl tag was introduced to the C-terminus of the pr...

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Veröffentlicht in:	Biochemistry (Easton) 1994-09, Vol.33 (38), p.11664-11670
Hauptverfasser:	Laukkanen, Marja-Leena, Alfthan, Kaija, Keinanen, Kari
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies - chemistry Antibodies - genetics Base Sequence Drug Delivery Systems Escherichia coli - genetics Haptens - immunology Lipoproteins - chemistry Lipoproteins - genetics Liposomes - chemistry Molecular Sequence Data Oxazolone - analogs & derivatives Oxazolone - immunology Protein Engineering Recombinant Fusion Proteins - chemistry
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