An in Vitro Investigation of the Intracellular Bioactivity of Amoxicillin, Clindamycin, and Erythromycin for Staphylococcus aureus
The intraphagocytic bioactivities for Staphylococcus aureus of amoxicillin, clindamycin, and erythromycin (0.0075-20 µ/ml) were measured in human polymorphonuclear leukocytes (PMNLs) with the combination of a fluorochrome microassay and a radioassay. PMNLs with normal or depleted membrane-associated...
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| Veröffentlicht in: | The Journal of infectious diseases 1986-03, Vol.153 (3), p.593-600 |
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| container_title | The Journal of infectious diseases |
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| creator | Anderson, R. Joone, G. van Rensburg, C. E. J. |
| description | The intraphagocytic bioactivities for Staphylococcus aureus of amoxicillin, clindamycin, and erythromycin (0.0075-20 µ/ml) were measured in human polymorphonuclear leukocytes (PMNLs) with the combination of a fluorochrome microassay and a radioassay. PMNLs with normal or depleted membrane-associated oxidative metabolism were used to investigate the interactions that may occur between the intrinsic O2 dependent antimicrobial systems of human phagocytes and antimicrobial agents in the elimination of intracellular microbial pathogens. Neutralization of 02-dependent antimicrobial systems with retention of phagocytic capacity was achieved with use of PMNLs.from four children with chronic granulomatous disease (COD) or NaF-pulsed normalPMNLs. None of the test antibiotics possessed intracellular bactericidal activity. Clindamycin and erythromycin possessed significant intracellular bacteriostatic activity relativ~ to the modest activity of amoxicillin. Optimal intracellular bioactivity of all three antibiotics was obtained with normal PMNLs relative to NaF-pulsed or COD PMNLs, a result indicating the existence of beneficial interactions between the antimicrobial agents and the O2 dependent antimicrobial systems of PMNLs. |
| doi_str_mv | 10.1093/infdis/153.3.593 |
| format | Article |
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E. J.</creator><creatorcontrib>Anderson, R. ; Joone, G. ; van Rensburg, C. E. J.</creatorcontrib><description>The intraphagocytic bioactivities for Staphylococcus aureus of amoxicillin, clindamycin, and erythromycin (0.0075-20 µ/ml) were measured in human polymorphonuclear leukocytes (PMNLs) with the combination of a fluorochrome microassay and a radioassay. PMNLs with normal or depleted membrane-associated oxidative metabolism were used to investigate the interactions that may occur between the intrinsic O2 dependent antimicrobial systems of human phagocytes and antimicrobial agents in the elimination of intracellular microbial pathogens. Neutralization of 02-dependent antimicrobial systems with retention of phagocytic capacity was achieved with use of PMNLs.from four children with chronic granulomatous disease (COD) or NaF-pulsed normalPMNLs. None of the test antibiotics possessed intracellular bactericidal activity. Clindamycin and erythromycin possessed significant intracellular bacteriostatic activity relativ~ to the modest activity of amoxicillin. Optimal intracellular bioactivity of all three antibiotics was obtained with normal PMNLs relative to NaF-pulsed or COD PMNLs, a result indicating the existence of beneficial interactions between the antimicrobial agents and the O2 dependent antimicrobial systems of PMNLs.</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1093/infdis/153.3.593</identifier><identifier>PMID: 3950443</identifier><identifier>CODEN: JIDIAQ</identifier><language>eng</language><publisher>Chicago, IL: Oxford University Press</publisher><subject>Amoxicillin - pharmacology ; Antibacterial agents ; Antibiotics ; Antibiotics. Antiinfectious agents. Antiparasitic agents ; Antimicrobials ; Bacteria ; Biological and medical sciences ; Chemiluminescence ; Chronic granulomatous disease ; Clindamycin - pharmacology ; Erythromycin - pharmacology ; Fluorescence ; Fluorometry ; Humans ; Medical sciences ; Methods ; Microbial Sensitivity Tests ; Neutrophils - drug effects ; Neutrophils - microbiology ; Original Articles ; Pathogens ; Phagocytes ; Phagocytosis ; Pharmacology. 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E. J.</creatorcontrib><title>An in Vitro Investigation of the Intracellular Bioactivity of Amoxicillin, Clindamycin, and Erythromycin for Staphylococcus aureus</title><title>The Journal of infectious diseases</title><addtitle>J Infect Dis</addtitle><description>The intraphagocytic bioactivities for Staphylococcus aureus of amoxicillin, clindamycin, and erythromycin (0.0075-20 µ/ml) were measured in human polymorphonuclear leukocytes (PMNLs) with the combination of a fluorochrome microassay and a radioassay. PMNLs with normal or depleted membrane-associated oxidative metabolism were used to investigate the interactions that may occur between the intrinsic O2 dependent antimicrobial systems of human phagocytes and antimicrobial agents in the elimination of intracellular microbial pathogens. Neutralization of 02-dependent antimicrobial systems with retention of phagocytic capacity was achieved with use of PMNLs.from four children with chronic granulomatous disease (COD) or NaF-pulsed normalPMNLs. None of the test antibiotics possessed intracellular bactericidal activity. Clindamycin and erythromycin possessed significant intracellular bacteriostatic activity relativ~ to the modest activity of amoxicillin. Optimal intracellular bioactivity of all three antibiotics was obtained with normal PMNLs relative to NaF-pulsed or COD PMNLs, a result indicating the existence of beneficial interactions between the antimicrobial agents and the O2 dependent antimicrobial systems of PMNLs.</description><subject>Amoxicillin - pharmacology</subject><subject>Antibacterial agents</subject><subject>Antibiotics</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Antimicrobials</subject><subject>Bacteria</subject><subject>Biological and medical sciences</subject><subject>Chemiluminescence</subject><subject>Chronic granulomatous disease</subject><subject>Clindamycin - pharmacology</subject><subject>Erythromycin - pharmacology</subject><subject>Fluorescence</subject><subject>Fluorometry</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Methods</subject><subject>Microbial Sensitivity Tests</subject><subject>Neutrophils - drug effects</subject><subject>Neutrophils - microbiology</subject><subject>Original Articles</subject><subject>Pathogens</subject><subject>Phagocytes</subject><subject>Phagocytosis</subject><subject>Pharmacology. Drug treatments</subject><subject>Sodium Fluoride - pharmacology</subject><subject>Staphylococcus aureus - drug effects</subject><subject>Viability</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUc9vFCEYJUZT1-rdiwkH48nZwjAMw3HddrdNmnioNsYLYfnhUmdgBabpXP3LZd21PZoQ-Pje-154PADeYjTHiJMz56126QxTMidzyskzMCs1q9oWk-dghlBdV7jj_CV4ldIdQqghLTsBJ4RT1DRkBn4vPHQe3rocA7zy9yZl90NmFzwMFuatKc0cpTJ9P_Yywk8uSJXdvcvTnrAYwoNTru-d_wiXZddymNT-Ir2GF3HK2xj-dqANEd5kudtOfVBBqTFBOUYzptfghZV9Mm-O5yn4urr4srysrj-vr5aL60qRmueqprxjjTWM6_Ic1XBMNDWyYWbDy5JcI7IxuttQyjrUWqMZJY2hqO64MtiSU_DhoLuL4ddYjIrBpb0x6U0Yk2AtI6ir0X-JuHwippgXIjoQVQwpRWPFLrpBxklgJPb5iEM-omQiiCj5lJF3R-1xMxj9OHAMpODvj7hMSvY2Sq-KwD9ax9qOcv4kc5dyiE8qCCPa8rbg1QF3KZuHR1zGn6K4ZFRcfvsu1vXt6mZ1fi7W5A-WBbV0</recordid><startdate>198603</startdate><enddate>198603</enddate><creator>Anderson, R.</creator><creator>Joone, G.</creator><creator>van Rensburg, C. 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J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c329t-259874fe79dacec4913d5ea47eb9eb9a9d03bed8b557806fed7534e50289ce1f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Amoxicillin - pharmacology</topic><topic>Antibacterial agents</topic><topic>Antibiotics</topic><topic>Antibiotics. Antiinfectious agents. Antiparasitic agents</topic><topic>Antimicrobials</topic><topic>Bacteria</topic><topic>Biological and medical sciences</topic><topic>Chemiluminescence</topic><topic>Chronic granulomatous disease</topic><topic>Clindamycin - pharmacology</topic><topic>Erythromycin - pharmacology</topic><topic>Fluorescence</topic><topic>Fluorometry</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Methods</topic><topic>Microbial Sensitivity Tests</topic><topic>Neutrophils - drug effects</topic><topic>Neutrophils - microbiology</topic><topic>Original Articles</topic><topic>Pathogens</topic><topic>Phagocytes</topic><topic>Phagocytosis</topic><topic>Pharmacology. Drug treatments</topic><topic>Sodium Fluoride - pharmacology</topic><topic>Staphylococcus aureus - drug effects</topic><topic>Viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Anderson, R.</creatorcontrib><creatorcontrib>Joone, G.</creatorcontrib><creatorcontrib>van Rensburg, C. 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E. J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An in Vitro Investigation of the Intracellular Bioactivity of Amoxicillin, Clindamycin, and Erythromycin for Staphylococcus aureus</atitle><jtitle>The Journal of infectious diseases</jtitle><addtitle>J Infect Dis</addtitle><date>1986-03</date><risdate>1986</risdate><volume>153</volume><issue>3</issue><spage>593</spage><epage>600</epage><pages>593-600</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>The intraphagocytic bioactivities for Staphylococcus aureus of amoxicillin, clindamycin, and erythromycin (0.0075-20 µ/ml) were measured in human polymorphonuclear leukocytes (PMNLs) with the combination of a fluorochrome microassay and a radioassay. PMNLs with normal or depleted membrane-associated oxidative metabolism were used to investigate the interactions that may occur between the intrinsic O2 dependent antimicrobial systems of human phagocytes and antimicrobial agents in the elimination of intracellular microbial pathogens. Neutralization of 02-dependent antimicrobial systems with retention of phagocytic capacity was achieved with use of PMNLs.from four children with chronic granulomatous disease (COD) or NaF-pulsed normalPMNLs. None of the test antibiotics possessed intracellular bactericidal activity. Clindamycin and erythromycin possessed significant intracellular bacteriostatic activity relativ~ to the modest activity of amoxicillin. Optimal intracellular bioactivity of all three antibiotics was obtained with normal PMNLs relative to NaF-pulsed or COD PMNLs, a result indicating the existence of beneficial interactions between the antimicrobial agents and the O2 dependent antimicrobial systems of PMNLs.</abstract><cop>Chicago, IL</cop><pub>Oxford University Press</pub><pmid>3950443</pmid><doi>10.1093/infdis/153.3.593</doi><tpages>8</tpages></addata></record> |
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| ispartof | The Journal of infectious diseases, 1986-03, Vol.153 (3), p.593-600 |
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| language | eng |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing |
| subjects | Amoxicillin - pharmacology Antibacterial agents Antibiotics Antibiotics. Antiinfectious agents. Antiparasitic agents Antimicrobials Bacteria Biological and medical sciences Chemiluminescence Chronic granulomatous disease Clindamycin - pharmacology Erythromycin - pharmacology Fluorescence Fluorometry Humans Medical sciences Methods Microbial Sensitivity Tests Neutrophils - drug effects Neutrophils - microbiology Original Articles Pathogens Phagocytes Phagocytosis Pharmacology. Drug treatments Sodium Fluoride - pharmacology Staphylococcus aureus - drug effects Viability |
| title | An in Vitro Investigation of the Intracellular Bioactivity of Amoxicillin, Clindamycin, and Erythromycin for Staphylococcus aureus |
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