Determination of Kinetic Rate Constants for the Binding of Inhibitors to HIV-1 Protease and for the Association and Dissociation of Active Homodimer

Association and dissociation rate constants for a competitive inhibitor of HIV-1 protease were determined by a novel method employing a pair of integrated rate equations. This method, termed the paired progress curve method, is both rapid and reproducible. Progress curves, taken at a single concentr...

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Veröffentlicht in:	Biochemistry (Easton) 1994-10, Vol.33 (41), p.12527-12534
Hauptverfasser:	Pargellis, Christopher A, Morelock, Maurice M, Graham, Edward T, Kinkade, Peter, Pav, Susan, Lubbe, Klaus, Lamarre, Daniel, Anderson, Paul C
Format:	Artikel
Sprache:	eng
Schlagworte:	AIDS/HIV Binding, Competitive HIV Protease - chemistry HIV Protease - metabolism HIV Protease Inhibitors - metabolism HIV-1 - enzymology human immunodeficiency virus Kinetics Macromolecular Substances Regression Analysis
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