Peroxidase Labeling of IgMs Fragment of ABO Blood Group Specific Mouse Monoclonal IgM
A method for peroxidase labeling of the monomeric subunit (IgMs) of ABO blood group specific mouse monoclonal IgM is described. IgM was purified from a commercial monoclonal anti-B blood grouping reagent by a combination of salt precipitation, euglobulin precipitation, and gel filtration. IgM was mi...
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| Veröffentlicht in: | Bioconjugate chemistry 1994-05, Vol.5 (3), p.273-277 |
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| container_end_page | 277 |
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| container_issue | 3 |
| container_start_page | 273 |
| container_title | Bioconjugate chemistry |
| container_volume | 5 |
| creator | Yukawa, Nobuhiro Matsuda, Hirokazu Seo, Yasuhisa Suetomi, Katsutoshi Takahama, Keiichi |
| description | A method for peroxidase labeling of the monomeric subunit (IgMs) of ABO blood group specific mouse monoclonal IgM is described. IgM was purified from a commercial monoclonal anti-B blood grouping reagent by a combination of salt precipitation, euglobulin precipitation, and gel filtration. IgM was mildly reduced with L-cysteine to yield SH-bearing IgMs. Finally, IgMs was conjugated to horseradish peroxidase, into which SH-reacting maleimide groups had been introduced using N-succinimidyl 6-maleimidohexanoate, through the selective reaction between SH of IgMs and maleimide groups of peroxidase. |
| doi_str_mv | 10.1021/bc00027a014 |
| format | Article |
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IgM was purified from a commercial monoclonal anti-B blood grouping reagent by a combination of salt precipitation, euglobulin precipitation, and gel filtration. 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IgM was purified from a commercial monoclonal anti-B blood grouping reagent by a combination of salt precipitation, euglobulin precipitation, and gel filtration. 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| fulltext | fulltext |
| identifier | ISSN: 1043-1802 |
| ispartof | Bioconjugate chemistry, 1994-05, Vol.5 (3), p.273-277 |
| issn | 1043-1802 1520-4812 |
| language | eng |
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| source | MEDLINE; ACS Publications |
| subjects | ABO Blood-Group System - immunology Animals Antibodies, Monoclonal - chemistry Drug Stability Horseradish Peroxidase - chemistry Humans Immunoblotting Immunoconjugates - chemistry Immunoconjugates - isolation & purification Immunoenzyme Techniques Immunoglobulin M - chemistry In Vitro Techniques Maleimides - chemistry Mice Protein Conformation |
| title | Peroxidase Labeling of IgMs Fragment of ABO Blood Group Specific Mouse Monoclonal IgM |
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