Detection of functional oxytocin receptors on endometrium of pigs
Oxytocin (OT) stimulates phosphoinositide (PI) hydrolysis and prostaglandin (PG) F(2 alpha) secretion from the endometrium of cyclic pigs, but the presence of specific endometrial receptors for OT has not been demonstrated in this species. Two experiments were performed to detect the presence of fun...
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| Veröffentlicht in: | Biology of reproduction 1994-07, Vol.51 (1), p.92-98 |
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| creator | Whiteaker, S.S Mirando, M.A Becker, W.C Hostetler, C.E |
| description | Oxytocin (OT) stimulates phosphoinositide (PI) hydrolysis and prostaglandin (PG) F(2 alpha) secretion from the endometrium of cyclic pigs, but the presence of specific endometrial receptors for OT has not been demonstrated in this species. Two experiments were performed to detect the presence of functional OT receptors on endometrium collected 15 days post estrus from cyclic gilts. OT receptor density and K(d) were determined by receptor assay and Scatchard analysis. Hydrolysis of PI (i.e., incorporation [(3)H] inositol into total inositol phosphates) and PGF(2 alpha) secretion were studied with use of incubations of endometrial explants. Concentrations of PGF(2 alpha) were log-transformed for analysis of variance and are expressed as means +/- standard error of log-transformed data. In experiment 1, mean density and mean K(d) of OT receptors on endometrium of gilts were 29.2 +/- 5.54 fmol/mg protein and 1.59 +/- 0.23 nM, respectively. OT receptor density was significantly correlated with the ability of 100 nM OT to stimulate PI hydrolysis (r = 0.83, p 0.05) and PGF(2 alpha) secretion (r |
| doi_str_mv | 10.1095/biolreprod51.1.92 |
| format | Article |
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Two experiments were performed to detect the presence of functional OT receptors on endometrium collected 15 days post estrus from cyclic gilts. OT receptor density and K(d) were determined by receptor assay and Scatchard analysis. Hydrolysis of PI (i.e., incorporation [(3)H] inositol into total inositol phosphates) and PGF(2 alpha) secretion were studied with use of incubations of endometrial explants. Concentrations of PGF(2 alpha) were log-transformed for analysis of variance and are expressed as means +/- standard error of log-transformed data. In experiment 1, mean density and mean K(d) of OT receptors on endometrium of gilts were 29.2 +/- 5.54 fmol/mg protein and 1.59 +/- 0.23 nM, respectively. OT receptor density was significantly correlated with the ability of 100 nM OT to stimulate PI hydrolysis (r = 0.83, p 0.05) and PGF(2 alpha) secretion (r</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod51.1.92</identifier><identifier>PMID: 7918879</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Animals ; Biological and medical sciences ; CERDAS ; CHIMIORECEPTEUR ; Dinoprost - metabolism ; Endometrium - chemistry ; Estrus - physiology ; Female ; Fundamental and applied biological sciences. Psychology ; Hormone metabolism and regulation ; Hydrolysis ; Lypressin - pharmacology ; Mammalian female genital system ; OCYTOCINE ; OXITOCINA ; Oxytocin - metabolism ; Oxytocin - pharmacology ; Phosphatidylinositols - metabolism ; QUIMIORECEPTORS ; Receptors, Oxytocin - analysis ; Receptors, Oxytocin - metabolism ; Swine ; Tritium ; TRUIE ; UTERO ; UTERUS ; Vertebrates: reproduction</subject><ispartof>Biology of reproduction, 1994-07, Vol.51 (1), p.92-98</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4206156$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7918879$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Whiteaker, S.S</creatorcontrib><creatorcontrib>Mirando, M.A</creatorcontrib><creatorcontrib>Becker, W.C</creatorcontrib><creatorcontrib>Hostetler, C.E</creatorcontrib><title>Detection of functional oxytocin receptors on endometrium of pigs</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>Oxytocin (OT) stimulates phosphoinositide (PI) hydrolysis and prostaglandin (PG) F(2 alpha) secretion from the endometrium of cyclic pigs, but the presence of specific endometrial receptors for OT has not been demonstrated in this species. Two experiments were performed to detect the presence of functional OT receptors on endometrium collected 15 days post estrus from cyclic gilts. OT receptor density and K(d) were determined by receptor assay and Scatchard analysis. Hydrolysis of PI (i.e., incorporation [(3)H] inositol into total inositol phosphates) and PGF(2 alpha) secretion were studied with use of incubations of endometrial explants. Concentrations of PGF(2 alpha) were log-transformed for analysis of variance and are expressed as means +/- standard error of log-transformed data. In experiment 1, mean density and mean K(d) of OT receptors on endometrium of gilts were 29.2 +/- 5.54 fmol/mg protein and 1.59 +/- 0.23 nM, respectively. OT receptor density was significantly correlated with the ability of 100 nM OT to stimulate PI hydrolysis (r = 0.83, p 0.05) and PGF(2 alpha) secretion (r</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>CERDAS</subject><subject>CHIMIORECEPTEUR</subject><subject>Dinoprost - metabolism</subject><subject>Endometrium - chemistry</subject><subject>Estrus - physiology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hormone metabolism and regulation</subject><subject>Hydrolysis</subject><subject>Lypressin - pharmacology</subject><subject>Mammalian female genital system</subject><subject>OCYTOCINE</subject><subject>OXITOCINA</subject><subject>Oxytocin - metabolism</subject><subject>Oxytocin - pharmacology</subject><subject>Phosphatidylinositols - metabolism</subject><subject>QUIMIORECEPTORS</subject><subject>Receptors, Oxytocin - analysis</subject><subject>Receptors, Oxytocin - metabolism</subject><subject>Swine</subject><subject>Tritium</subject><subject>TRUIE</subject><subject>UTERO</subject><subject>UTERUS</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFz01LJDEQBuAgio6z_oAVFvog3no2lY_u5CjjJwge3DmHTLp6zNLdGZNuRv_9Rh3WulTB-_BCEfIT6AKolr_XPnQRtzE0Ehaw0OyAzEAyXdasUodkRimtSs4rfkJOU_pLKQjO-DE5rjUoVesZubrGEd3ow1CEtmin4fO2XRHe3sfg_FBEdLgdQ0xFNjg0occx-qn_8Fu_ST_IUWu7hGf7PSer25s_y_vy8enuYXn1WLZM8bFsUQoqBHN1rWoOayGE5I46bClXbg1KWkQNwukqO0uFbhxVeRqwDQfgc3L51ZvffZ0wjab3yWHX2QHDlExd1UwzkBn-2sNp3WNjttH3Nr6b_c85v9jnNjnbtdEOzqf_TDBagay-2YvfvOx8RJN623W5lJvdbifBgNEss_Mv1tpg7CbmptWzlrmBKv4PPcZ70w</recordid><startdate>19940701</startdate><enddate>19940701</enddate><creator>Whiteaker, S.S</creator><creator>Mirando, M.A</creator><creator>Becker, W.C</creator><creator>Hostetler, C.E</creator><general>Society for the Study of Reproduction</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19940701</creationdate><title>Detection of functional oxytocin receptors on endometrium of pigs</title><author>Whiteaker, S.S ; Mirando, M.A ; Becker, W.C ; Hostetler, C.E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f283t-fe540442c778731b44453c0cef038cb185aee914c96404a049dc08888d1ad3113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>CERDAS</topic><topic>CHIMIORECEPTEUR</topic><topic>Dinoprost - metabolism</topic><topic>Endometrium - chemistry</topic><topic>Estrus - physiology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hormone metabolism and regulation</topic><topic>Hydrolysis</topic><topic>Lypressin - pharmacology</topic><topic>Mammalian female genital system</topic><topic>OCYTOCINE</topic><topic>OXITOCINA</topic><topic>Oxytocin - metabolism</topic><topic>Oxytocin - pharmacology</topic><topic>Phosphatidylinositols - metabolism</topic><topic>QUIMIORECEPTORS</topic><topic>Receptors, Oxytocin - analysis</topic><topic>Receptors, Oxytocin - metabolism</topic><topic>Swine</topic><topic>Tritium</topic><topic>TRUIE</topic><topic>UTERO</topic><topic>UTERUS</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Whiteaker, S.S</creatorcontrib><creatorcontrib>Mirando, M.A</creatorcontrib><creatorcontrib>Becker, W.C</creatorcontrib><creatorcontrib>Hostetler, C.E</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Whiteaker, S.S</au><au>Mirando, M.A</au><au>Becker, W.C</au><au>Hostetler, C.E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of functional oxytocin receptors on endometrium of pigs</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>1994-07-01</date><risdate>1994</risdate><volume>51</volume><issue>1</issue><spage>92</spage><epage>98</epage><pages>92-98</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>Oxytocin (OT) stimulates phosphoinositide (PI) hydrolysis and prostaglandin (PG) F(2 alpha) secretion from the endometrium of cyclic pigs, but the presence of specific endometrial receptors for OT has not been demonstrated in this species. Two experiments were performed to detect the presence of functional OT receptors on endometrium collected 15 days post estrus from cyclic gilts. OT receptor density and K(d) were determined by receptor assay and Scatchard analysis. Hydrolysis of PI (i.e., incorporation [(3)H] inositol into total inositol phosphates) and PGF(2 alpha) secretion were studied with use of incubations of endometrial explants. Concentrations of PGF(2 alpha) were log-transformed for analysis of variance and are expressed as means +/- standard error of log-transformed data. In experiment 1, mean density and mean K(d) of OT receptors on endometrium of gilts were 29.2 +/- 5.54 fmol/mg protein and 1.59 +/- 0.23 nM, respectively. OT receptor density was significantly correlated with the ability of 100 nM OT to stimulate PI hydrolysis (r = 0.83, p 0.05) and PGF(2 alpha) secretion (r</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>7918879</pmid><doi>10.1095/biolreprod51.1.92</doi><tpages>7</tpages></addata></record> |
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| ispartof | Biology of reproduction, 1994-07, Vol.51 (1), p.92-98 |
| issn | 0006-3363 1529-7268 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Biological and medical sciences CERDAS CHIMIORECEPTEUR Dinoprost - metabolism Endometrium - chemistry Estrus - physiology Female Fundamental and applied biological sciences. Psychology Hormone metabolism and regulation Hydrolysis Lypressin - pharmacology Mammalian female genital system OCYTOCINE OXITOCINA Oxytocin - metabolism Oxytocin - pharmacology Phosphatidylinositols - metabolism QUIMIORECEPTORS Receptors, Oxytocin - analysis Receptors, Oxytocin - metabolism Swine Tritium TRUIE UTERO UTERUS Vertebrates: reproduction |
| title | Detection of functional oxytocin receptors on endometrium of pigs |
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