"Wired" Enzyme Electrodes for Amperometric Determination of Glucose or Lactate in the Presence of Interfering Substances
Glucose oxidase (GOX) or lactate oxidase (LOX) were immobilized in an osmium-based three-dimensional redox hydrogel that electrically connected the enzyme's redox centers to electrodes. The enzyme "wiring" hydrogel was formed by cross-linking poly(1-vinylimidazole) (PVI) complexed wit...
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| Veröffentlicht in: | Analytical chemistry (Washington) 1994-08, Vol.66 (15), p.2451-2457 |
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| creator | Ohara, Timothy J Rajagopalan, Ravi Heller, Adam |
| description | Glucose oxidase (GOX) or lactate oxidase (LOX) were immobilized in an osmium-based three-dimensional redox hydrogel that electrically connected the enzyme's redox centers to electrodes. The enzyme "wiring" hydrogel was formed by cross-linking poly(1-vinylimidazole) (PVI) complexed with Os-(4,4'-dimethylbpy)2Cl (termed PVI15-dmeOs) with poly(ethylene glycol) diglycidyl ether (peg). Glucose and lactate sensors exhibited typical limiting current densities of 250 and 500 microA/cm2, respectively. When the electrodes were poised at 200 mV (SCE), the currents resulting from electrooxidation of ascorbate, urate, acetaminophen, and L-cysteine were negligible. When a Nafion film was employed, the linear range was extended from 6 to 30 mM glucose and from 4 to 7 mM lactate. The redox potential of the gel-forming polymer was 95 mV (SCE). Glucose and lactate measurements performed in bovine calf serum correlated well with a substrate calibration in phosphate buffer. |
| doi_str_mv | 10.1021/ac00087a008 |
| format | Article |
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The enzyme "wiring" hydrogel was formed by cross-linking poly(1-vinylimidazole) (PVI) complexed with Os-(4,4'-dimethylbpy)2Cl (termed PVI15-dmeOs) with poly(ethylene glycol) diglycidyl ether (peg). Glucose and lactate sensors exhibited typical limiting current densities of 250 and 500 microA/cm2, respectively. When the electrodes were poised at 200 mV (SCE), the currents resulting from electrooxidation of ascorbate, urate, acetaminophen, and L-cysteine were negligible. When a Nafion film was employed, the linear range was extended from 6 to 30 mM glucose and from 4 to 7 mM lactate. The redox potential of the gel-forming polymer was 95 mV (SCE). Glucose and lactate measurements performed in bovine calf serum correlated well with a substrate calibration in phosphate buffer.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac00087a008</identifier><identifier>PMID: 8092486</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Animals ; Biochemistry ; Biological and medical sciences ; Biosensing Techniques ; Biosensors ; Biotechnology ; Blood ; Blood Glucose - analysis ; Calibration ; Cattle ; Enzymes ; Enzymes, Immobilized ; Fundamental and applied biological sciences. Psychology ; Glucose - analysis ; Glucose Oxidase - metabolism ; Lactates - analysis ; Lactates - blood ; Lactic Acid ; Methods. Procedures. Technologies ; Mixed Function Oxygenases - metabolism ; Oxidation-Reduction ; Sugar ; Various methods and equipments</subject><ispartof>Analytical chemistry (Washington), 1994-08, Vol.66 (15), p.2451-2457</ispartof><rights>1994 INIST-CNRS</rights><rights>Copyright American Chemical Society Aug 1, 1994</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a476t-2c589e25854fbcb05c01c5f89492108e0176a2ef64dcef4388e5efa53bfba7113</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ac00087a008$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ac00087a008$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2763,27075,27923,27924,56737,56787</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4210422$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8092486$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ohara, Timothy J</creatorcontrib><creatorcontrib>Rajagopalan, Ravi</creatorcontrib><creatorcontrib>Heller, Adam</creatorcontrib><title>"Wired" Enzyme Electrodes for Amperometric Determination of Glucose or Lactate in the Presence of Interfering Substances</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>Glucose oxidase (GOX) or lactate oxidase (LOX) were immobilized in an osmium-based three-dimensional redox hydrogel that electrically connected the enzyme's redox centers to electrodes. The enzyme "wiring" hydrogel was formed by cross-linking poly(1-vinylimidazole) (PVI) complexed with Os-(4,4'-dimethylbpy)2Cl (termed PVI15-dmeOs) with poly(ethylene glycol) diglycidyl ether (peg). Glucose and lactate sensors exhibited typical limiting current densities of 250 and 500 microA/cm2, respectively. When the electrodes were poised at 200 mV (SCE), the currents resulting from electrooxidation of ascorbate, urate, acetaminophen, and L-cysteine were negligible. When a Nafion film was employed, the linear range was extended from 6 to 30 mM glucose and from 4 to 7 mM lactate. The redox potential of the gel-forming polymer was 95 mV (SCE). Glucose and lactate measurements performed in bovine calf serum correlated well with a substrate calibration in phosphate buffer.</description><subject>Animals</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Biosensing Techniques</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Blood</subject><subject>Blood Glucose - analysis</subject><subject>Calibration</subject><subject>Cattle</subject><subject>Enzymes</subject><subject>Enzymes, Immobilized</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucose - analysis</subject><subject>Glucose Oxidase - metabolism</subject><subject>Lactates - analysis</subject><subject>Lactates - blood</subject><subject>Lactic Acid</subject><subject>Methods. Procedures. Technologies</subject><subject>Mixed Function Oxygenases - metabolism</subject><subject>Oxidation-Reduction</subject><subject>Sugar</subject><subject>Various methods and equipments</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0c1rFDEUAPAgSl2rJ89CWEQPMvqSmUyyx7Ld1sKihVaEXkIm-6KpM5M1yUDrX2_KLquIlwTyfnm8D0JeMnjPgLMPxgKAkqYcj8iMCQ5VqxR_TGblva64BHhKnqV0C8AYsPaIHClY8Ea1M3I3_-ojbuZ0Nf66H5CuerQ5hg0m6kKkJ8MWYxgwR2_pKWaMgx9N9mGkwdHzfrIhIS1wbWw2Gakfaf6O9DJiwtHig7oYyzeH0Y_f6NXUpWxKID0nT5zpE77Y38fky9nqevmxWn8-v1ierCvTyDZX3Aq1QC6UaFxnOxAWmBVOLZoFZ6AQmGwNR9c2G4uuqZVCgc6IunOdkYzVx-TNLu82hp8TpqwHnyz2vRkxTEnLVnIhGlXg_B94G6Y4lto0Z1IJENAW9G6HbAwpRXR6G_1g4r1moB-Wof9aRtGv9imnbsDNwe6nX-Kv93GTrOldLJPx6cCa0mHDeWHVjvmU8e4QNvGHbmUthb6-vNI3THy6OYOlPi3-7c4bm_408b8CfwO0X6yC</recordid><startdate>19940801</startdate><enddate>19940801</enddate><creator>Ohara, Timothy J</creator><creator>Rajagopalan, Ravi</creator><creator>Heller, Adam</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19940801</creationdate><title>"Wired" Enzyme Electrodes for Amperometric Determination of Glucose or Lactate in the Presence of Interfering Substances</title><author>Ohara, Timothy J ; Rajagopalan, Ravi ; Heller, Adam</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a476t-2c589e25854fbcb05c01c5f89492108e0176a2ef64dcef4388e5efa53bfba7113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>Biosensing Techniques</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Blood</topic><topic>Blood Glucose - analysis</topic><topic>Calibration</topic><topic>Cattle</topic><topic>Enzymes</topic><topic>Enzymes, Immobilized</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucose - analysis</topic><topic>Glucose Oxidase - metabolism</topic><topic>Lactates - analysis</topic><topic>Lactates - blood</topic><topic>Lactic Acid</topic><topic>Methods. Procedures. Technologies</topic><topic>Mixed Function Oxygenases - metabolism</topic><topic>Oxidation-Reduction</topic><topic>Sugar</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ohara, Timothy J</creatorcontrib><creatorcontrib>Rajagopalan, Ravi</creatorcontrib><creatorcontrib>Heller, Adam</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ohara, Timothy J</au><au>Rajagopalan, Ravi</au><au>Heller, Adam</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>"Wired" Enzyme Electrodes for Amperometric Determination of Glucose or Lactate in the Presence of Interfering Substances</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>1994-08-01</date><risdate>1994</risdate><volume>66</volume><issue>15</issue><spage>2451</spage><epage>2457</epage><pages>2451-2457</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>Glucose oxidase (GOX) or lactate oxidase (LOX) were immobilized in an osmium-based three-dimensional redox hydrogel that electrically connected the enzyme's redox centers to electrodes. The enzyme "wiring" hydrogel was formed by cross-linking poly(1-vinylimidazole) (PVI) complexed with Os-(4,4'-dimethylbpy)2Cl (termed PVI15-dmeOs) with poly(ethylene glycol) diglycidyl ether (peg). Glucose and lactate sensors exhibited typical limiting current densities of 250 and 500 microA/cm2, respectively. When the electrodes were poised at 200 mV (SCE), the currents resulting from electrooxidation of ascorbate, urate, acetaminophen, and L-cysteine were negligible. When a Nafion film was employed, the linear range was extended from 6 to 30 mM glucose and from 4 to 7 mM lactate. The redox potential of the gel-forming polymer was 95 mV (SCE). Glucose and lactate measurements performed in bovine calf serum correlated well with a substrate calibration in phosphate buffer.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>8092486</pmid><doi>10.1021/ac00087a008</doi><tpages>7</tpages></addata></record> |
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| ispartof | Analytical chemistry (Washington), 1994-08, Vol.66 (15), p.2451-2457 |
| issn | 0003-2700 1520-6882 |
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| source | American Chemical Society; MEDLINE |
| subjects | Animals Biochemistry Biological and medical sciences Biosensing Techniques Biosensors Biotechnology Blood Blood Glucose - analysis Calibration Cattle Enzymes Enzymes, Immobilized Fundamental and applied biological sciences. Psychology Glucose - analysis Glucose Oxidase - metabolism Lactates - analysis Lactates - blood Lactic Acid Methods. Procedures. Technologies Mixed Function Oxygenases - metabolism Oxidation-Reduction Sugar Various methods and equipments |
| title | "Wired" Enzyme Electrodes for Amperometric Determination of Glucose or Lactate in the Presence of Interfering Substances |
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