BY55 Monoclonal Antibody Delineates within Human Cord Blood and Bone Marrow Lymphocytes Distinct Cell Subsets Mediating Cytotoxic Activity
We had previously reported, using BY55 monoclonal antibody, a cell surface 80-kDa protein restricted to human functional peripheral blood cytotoxic lymphocytes with either natural killer CD3-or cytotoxic T lymphocyte CD3+CD8+phenotype. In the present report, we studied the cytotoxic lymphocytes in a...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1994-09, Vol.91 (19), p.9136-9140 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 91 |
| creator | Bensussan, A. Gluckman, E. El Marsafy, S. Schiavon, V. I.-G. Mansur Dausset, J. Boumsell, L. Carosella, E. |
| description | We had previously reported, using BY55 monoclonal antibody, a cell surface 80-kDa protein restricted to human functional peripheral blood cytotoxic lymphocytes with either natural killer CD3-or cytotoxic T lymphocyte CD3+CD8+phenotype. In the present report, we studied the cytotoxic lymphocytes in adult bone marrow and newborn cord blood as these organs are commonly used as sources of hematological stem cells for allogeneic transplantation. Our results showed that BY55 mAb labeled only 5-10% of the bone marrow lymphocytes, which included a major proportion of CD3+CD8+cytotoxic T lymphocytes. Interestingly, within cord blood cells, BY55+lymphocytes represented 20-35% of the lymphocytes corresponding exclusively to a CD3-cell subset. Furthermore, we detected in cord blood no cytotoxic T lymphocyte activity but we demonstrated that the CD3-BY55+cell subset contained the whole natural killer activity. |
| doi_str_mv | 10.1073/pnas.91.19.9136 |
| format | Article |
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Mansur ; Dausset, J. ; Boumsell, L. ; Carosella, E.</creator><creatorcontrib>Bensussan, A. ; Gluckman, E. ; El Marsafy, S. ; Schiavon, V. ; I.-G. Mansur ; Dausset, J. ; Boumsell, L. ; Carosella, E.</creatorcontrib><description>We had previously reported, using BY55 monoclonal antibody, a cell surface 80-kDa protein restricted to human functional peripheral blood cytotoxic lymphocytes with either natural killer CD3-or cytotoxic T lymphocyte CD3+CD8+phenotype. In the present report, we studied the cytotoxic lymphocytes in adult bone marrow and newborn cord blood as these organs are commonly used as sources of hematological stem cells for allogeneic transplantation. Our results showed that BY55 mAb labeled only 5-10% of the bone marrow lymphocytes, which included a major proportion of CD3+CD8+cytotoxic T lymphocytes. Interestingly, within cord blood cells, BY55+lymphocytes represented 20-35% of the lymphocytes corresponding exclusively to a CD3-cell subset. Furthermore, we detected in cord blood no cytotoxic T lymphocyte activity but we demonstrated that the CD3-BY55+cell subset contained the whole natural killer activity.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.91.19.9136</identifier><identifier>PMID: 8090781</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Antibodies, Monoclonal - immunology ; B lymphocytes ; Blood ; Bone marrow ; Bone Marrow Cells ; Cell lines ; Cellular biology ; Cord blood ; Cytotoxicity, Immunologic ; Fetal Blood - cytology ; Flow Cytometry ; Humans ; Immunity, Cellular ; Killer Cells, Natural - immunology ; Leukocytes ; Lymphocyte Subsets - immunology ; Lymphocytes ; Molecules ; Stem cells ; T lymphocytes ; T-Lymphocytes, Cytotoxic - immunology</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1994-09, Vol.91 (19), p.9136-9140</ispartof><rights>Copyright 1994 The National Academy of Sciences of the United States of America</rights><rights>Copyright National Academy of Sciences Sep 13, 1994</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c520t-de3e37cca7e43a0d50880c9a68eab44e90f04950faadc52c46e8a3b175b381c13</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/91/19.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2365985$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2365985$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8090781$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bensussan, A.</creatorcontrib><creatorcontrib>Gluckman, E.</creatorcontrib><creatorcontrib>El Marsafy, S.</creatorcontrib><creatorcontrib>Schiavon, V.</creatorcontrib><creatorcontrib>I.-G. Mansur</creatorcontrib><creatorcontrib>Dausset, J.</creatorcontrib><creatorcontrib>Boumsell, L.</creatorcontrib><creatorcontrib>Carosella, E.</creatorcontrib><title>BY55 Monoclonal Antibody Delineates within Human Cord Blood and Bone Marrow Lymphocytes Distinct Cell Subsets Mediating Cytotoxic Activity</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>We had previously reported, using BY55 monoclonal antibody, a cell surface 80-kDa protein restricted to human functional peripheral blood cytotoxic lymphocytes with either natural killer CD3-or cytotoxic T lymphocyte CD3+CD8+phenotype. In the present report, we studied the cytotoxic lymphocytes in adult bone marrow and newborn cord blood as these organs are commonly used as sources of hematological stem cells for allogeneic transplantation. Our results showed that BY55 mAb labeled only 5-10% of the bone marrow lymphocytes, which included a major proportion of CD3+CD8+cytotoxic T lymphocytes. Interestingly, within cord blood cells, BY55+lymphocytes represented 20-35% of the lymphocytes corresponding exclusively to a CD3-cell subset. Furthermore, we detected in cord blood no cytotoxic T lymphocyte activity but we demonstrated that the CD3-BY55+cell subset contained the whole natural killer activity.</description><subject>Antibodies, Monoclonal - immunology</subject><subject>B lymphocytes</subject><subject>Blood</subject><subject>Bone marrow</subject><subject>Bone Marrow Cells</subject><subject>Cell lines</subject><subject>Cellular biology</subject><subject>Cord blood</subject><subject>Cytotoxicity, Immunologic</subject><subject>Fetal Blood - cytology</subject><subject>Flow Cytometry</subject><subject>Humans</subject><subject>Immunity, Cellular</subject><subject>Killer Cells, Natural - immunology</subject><subject>Leukocytes</subject><subject>Lymphocyte Subsets - immunology</subject><subject>Lymphocytes</subject><subject>Molecules</subject><subject>Stem cells</subject><subject>T lymphocytes</subject><subject>T-Lymphocytes, Cytotoxic - immunology</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk-PEyEYxidGs66rZy9qiAc9tfsywAwkXrpddU3aeFAPngjD0C3NFCowuztfwU8tk9b656AXIDy_54U371MUTzFMMdTkfOdUnAo8xSKvpLpXnGIQeFJRAfeLU4CynnBa0ofFoxg3ACAYh5PihIOAmuPT4vvFV8bQ0juvO-9Uh2Yu2ca3A7o0nXVGJRPRrU1r69BVv1UOzX1o0UXnfYuUyyfvDFqqEPwtWgzb3drrYfRc2pis0wnNTdehT30TTYpoaVqr8v01mg_JJ39nNZrpZG9sGh4XD1aqi-bJYT8rvrx7-3l-NVl8fP9hPltMNCshTVpDDKm1VrWhREHLgHPQQlXcqIZSI2AFVDBYKdVmh6aV4Yo0uGYN4Vhjcla82dfd9c3WtNq4FFQnd8FuVRikV1b-qTi7ltf-RlJaV2W2vzrYg__Wm5jk1kadm1TO-D7KuqpLwgj_L4grxgWhI_jyL3Dj-5CHEWUJuKwxIeOz53tIBx9jMKvjhzHIMQpyjIIUWGIhxyhkx_Pf-zzyh9ln_fVBH40_1V8F5KrvumTuUiZf_JPMwLM9sInJhyNRkooJzsgP98LTzw</recordid><startdate>19940913</startdate><enddate>19940913</enddate><creator>Bensussan, A.</creator><creator>Gluckman, E.</creator><creator>El Marsafy, S.</creator><creator>Schiavon, V.</creator><creator>I.-G. Mansur</creator><creator>Dausset, J.</creator><creator>Boumsell, L.</creator><creator>Carosella, E.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><general>National Academy of Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19940913</creationdate><title>BY55 Monoclonal Antibody Delineates within Human Cord Blood and Bone Marrow Lymphocytes Distinct Cell Subsets Mediating Cytotoxic Activity</title><author>Bensussan, A. ; Gluckman, E. ; El Marsafy, S. ; Schiavon, V. ; I.-G. Mansur ; Dausset, J. ; Boumsell, L. ; Carosella, E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c520t-de3e37cca7e43a0d50880c9a68eab44e90f04950faadc52c46e8a3b175b381c13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Antibodies, Monoclonal - immunology</topic><topic>B lymphocytes</topic><topic>Blood</topic><topic>Bone marrow</topic><topic>Bone Marrow Cells</topic><topic>Cell lines</topic><topic>Cellular biology</topic><topic>Cord blood</topic><topic>Cytotoxicity, Immunologic</topic><topic>Fetal Blood - cytology</topic><topic>Flow Cytometry</topic><topic>Humans</topic><topic>Immunity, Cellular</topic><topic>Killer Cells, Natural - immunology</topic><topic>Leukocytes</topic><topic>Lymphocyte Subsets - immunology</topic><topic>Lymphocytes</topic><topic>Molecules</topic><topic>Stem cells</topic><topic>T lymphocytes</topic><topic>T-Lymphocytes, Cytotoxic - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bensussan, A.</creatorcontrib><creatorcontrib>Gluckman, E.</creatorcontrib><creatorcontrib>El Marsafy, S.</creatorcontrib><creatorcontrib>Schiavon, V.</creatorcontrib><creatorcontrib>I.-G. 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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1994-09, Vol.91 (19), p.9136-9140 |
| issn | 0027-8424 1091-6490 |
| language | eng |
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| source | Jstor Complete Legacy; MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Antibodies, Monoclonal - immunology B lymphocytes Blood Bone marrow Bone Marrow Cells Cell lines Cellular biology Cord blood Cytotoxicity, Immunologic Fetal Blood - cytology Flow Cytometry Humans Immunity, Cellular Killer Cells, Natural - immunology Leukocytes Lymphocyte Subsets - immunology Lymphocytes Molecules Stem cells T lymphocytes T-Lymphocytes, Cytotoxic - immunology |
| title | BY55 Monoclonal Antibody Delineates within Human Cord Blood and Bone Marrow Lymphocytes Distinct Cell Subsets Mediating Cytotoxic Activity |
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