Stoichiometry of Sulfolobus Ribosomal-Protein L12e in 50s Subunits Determined by Quantification of Immunoblots

A monoclonal antibody reactive with Sulfolobus solfataricus acidic ribosomal protein SsoL12e was prepared and employed to determine the stoichiometry of this protein in 50S ribosomal subunits by quantification of chloronaphthol-stained protein bands from immunoblots. Approximately four copies of Sso...

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Veröffentlicht in:	Biochemical and biophysical research communications 1994-09, Vol.203 (2), p.1140-1145
Hauptverfasser:	Casiano, C.A., Traut, R.R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies, Monoclonal Electrophoresis, Polyacrylamide Gel Escherichia coli - chemistry Escherichia coli Proteins Immunoblotting Ribosomal Proteins - analysis Ribosomal Proteins - chemistry Ribosomes - chemistry Sulfolobus - chemistry
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