Stoichiometry of Sulfolobus Ribosomal-Protein L12e in 50s Subunits Determined by Quantification of Immunoblots
A monoclonal antibody reactive with Sulfolobus solfataricus acidic ribosomal protein SsoL12e was prepared and employed to determine the stoichiometry of this protein in 50S ribosomal subunits by quantification of chloronaphthol-stained protein bands from immunoblots. Approximately four copies of Sso...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1994-09, Vol.203 (2), p.1140-1145 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Casiano, C.A. Traut, R.R. |
| description | A monoclonal antibody reactive with Sulfolobus solfataricus acidic ribosomal protein SsoL12e was prepared and employed to determine the stoichiometry of this protein in 50S ribosomal subunits by quantification of chloronaphthol-stained protein bands from immunoblots. Approximately four copies of SsoL12e were detected per 50S ribosome. This finding extends previous studies demonstrating the involvement of this protein in a multimeric protein complex in the ribosomal factor binding domain of Sulfolobus and strengthens the concept that this structural motif is a highly conserved and presumably critical feature of the ribosome. |
| doi_str_mv | 10.1006/bbrc.1994.2301 |
| format | Article |
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Approximately four copies of SsoL12e were detected per 50S ribosome. 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This finding extends previous studies demonstrating the involvement of this protein in a multimeric protein complex in the ribosomal factor binding domain of Sulfolobus and strengthens the concept that this structural motif is a highly conserved and presumably critical feature of the ribosome.</description><subject>Antibodies, Monoclonal</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Escherichia coli - chemistry</subject><subject>Escherichia coli Proteins</subject><subject>Immunoblotting</subject><subject>Ribosomal Proteins - analysis</subject><subject>Ribosomal Proteins - chemistry</subject><subject>Ribosomes - chemistry</subject><subject>Sulfolobus - chemistry</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1L7DAUhoN40XF0607oyl3Hk6RNp0vx415h4H54BXchSU8w0iaapML8e1tmcOfqXbzPeeE8hJxTWFEAcaV1NCvattWKcaAHZEGhhZJRqA7JAiaiZC19PiYnKb0CUFqJ9ogcraHlwNmC-MccnHlxYcAct0WwxePY29AHPabin9MhhUH15Z8YMjpfbCjDYsoa0gTq0bucilvMGAfnsSv0tvg7Kp-ddUZlF_y8-DAMow-6Dzmdkh9W9QnP9rkkT_d3_29-lZvfPx9urjel4bzNJROcM255JZiprVFQ1U1dg2VCGNNwXXcVXysqjBXIsKprJlpVIVil1hoaw5fkcrf7FsP7iCnLwSWDfa88hjHJRjSMMc4ncLUDTQwpRbTyLbpBxa2kIGfBchYsZ8FyFjwdXOyXRz1g94XvjU79etfj9N6HwyiTcegNdi6iybIL7rvpT22LiqE</recordid><startdate>19940915</startdate><enddate>19940915</enddate><creator>Casiano, C.A.</creator><creator>Traut, R.R.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19940915</creationdate><title>Stoichiometry of Sulfolobus Ribosomal-Protein L12e in 50s Subunits Determined by Quantification of Immunoblots</title><author>Casiano, C.A. ; Traut, R.R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c339t-263323f3462c5fca0457550f266cc73b5d438a16cf6e2e455269a4e0faa8b07c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Antibodies, Monoclonal</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Escherichia coli - chemistry</topic><topic>Escherichia coli Proteins</topic><topic>Immunoblotting</topic><topic>Ribosomal Proteins - analysis</topic><topic>Ribosomal Proteins - chemistry</topic><topic>Ribosomes - chemistry</topic><topic>Sulfolobus - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Casiano, C.A.</creatorcontrib><creatorcontrib>Traut, R.R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Casiano, C.A.</au><au>Traut, R.R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stoichiometry of Sulfolobus Ribosomal-Protein L12e in 50s Subunits Determined by Quantification of Immunoblots</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1994-09-15</date><risdate>1994</risdate><volume>203</volume><issue>2</issue><spage>1140</spage><epage>1145</epage><pages>1140-1145</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>A monoclonal antibody reactive with Sulfolobus solfataricus acidic ribosomal protein SsoL12e was prepared and employed to determine the stoichiometry of this protein in 50S ribosomal subunits by quantification of chloronaphthol-stained protein bands from immunoblots. Approximately four copies of SsoL12e were detected per 50S ribosome. This finding extends previous studies demonstrating the involvement of this protein in a multimeric protein complex in the ribosomal factor binding domain of Sulfolobus and strengthens the concept that this structural motif is a highly conserved and presumably critical feature of the ribosome.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>8093032</pmid><doi>10.1006/bbrc.1994.2301</doi><tpages>6</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1994-09, Vol.203 (2), p.1140-1145 |
| issn | 0006-291X 1090-2104 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Antibodies, Monoclonal Electrophoresis, Polyacrylamide Gel Escherichia coli - chemistry Escherichia coli Proteins Immunoblotting Ribosomal Proteins - analysis Ribosomal Proteins - chemistry Ribosomes - chemistry Sulfolobus - chemistry |
| title | Stoichiometry of Sulfolobus Ribosomal-Protein L12e in 50s Subunits Determined by Quantification of Immunoblots |
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