A 1.2-Mb YAC Contig Spans the quaking Region
We describe here a 1.2-Mb yeast artificial chromosome (YAC) contig within the region of mouse chromosome 17 between Brachyury (T) and D17Rp17e , and spanning the quaking (qk) region. We describe six new probes distributed across 1.2 Mb: D17Leh502, D17Leh503, D17Leh504, D17Leh505, D17Leh506, and D17L...
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| Veröffentlicht in: | Genomics (San Diego, Calif.) Calif.), 1994-05, Vol.21 (1), p.77-84 |
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| container_title | Genomics (San Diego, Calif.) |
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| creator | Cox, Roger D. Shedlovsky, Alexandra Hamvas, Renata Goldsworthy, Michelle Whittington, Joanne Connelly, Camille S. Dove, William F. Lehrach, Hans |
| description | We describe here a 1.2-Mb yeast artificial chromosome (YAC) contig within the region of mouse chromosome 17 between
Brachyury (T) and
D17Rp17e , and spanning the
quaking (qk) region. We describe six new probes distributed across 1.2 Mb:
D17Leh502, D17Leh503, D17Leh504, D17Leh505, D17Leh506, and
D17Leh507. Probes
D17Leh502 and
D17Leh507 are at the extreme ends of the YAC contig. With the exception of
D17Leh507, all of these probes are within a deletion associated with the
quaking
viable (qk
v)
allele of
quaking. We have positioned these probes on a detailed YAC physical map together with two previously published probes,
D17Leh508 and
D17Aus119. We show here that
D17Leh508 is also within the
qk
v
deletion. Genetic mapping of
D17Leh504 and
D17Leh507 on two high-resolution genetic crosses carrying
qk
v
and
quaking
lethal-1 (qk
l-1)
alleles shows that these probes do not recombine with
quaking and are therefore within 0.04 cM of
qk
v
and 0.05 cM of
qk
l-1
mutations. The deletion breakpoint contained within the YAC contig has been positioned to within 90 kb by restriction mapping of wildtype and mutant DNA. This contig will form the basis for identification and mapping of expressed sequences and for an investigation of genome organization. |
| doi_str_mv | 10.1006/geno.1994.1227 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_76719965</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0888754384712274</els_id><sourcerecordid>76719965</sourcerecordid><originalsourceid>FETCH-LOGICAL-c368t-4455e393e31c2016cba1fe5a13a37f15ebad0c3e194cdc50aadbaa81b800265e3</originalsourceid><addsrcrecordid>eNp1kDtPwzAURi0EKqWwsiFlQEwk-JE4zlhFvKQiJB4Dk-U4N8GQOq2dIPHvSdSoG9MdvvNd3XsQOic4IhjzmxpsG5EsiyNCaXqA5gSLLBQ85odojoUQYZrE7BideP-FMc6YoDM0E2NC8RxdLwMS0fCpCD6WeZC3tjN18LpR1gfdJwTbXn0bWwcvUJvWnqKjSjUezqa5QO93t2_5Q7h6vn_Ml6tQMy66MI6TBFjGgBFNMeG6UKSCRBGmWFqRBApVYs2AZLEudYKVKgulBCkExpQP1QW62u3duHbbg-_k2ngNTaMstL2XKU-Hj3kygNEO1K713kElN86slfuVBMtRjxz1yFGPHPUMhYtpc1-sodzjk48hv5xy5bVqKqesNn6PxSSllNABEzsMBgs_Bpz02oDVUBoHupNla_674A_XLX3V</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>76719965</pqid></control><display><type>article</type><title>A 1.2-Mb YAC Contig Spans the quaking Region</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Cox, Roger D. ; Shedlovsky, Alexandra ; Hamvas, Renata ; Goldsworthy, Michelle ; Whittington, Joanne ; Connelly, Camille S. ; Dove, William F. ; Lehrach, Hans</creator><creatorcontrib>Cox, Roger D. ; Shedlovsky, Alexandra ; Hamvas, Renata ; Goldsworthy, Michelle ; Whittington, Joanne ; Connelly, Camille S. ; Dove, William F. ; Lehrach, Hans</creatorcontrib><description>We describe here a 1.2-Mb yeast artificial chromosome (YAC) contig within the region of mouse chromosome 17 between
Brachyury (T) and
D17Rp17e , and spanning the
quaking (qk) region. We describe six new probes distributed across 1.2 Mb:
D17Leh502, D17Leh503, D17Leh504, D17Leh505, D17Leh506, and
D17Leh507. Probes
D17Leh502 and
D17Leh507 are at the extreme ends of the YAC contig. With the exception of
D17Leh507, all of these probes are within a deletion associated with the
quaking
viable (qk
v)
allele of
quaking. We have positioned these probes on a detailed YAC physical map together with two previously published probes,
D17Leh508 and
D17Aus119. We show here that
D17Leh508 is also within the
qk
v
deletion. Genetic mapping of
D17Leh504 and
D17Leh507 on two high-resolution genetic crosses carrying
qk
v
and
quaking
lethal-1 (qk
l-1)
alleles shows that these probes do not recombine with
quaking and are therefore within 0.04 cM of
qk
v
and 0.05 cM of
qk
l-1
mutations. The deletion breakpoint contained within the YAC contig has been positioned to within 90 kb by restriction mapping of wildtype and mutant DNA. This contig will form the basis for identification and mapping of expressed sequences and for an investigation of genome organization.</description><identifier>ISSN: 0888-7543</identifier><identifier>EISSN: 1089-8646</identifier><identifier>DOI: 10.1006/geno.1994.1227</identifier><identifier>PMID: 8088820</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Alleles ; Animals ; Biological and medical sciences ; Chromosome Walking ; Chromosomes, Artificial, Yeast ; Classical genetics, quantitative genetics, hybrids ; Crosses, Genetic ; DNA Probes ; Fundamental and applied biological sciences. Psychology ; Genetic Markers ; Genetics of eukaryotes. Biological and molecular evolution ; Mice ; Mice, Inbred DBA ; Mice, Quaking - genetics ; Phenotype ; Sequence Deletion ; Vertebrata</subject><ispartof>Genomics (San Diego, Calif.), 1994-05, Vol.21 (1), p.77-84</ispartof><rights>1994 Academic Press</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-4455e393e31c2016cba1fe5a13a37f15ebad0c3e194cdc50aadbaa81b800265e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0888754384712274$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4172212$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8088820$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cox, Roger D.</creatorcontrib><creatorcontrib>Shedlovsky, Alexandra</creatorcontrib><creatorcontrib>Hamvas, Renata</creatorcontrib><creatorcontrib>Goldsworthy, Michelle</creatorcontrib><creatorcontrib>Whittington, Joanne</creatorcontrib><creatorcontrib>Connelly, Camille S.</creatorcontrib><creatorcontrib>Dove, William F.</creatorcontrib><creatorcontrib>Lehrach, Hans</creatorcontrib><title>A 1.2-Mb YAC Contig Spans the quaking Region</title><title>Genomics (San Diego, Calif.)</title><addtitle>Genomics</addtitle><description>We describe here a 1.2-Mb yeast artificial chromosome (YAC) contig within the region of mouse chromosome 17 between
Brachyury (T) and
D17Rp17e , and spanning the
quaking (qk) region. We describe six new probes distributed across 1.2 Mb:
D17Leh502, D17Leh503, D17Leh504, D17Leh505, D17Leh506, and
D17Leh507. Probes
D17Leh502 and
D17Leh507 are at the extreme ends of the YAC contig. With the exception of
D17Leh507, all of these probes are within a deletion associated with the
quaking
viable (qk
v)
allele of
quaking. We have positioned these probes on a detailed YAC physical map together with two previously published probes,
D17Leh508 and
D17Aus119. We show here that
D17Leh508 is also within the
qk
v
deletion. Genetic mapping of
D17Leh504 and
D17Leh507 on two high-resolution genetic crosses carrying
qk
v
and
quaking
lethal-1 (qk
l-1)
alleles shows that these probes do not recombine with
quaking and are therefore within 0.04 cM of
qk
v
and 0.05 cM of
qk
l-1
mutations. The deletion breakpoint contained within the YAC contig has been positioned to within 90 kb by restriction mapping of wildtype and mutant DNA. This contig will form the basis for identification and mapping of expressed sequences and for an investigation of genome organization.</description><subject>Alleles</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Chromosome Walking</subject><subject>Chromosomes, Artificial, Yeast</subject><subject>Classical genetics, quantitative genetics, hybrids</subject><subject>Crosses, Genetic</subject><subject>DNA Probes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Markers</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Mice</subject><subject>Mice, Inbred DBA</subject><subject>Mice, Quaking - genetics</subject><subject>Phenotype</subject><subject>Sequence Deletion</subject><subject>Vertebrata</subject><issn>0888-7543</issn><issn>1089-8646</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kDtPwzAURi0EKqWwsiFlQEwk-JE4zlhFvKQiJB4Dk-U4N8GQOq2dIPHvSdSoG9MdvvNd3XsQOic4IhjzmxpsG5EsiyNCaXqA5gSLLBQ85odojoUQYZrE7BideP-FMc6YoDM0E2NC8RxdLwMS0fCpCD6WeZC3tjN18LpR1gfdJwTbXn0bWwcvUJvWnqKjSjUezqa5QO93t2_5Q7h6vn_Ml6tQMy66MI6TBFjGgBFNMeG6UKSCRBGmWFqRBApVYs2AZLEudYKVKgulBCkExpQP1QW62u3duHbbg-_k2ngNTaMstL2XKU-Hj3kygNEO1K713kElN86slfuVBMtRjxz1yFGPHPUMhYtpc1-sodzjk48hv5xy5bVqKqesNn6PxSSllNABEzsMBgs_Bpz02oDVUBoHupNla_674A_XLX3V</recordid><startdate>19940501</startdate><enddate>19940501</enddate><creator>Cox, Roger D.</creator><creator>Shedlovsky, Alexandra</creator><creator>Hamvas, Renata</creator><creator>Goldsworthy, Michelle</creator><creator>Whittington, Joanne</creator><creator>Connelly, Camille S.</creator><creator>Dove, William F.</creator><creator>Lehrach, Hans</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19940501</creationdate><title>A 1.2-Mb YAC Contig Spans the quaking Region</title><author>Cox, Roger D. ; Shedlovsky, Alexandra ; Hamvas, Renata ; Goldsworthy, Michelle ; Whittington, Joanne ; Connelly, Camille S. ; Dove, William F. ; Lehrach, Hans</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-4455e393e31c2016cba1fe5a13a37f15ebad0c3e194cdc50aadbaa81b800265e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Alleles</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Chromosome Walking</topic><topic>Chromosomes, Artificial, Yeast</topic><topic>Classical genetics, quantitative genetics, hybrids</topic><topic>Crosses, Genetic</topic><topic>DNA Probes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Markers</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Mice</topic><topic>Mice, Inbred DBA</topic><topic>Mice, Quaking - genetics</topic><topic>Phenotype</topic><topic>Sequence Deletion</topic><topic>Vertebrata</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cox, Roger D.</creatorcontrib><creatorcontrib>Shedlovsky, Alexandra</creatorcontrib><creatorcontrib>Hamvas, Renata</creatorcontrib><creatorcontrib>Goldsworthy, Michelle</creatorcontrib><creatorcontrib>Whittington, Joanne</creatorcontrib><creatorcontrib>Connelly, Camille S.</creatorcontrib><creatorcontrib>Dove, William F.</creatorcontrib><creatorcontrib>Lehrach, Hans</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Genomics (San Diego, Calif.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cox, Roger D.</au><au>Shedlovsky, Alexandra</au><au>Hamvas, Renata</au><au>Goldsworthy, Michelle</au><au>Whittington, Joanne</au><au>Connelly, Camille S.</au><au>Dove, William F.</au><au>Lehrach, Hans</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A 1.2-Mb YAC Contig Spans the quaking Region</atitle><jtitle>Genomics (San Diego, Calif.)</jtitle><addtitle>Genomics</addtitle><date>1994-05-01</date><risdate>1994</risdate><volume>21</volume><issue>1</issue><spage>77</spage><epage>84</epage><pages>77-84</pages><issn>0888-7543</issn><eissn>1089-8646</eissn><abstract>We describe here a 1.2-Mb yeast artificial chromosome (YAC) contig within the region of mouse chromosome 17 between
Brachyury (T) and
D17Rp17e , and spanning the
quaking (qk) region. We describe six new probes distributed across 1.2 Mb:
D17Leh502, D17Leh503, D17Leh504, D17Leh505, D17Leh506, and
D17Leh507. Probes
D17Leh502 and
D17Leh507 are at the extreme ends of the YAC contig. With the exception of
D17Leh507, all of these probes are within a deletion associated with the
quaking
viable (qk
v)
allele of
quaking. We have positioned these probes on a detailed YAC physical map together with two previously published probes,
D17Leh508 and
D17Aus119. We show here that
D17Leh508 is also within the
qk
v
deletion. Genetic mapping of
D17Leh504 and
D17Leh507 on two high-resolution genetic crosses carrying
qk
v
and
quaking
lethal-1 (qk
l-1)
alleles shows that these probes do not recombine with
quaking and are therefore within 0.04 cM of
qk
v
and 0.05 cM of
qk
l-1
mutations. The deletion breakpoint contained within the YAC contig has been positioned to within 90 kb by restriction mapping of wildtype and mutant DNA. This contig will form the basis for identification and mapping of expressed sequences and for an investigation of genome organization.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>8088820</pmid><doi>10.1006/geno.1994.1227</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0888-7543 |
| ispartof | Genomics (San Diego, Calif.), 1994-05, Vol.21 (1), p.77-84 |
| issn | 0888-7543 1089-8646 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76719965 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Alleles Animals Biological and medical sciences Chromosome Walking Chromosomes, Artificial, Yeast Classical genetics, quantitative genetics, hybrids Crosses, Genetic DNA Probes Fundamental and applied biological sciences. Psychology Genetic Markers Genetics of eukaryotes. Biological and molecular evolution Mice Mice, Inbred DBA Mice, Quaking - genetics Phenotype Sequence Deletion Vertebrata |
| title | A 1.2-Mb YAC Contig Spans the quaking Region |
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