Thyroid hormone deiodinases in purified primary glial cell cultures
Iodothyronine metabolism was studied in neuroglial cells prepared from neonatal rat cerebri. Astrocytes account for nearly all of the deiodinase activity in mixed glial cell cultures. The predominant pathway is 5-deiodination, which closely resembles the enzyme activity in homogenates of cerebral co...
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| Veröffentlicht in: | Brain research 1986-02, Vol.364 (2), p.382-385 |
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| container_title | Brain research |
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| creator | Cavalieri, Ralph R. Gavin, Laurence A. Cole, Ruth de Vellis, Jean |
| description | Iodothyronine metabolism was studied in neuroglial cells prepared from neonatal rat cerebri. Astrocytes account for nearly all of the deiodinase activity in mixed glial cell cultures. The predominant pathway is 5-deiodination, which closely resembles the enzyme activity in homogenates of cerebral cortex. Astrocytes cultured in serum-free chemically defined medium show a gradual decrease in 5-deiodination and an increase in 5′-deiodination. Exposure of cells to triiodothyronine partially reverses these changes. Thus, astrocytes contain enzymes for both major deiodinative pathways and may play a role in the regulation of thyroid hormone levels in the brain. |
| doi_str_mv | 10.1016/0006-8993(86)90852-8 |
| format | Article |
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Astrocytes account for nearly all of the deiodinase activity in mixed glial cell cultures. The predominant pathway is 5-deiodination, which closely resembles the enzyme activity in homogenates of cerebral cortex. Astrocytes cultured in serum-free chemically defined medium show a gradual decrease in 5-deiodination and an increase in 5′-deiodination. Exposure of cells to triiodothyronine partially reverses these changes. Thus, astrocytes contain enzymes for both major deiodinative pathways and may play a role in the regulation of thyroid hormone levels in the brain.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/0006-8993(86)90852-8</identifier><identifier>PMID: 3947976</identifier><identifier>CODEN: BRREAP</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Animals ; Animals, Newborn ; Applied sciences ; astrocyte ; Astrocytes - enzymology ; Biochemistry and metabolism ; Biological and medical sciences ; Cells, Cultured ; Central nervous system ; cerebrum ; deiodinase ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; glial cells ; Iodide Peroxidase - metabolism ; iodothyronine ; neuroglia ; Neuroglia - enzymology ; oligodendrocyte ; Oligodendroglia - enzymology ; Other techniques and industries ; Rats ; Telencephalon ; thyroid hormones ; thyroxine ; Thyroxine - metabolism ; triiodothyronine ; Triiodothyronine - metabolism ; Triiodothyronine - pharmacology ; Vertebrates: nervous system and sense organs</subject><ispartof>Brain research, 1986-02, Vol.364 (2), p.382-385</ispartof><rights>1986 Elsevier Science Publishers B.V. (Biomedical Division)</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-fb48cf00263898534c5c3f06a180aeee219cadab422c834bccf94a35dbb78cd03</citedby><cites>FETCH-LOGICAL-c475t-fb48cf00263898534c5c3f06a180aeee219cadab422c834bccf94a35dbb78cd03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0006-8993(86)90852-8$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8005699$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8029420$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3947976$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cavalieri, Ralph R.</creatorcontrib><creatorcontrib>Gavin, Laurence A.</creatorcontrib><creatorcontrib>Cole, Ruth</creatorcontrib><creatorcontrib>de Vellis, Jean</creatorcontrib><title>Thyroid hormone deiodinases in purified primary glial cell cultures</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>Iodothyronine metabolism was studied in neuroglial cells prepared from neonatal rat cerebri. Astrocytes account for nearly all of the deiodinase activity in mixed glial cell cultures. The predominant pathway is 5-deiodination, which closely resembles the enzyme activity in homogenates of cerebral cortex. Astrocytes cultured in serum-free chemically defined medium show a gradual decrease in 5-deiodination and an increase in 5′-deiodination. Exposure of cells to triiodothyronine partially reverses these changes. Thus, astrocytes contain enzymes for both major deiodinative pathways and may play a role in the regulation of thyroid hormone levels in the brain.</description><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Applied sciences</subject><subject>astrocyte</subject><subject>Astrocytes - enzymology</subject><subject>Biochemistry and metabolism</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Central nervous system</subject><subject>cerebrum</subject><subject>deiodinase</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>glial cells</subject><subject>Iodide Peroxidase - metabolism</subject><subject>iodothyronine</subject><subject>neuroglia</subject><subject>Neuroglia - enzymology</subject><subject>oligodendrocyte</subject><subject>Oligodendroglia - enzymology</subject><subject>Other techniques and industries</subject><subject>Rats</subject><subject>Telencephalon</subject><subject>thyroid hormones</subject><subject>thyroxine</subject><subject>Thyroxine - metabolism</subject><subject>triiodothyronine</subject><subject>Triiodothyronine - metabolism</subject><subject>Triiodothyronine - pharmacology</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLJDEQgIMo7jjrP9iFPojood3Ks5OLIIMvELzoOaSTas3S0z0m04L_3h6nmaNeUoT66vUR8ofCBQWq_gGAKrUx_EyrcwNaslLvkRnVFSsVE7BPZjvkFznK-f_45dzAITnkRlSmUjOyeHr9SH0MxWufln2HRcDYh9i5jLmIXbEaUmwihmKV4tKlj-Klja4tPLbjM7TrIWH-TQ4a12Y8nuKcPN9cPy3uyofH2_vF1UPpRSXXZVML7RsAprg2WnLhpecNKEc1OERk1HgXXC0Y85qL2vvGCMdlqOtK-wB8Tk63fVepfxswr-0y5s0mrsN-yLZSFVUU5I8gFUKClGoExRb0qc85YWOnMy0Fu5FsNwbtxqDVyn5Jtnos-zv1H-olhl3RZHXMn0x5l71rm-Q6H_MO08CMYPAzBlKNk-fkcovh6PY9YrLZR-w8hpjQr23o4_frfgKxt6US</recordid><startdate>19860205</startdate><enddate>19860205</enddate><creator>Cavalieri, Ralph R.</creator><creator>Gavin, Laurence A.</creator><creator>Cole, Ruth</creator><creator>de Vellis, Jean</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SQ</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19860205</creationdate><title>Thyroid hormone deiodinases in purified primary glial cell cultures</title><author>Cavalieri, Ralph R. ; Gavin, Laurence A. ; Cole, Ruth ; de Vellis, Jean</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-fb48cf00263898534c5c3f06a180aeee219cadab422c834bccf94a35dbb78cd03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Applied sciences</topic><topic>astrocyte</topic><topic>Astrocytes - enzymology</topic><topic>Biochemistry and metabolism</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Central nervous system</topic><topic>cerebrum</topic><topic>deiodinase</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>glial cells</topic><topic>Iodide Peroxidase - metabolism</topic><topic>iodothyronine</topic><topic>neuroglia</topic><topic>Neuroglia - enzymology</topic><topic>oligodendrocyte</topic><topic>Oligodendroglia - enzymology</topic><topic>Other techniques and industries</topic><topic>Rats</topic><topic>Telencephalon</topic><topic>thyroid hormones</topic><topic>thyroxine</topic><topic>Thyroxine - metabolism</topic><topic>triiodothyronine</topic><topic>Triiodothyronine - metabolism</topic><topic>Triiodothyronine - pharmacology</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cavalieri, Ralph R.</creatorcontrib><creatorcontrib>Gavin, Laurence A.</creatorcontrib><creatorcontrib>Cole, Ruth</creatorcontrib><creatorcontrib>de Vellis, Jean</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Endocrinology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cavalieri, Ralph R.</au><au>Gavin, Laurence A.</au><au>Cole, Ruth</au><au>de Vellis, Jean</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Thyroid hormone deiodinases in purified primary glial cell cultures</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>1986-02-05</date><risdate>1986</risdate><volume>364</volume><issue>2</issue><spage>382</spage><epage>385</epage><pages>382-385</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><coden>BRREAP</coden><abstract>Iodothyronine metabolism was studied in neuroglial cells prepared from neonatal rat cerebri. Astrocytes account for nearly all of the deiodinase activity in mixed glial cell cultures. The predominant pathway is 5-deiodination, which closely resembles the enzyme activity in homogenates of cerebral cortex. Astrocytes cultured in serum-free chemically defined medium show a gradual decrease in 5-deiodination and an increase in 5′-deiodination. Exposure of cells to triiodothyronine partially reverses these changes. Thus, astrocytes contain enzymes for both major deiodinative pathways and may play a role in the regulation of thyroid hormone levels in the brain.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>3947976</pmid><doi>10.1016/0006-8993(86)90852-8</doi><tpages>4</tpages></addata></record> |
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| ispartof | Brain research, 1986-02, Vol.364 (2), p.382-385 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Animals, Newborn Applied sciences astrocyte Astrocytes - enzymology Biochemistry and metabolism Biological and medical sciences Cells, Cultured Central nervous system cerebrum deiodinase Exact sciences and technology Fundamental and applied biological sciences. Psychology glial cells Iodide Peroxidase - metabolism iodothyronine neuroglia Neuroglia - enzymology oligodendrocyte Oligodendroglia - enzymology Other techniques and industries Rats Telencephalon thyroid hormones thyroxine Thyroxine - metabolism triiodothyronine Triiodothyronine - metabolism Triiodothyronine - pharmacology Vertebrates: nervous system and sense organs |
| title | Thyroid hormone deiodinases in purified primary glial cell cultures |
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