Cloning, sequencing and overexpression of the Desulfovibrio gigas ferredoxin gene in E. coli

We have cloned the gene encoding Desulfovibrio gigas ferredoxin using a photodigoxigenin-labelled probe synthesized with the polymerase chain reaction. The DNA sequence of the gene predicts a polypeptide of 58 residues after removal of the initial formyl methionine (polypeptide M r = 6,276). The fer...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	FEBS letters 1994-09, Vol.351 (3), p.401-404
Hauptverfasser:	Chen, Baowei, Menon, Nanda K., Dervertarnian, Lisa, Moura, Jose J.G., Przybyla, Alan E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Base Sequence Cloning, Molecular Desulfovibrio - genetics Desulfovibrio gigas DNA, Bacterial Escherichia coli - genetics Ferredoxin Ferredoxin gene Ferredoxins - genetics Iron-sulfur cluster Molecular Sequence Data Recombinant Proteins - genetics Spectrum Analysis
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!