Pathways of acetone's metabolism in the rat
Distributions of 14C were different from those of 13C in glucoses formed by livers of rats in diabetic ketosis and perfused with [2-14C]acetone and [2-13C]lactate. There was 32-73% of the 14C and 8-12% of the 13C in carbons 3 and 4 of the glucoses with the remaining 14C and 13C distributed about equ...
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| Veröffentlicht in: | The Journal of biological chemistry 1986-03, Vol.261 (9), p.3952-3957 |
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| creator | Kosugi, K Scofield, R F Chandramouli, V Kumaran, K Schumann, W C Landau, B R |
| description | Distributions of 14C were different from those of 13C in glucoses formed by livers of rats in diabetic ketosis and perfused with [2-14C]acetone and [2-13C]lactate. There was 32-73% of the 14C and 8-12% of the 13C in carbons 3 and 4 of the glucoses with the remaining 14C and 13C distributed about equally in the other carbons. Incorporations of 14C from [2-14C]acetone (14-39%) also exceeded those from [2-14C]pyruvate (8-10%) into carbons 3 and 4 of glucoses formed by hepatocytes from rats fed acetone or fasted. [2-14C]Acetone and [2-14C]pyruvate were infused into rats that were fed, fasted, given acetone in their drinking water, or in diabetic ketosis. Thirty-seven to 52% of the 14C in the glucoses formed was in their carbons 3 and 4 when the acetone was infused and 8 to 14% when the pyruvate was infused. [1,3-14C]Hydroxybutyrate was formed by the rats in diabetic ketosis given [2-14C]acetone. It is concluded that acetone is metabolized in rats to a large extent by a pathway in which lactate or its metabolic equivalent is not an intermediate and that pathway is via acetyl-CoA. via acetyl-CoA. |
| doi_str_mv | 10.1016/S0021-9258(17)35607-7 |
| format | Article |
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There was 32-73% of the 14C and 8-12% of the 13C in carbons 3 and 4 of the glucoses with the remaining 14C and 13C distributed about equally in the other carbons. Incorporations of 14C from [2-14C]acetone (14-39%) also exceeded those from [2-14C]pyruvate (8-10%) into carbons 3 and 4 of glucoses formed by hepatocytes from rats fed acetone or fasted. [2-14C]Acetone and [2-14C]pyruvate were infused into rats that were fed, fasted, given acetone in their drinking water, or in diabetic ketosis. Thirty-seven to 52% of the 14C in the glucoses formed was in their carbons 3 and 4 when the acetone was infused and 8 to 14% when the pyruvate was infused. [1,3-14C]Hydroxybutyrate was formed by the rats in diabetic ketosis given [2-14C]acetone. It is concluded that acetone is metabolized in rats to a large extent by a pathway in which lactate or its metabolic equivalent is not an intermediate and that pathway is via acetyl-CoA. via acetyl-CoA.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/S0021-9258(17)35607-7</identifier><identifier>PMID: 3081512</identifier><identifier>CODEN: JBCHA3</identifier><language>eng</language><publisher>Bethesda, MD: Elsevier Inc</publisher><subject>Acetone - metabolism ; Acetyl Coenzyme A - metabolism ; Animals ; Biological and medical sciences ; Diabetes Mellitus, Experimental - metabolism ; Diabetes. Impaired glucose tolerance ; Diabetic Ketoacidosis - metabolism ; Endocrine pancreas. Apud cells (diseases) ; Endocrinopathies ; Female ; Hydroxybutyrates - metabolism ; Lactates - metabolism ; Lactic Acid ; Liver - metabolism ; Medical sciences ; Perfusion ; Pyruvates - metabolism ; Pyruvic Acid ; Rats ; Rats, Inbred Strains</subject><ispartof>The Journal of biological chemistry, 1986-03, Vol.261 (9), p.3952-3957</ispartof><rights>1986 © 1986 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c463t-908e1f6d700e130aba101d766f9db4adf6a57bc85361df19420526295889f0f83</citedby><cites>FETCH-LOGICAL-c463t-908e1f6d700e130aba101d766f9db4adf6a57bc85361df19420526295889f0f83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7913197$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3081512$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kosugi, K</creatorcontrib><creatorcontrib>Scofield, R F</creatorcontrib><creatorcontrib>Chandramouli, V</creatorcontrib><creatorcontrib>Kumaran, K</creatorcontrib><creatorcontrib>Schumann, W C</creatorcontrib><creatorcontrib>Landau, B R</creatorcontrib><title>Pathways of acetone's metabolism in the rat</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Distributions of 14C were different from those of 13C in glucoses formed by livers of rats in diabetic ketosis and perfused with [2-14C]acetone and [2-13C]lactate. There was 32-73% of the 14C and 8-12% of the 13C in carbons 3 and 4 of the glucoses with the remaining 14C and 13C distributed about equally in the other carbons. Incorporations of 14C from [2-14C]acetone (14-39%) also exceeded those from [2-14C]pyruvate (8-10%) into carbons 3 and 4 of glucoses formed by hepatocytes from rats fed acetone or fasted. [2-14C]Acetone and [2-14C]pyruvate were infused into rats that were fed, fasted, given acetone in their drinking water, or in diabetic ketosis. Thirty-seven to 52% of the 14C in the glucoses formed was in their carbons 3 and 4 when the acetone was infused and 8 to 14% when the pyruvate was infused. [1,3-14C]Hydroxybutyrate was formed by the rats in diabetic ketosis given [2-14C]acetone. It is concluded that acetone is metabolized in rats to a large extent by a pathway in which lactate or its metabolic equivalent is not an intermediate and that pathway is via acetyl-CoA. via acetyl-CoA.</description><subject>Acetone - metabolism</subject><subject>Acetyl Coenzyme A - metabolism</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Diabetes Mellitus, Experimental - metabolism</subject><subject>Diabetes. Impaired glucose tolerance</subject><subject>Diabetic Ketoacidosis - metabolism</subject><subject>Endocrine pancreas. Apud cells (diseases)</subject><subject>Endocrinopathies</subject><subject>Female</subject><subject>Hydroxybutyrates - metabolism</subject><subject>Lactates - metabolism</subject><subject>Lactic Acid</subject><subject>Liver - metabolism</subject><subject>Medical sciences</subject><subject>Perfusion</subject><subject>Pyruvates - metabolism</subject><subject>Pyruvic Acid</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkF1LHDEUhoNU1nX1JwhDKW1FpuZMJsnkqhSpH7BgoRW8C5nMSSdlPtZkVvHfm3WXvW1ucnGe9z2Hh5AzoN-Agrj8TWkBuSp49RXkOeOCylwekDnQiuWMw-MHMt8jR-Q4xn80vVLBjMwYrYBDMScXv8zUvpjXmI0uMxanccAvMetxMvXY-dhnfsimFrNgphNy6EwX8XT3L8jD9c8_V7f58v7m7urHMrelYFOuaIXgRCMpRWDU1Cbd20ghnGrq0jROGC5rW3EmoHGgyoLyQhSKV5Vy1FVsQT5ve1dhfFpjnHTvo8WuMwOO66ilkMBAlgnkW9CGMcaATq-C70141UD1RpJ-l6Q3BjRI_S5Jy5Q72y1Y1z02-9TOSpp_2s1NtKZzwQzWxz0mVVqvNjUft1jr_7YvPqCu_Whb7HUh0lLNFN90fd9CmIw9eww6Wo-DxSYF7KSb0f_n2jeoi4wa</recordid><startdate>19860325</startdate><enddate>19860325</enddate><creator>Kosugi, K</creator><creator>Scofield, R F</creator><creator>Chandramouli, V</creator><creator>Kumaran, K</creator><creator>Schumann, W C</creator><creator>Landau, B R</creator><general>Elsevier Inc</general><general>American Society for Biochemistry and Molecular Biology</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19860325</creationdate><title>Pathways of acetone's metabolism in the rat</title><author>Kosugi, K ; Scofield, R F ; Chandramouli, V ; Kumaran, K ; Schumann, W C ; Landau, B R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c463t-908e1f6d700e130aba101d766f9db4adf6a57bc85361df19420526295889f0f83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Acetone - metabolism</topic><topic>Acetyl Coenzyme A - metabolism</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Diabetes Mellitus, Experimental - metabolism</topic><topic>Diabetes. Impaired glucose tolerance</topic><topic>Diabetic Ketoacidosis - metabolism</topic><topic>Endocrine pancreas. Apud cells (diseases)</topic><topic>Endocrinopathies</topic><topic>Female</topic><topic>Hydroxybutyrates - metabolism</topic><topic>Lactates - metabolism</topic><topic>Lactic Acid</topic><topic>Liver - metabolism</topic><topic>Medical sciences</topic><topic>Perfusion</topic><topic>Pyruvates - metabolism</topic><topic>Pyruvic Acid</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kosugi, K</creatorcontrib><creatorcontrib>Scofield, R F</creatorcontrib><creatorcontrib>Chandramouli, V</creatorcontrib><creatorcontrib>Kumaran, K</creatorcontrib><creatorcontrib>Schumann, W C</creatorcontrib><creatorcontrib>Landau, B R</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kosugi, K</au><au>Scofield, R F</au><au>Chandramouli, V</au><au>Kumaran, K</au><au>Schumann, W C</au><au>Landau, B R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pathways of acetone's metabolism in the rat</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1986-03-25</date><risdate>1986</risdate><volume>261</volume><issue>9</issue><spage>3952</spage><epage>3957</epage><pages>3952-3957</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><coden>JBCHA3</coden><abstract>Distributions of 14C were different from those of 13C in glucoses formed by livers of rats in diabetic ketosis and perfused with [2-14C]acetone and [2-13C]lactate. There was 32-73% of the 14C and 8-12% of the 13C in carbons 3 and 4 of the glucoses with the remaining 14C and 13C distributed about equally in the other carbons. Incorporations of 14C from [2-14C]acetone (14-39%) also exceeded those from [2-14C]pyruvate (8-10%) into carbons 3 and 4 of glucoses formed by hepatocytes from rats fed acetone or fasted. [2-14C]Acetone and [2-14C]pyruvate were infused into rats that were fed, fasted, given acetone in their drinking water, or in diabetic ketosis. Thirty-seven to 52% of the 14C in the glucoses formed was in their carbons 3 and 4 when the acetone was infused and 8 to 14% when the pyruvate was infused. [1,3-14C]Hydroxybutyrate was formed by the rats in diabetic ketosis given [2-14C]acetone. It is concluded that acetone is metabolized in rats to a large extent by a pathway in which lactate or its metabolic equivalent is not an intermediate and that pathway is via acetyl-CoA. via acetyl-CoA.</abstract><cop>Bethesda, MD</cop><pub>Elsevier Inc</pub><pmid>3081512</pmid><doi>10.1016/S0021-9258(17)35607-7</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acetone - metabolism Acetyl Coenzyme A - metabolism Animals Biological and medical sciences Diabetes Mellitus, Experimental - metabolism Diabetes. Impaired glucose tolerance Diabetic Ketoacidosis - metabolism Endocrine pancreas. Apud cells (diseases) Endocrinopathies Female Hydroxybutyrates - metabolism Lactates - metabolism Lactic Acid Liver - metabolism Medical sciences Perfusion Pyruvates - metabolism Pyruvic Acid Rats Rats, Inbred Strains |
| title | Pathways of acetone's metabolism in the rat |
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