Killing of Borrelia burgdorferi by macrophages is dependent on oxygen radicals and nitric oxide and can be enhanced by antibodies to outer surface proteins of the spirochete
Interaction of B. burgdorferi organisms with mouse bone marrow-derived macrophages (BMMΦ) leads to phagocytosis of microorganisms, induction of nitric oxide (NO) and superoxide radicals (O 2 − by BMMΦ and killing of spirochetes. Destruction of spirochetes by BMMΦ was quantified by a new method based...
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| Veröffentlicht in: | Immunology letters 1994-05, Vol.40 (2), p.139-146 |
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| creator | Modolell, M. Schaible, U.E. Rittig, M. Simon, M.M. |
| description | Interaction of
B. burgdorferi organisms with mouse bone marrow-derived macrophages (BMMΦ) leads to phagocytosis of microorganisms, induction of nitric oxide (NO) and superoxide radicals (O
2
− by BMMΦ and killing of spirochetes. Destruction of spirochetes by BMMΦ was quantified by a new method based on the release of radioactivity from spirochetes pre-labelled with [
3H]adenine. Uptake of
B. burgdorferi by BMMΦ, which mainly occurs by coiling phagocytosis, generation of NO and O
2
− radicals as well as killing of spirochetes were significantly enhanced by pre-opsonization of spirochetes with monoclonal antibodies (mAb) to the outer surface proteins A and B but not with those to the periplasmic flagellin. Addition of inhibitors specific for NO and O
2
− radical synthesis either separately or together to cultures of BMMΦ and spirochetes resulted in only partial reduction of the killing potential of effector cells. The data indicate that NO and O
2 radicals are necessary, but not sufficient, for complete elimination of
B. burgdorferi by macrophages. Together with previous findings that protection against
B. burgdorferi infection is conveyed by humoral immune responses the present data indicate that one of the important functions of specific antibodies is their participation in macrophage-mediated control of spirochetes. |
| doi_str_mv | 10.1016/0165-2478(94)90185-6 |
| format | Article |
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B. burgdorferi organisms with mouse bone marrow-derived macrophages (BMMΦ) leads to phagocytosis of microorganisms, induction of nitric oxide (NO) and superoxide radicals (O
2
− by BMMΦ and killing of spirochetes. Destruction of spirochetes by BMMΦ was quantified by a new method based on the release of radioactivity from spirochetes pre-labelled with [
3H]adenine. Uptake of
B. burgdorferi by BMMΦ, which mainly occurs by coiling phagocytosis, generation of NO and O
2
− radicals as well as killing of spirochetes were significantly enhanced by pre-opsonization of spirochetes with monoclonal antibodies (mAb) to the outer surface proteins A and B but not with those to the periplasmic flagellin. Addition of inhibitors specific for NO and O
2
− radical synthesis either separately or together to cultures of BMMΦ and spirochetes resulted in only partial reduction of the killing potential of effector cells. The data indicate that NO and O
2 radicals are necessary, but not sufficient, for complete elimination of
B. burgdorferi by macrophages. Together with previous findings that protection against
B. burgdorferi infection is conveyed by humoral immune responses the present data indicate that one of the important functions of specific antibodies is their participation in macrophage-mediated control of spirochetes.</description><identifier>ISSN: 0165-2478</identifier><identifier>EISSN: 1879-0542</identifier><identifier>DOI: 10.1016/0165-2478(94)90185-6</identifier><identifier>PMID: 8088871</identifier><identifier>CODEN: IMLED6</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Antibodies, Bacterial - immunology ; Antibodies, Monoclonal ; Antigens, Surface - immunology ; B. burgdorferi ; Bacterial diseases ; Bacterial Outer Membrane Proteins - immunology ; Bacterial Vaccines ; Biological and medical sciences ; Bone Marrow Cells ; Borrelia burgdorferi ; Borrelia burgdorferi Group - immunology ; Borrelia burgdorferi Group - ultrastructure ; Cells, Cultured ; Cytotoxicity, Immunologic - immunology ; Experimental bacterial diseases and models ; Female ; Infectious diseases ; Lipoproteins ; Lyme disease ; Lyme Disease - immunology ; Macrophage ; Macrophages - immunology ; Macrophages - ultrastructure ; Medical sciences ; Mice ; Mice, Inbred AKR ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Mice, SCID ; Nitric oxide ; Nitric Oxide - biosynthesis ; Nitric Oxide - physiology ; Phagocytosis ; Spirochete ; Superoxide ; Superoxides - metabolism</subject><ispartof>Immunology letters, 1994-05, Vol.40 (2), p.139-146</ispartof><rights>1994</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-65aa1e84af6d02f0381bffd23c91636fa962c5bbe1ed69b346bef42620486ef83</citedby><cites>FETCH-LOGICAL-c417t-65aa1e84af6d02f0381bffd23c91636fa962c5bbe1ed69b346bef42620486ef83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0165247894901856$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4096634$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8088871$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Modolell, M.</creatorcontrib><creatorcontrib>Schaible, U.E.</creatorcontrib><creatorcontrib>Rittig, M.</creatorcontrib><creatorcontrib>Simon, M.M.</creatorcontrib><title>Killing of Borrelia burgdorferi by macrophages is dependent on oxygen radicals and nitric oxide and can be enhanced by antibodies to outer surface proteins of the spirochete</title><title>Immunology letters</title><addtitle>Immunol Lett</addtitle><description>Interaction of
B. burgdorferi organisms with mouse bone marrow-derived macrophages (BMMΦ) leads to phagocytosis of microorganisms, induction of nitric oxide (NO) and superoxide radicals (O
2
− by BMMΦ and killing of spirochetes. Destruction of spirochetes by BMMΦ was quantified by a new method based on the release of radioactivity from spirochetes pre-labelled with [
3H]adenine. Uptake of
B. burgdorferi by BMMΦ, which mainly occurs by coiling phagocytosis, generation of NO and O
2
− radicals as well as killing of spirochetes were significantly enhanced by pre-opsonization of spirochetes with monoclonal antibodies (mAb) to the outer surface proteins A and B but not with those to the periplasmic flagellin. Addition of inhibitors specific for NO and O
2
− radical synthesis either separately or together to cultures of BMMΦ and spirochetes resulted in only partial reduction of the killing potential of effector cells. The data indicate that NO and O
2 radicals are necessary, but not sufficient, for complete elimination of
B. burgdorferi by macrophages. Together with previous findings that protection against
B. burgdorferi infection is conveyed by humoral immune responses the present data indicate that one of the important functions of specific antibodies is their participation in macrophage-mediated control of spirochetes.</description><subject>Animals</subject><subject>Antibodies, Bacterial - immunology</subject><subject>Antibodies, Monoclonal</subject><subject>Antigens, Surface - immunology</subject><subject>B. burgdorferi</subject><subject>Bacterial diseases</subject><subject>Bacterial Outer Membrane Proteins - immunology</subject><subject>Bacterial Vaccines</subject><subject>Biological and medical sciences</subject><subject>Bone Marrow Cells</subject><subject>Borrelia burgdorferi</subject><subject>Borrelia burgdorferi Group - immunology</subject><subject>Borrelia burgdorferi Group - ultrastructure</subject><subject>Cells, Cultured</subject><subject>Cytotoxicity, Immunologic - immunology</subject><subject>Experimental bacterial diseases and models</subject><subject>Female</subject><subject>Infectious diseases</subject><subject>Lipoproteins</subject><subject>Lyme disease</subject><subject>Lyme Disease - immunology</subject><subject>Macrophage</subject><subject>Macrophages - immunology</subject><subject>Macrophages - ultrastructure</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred AKR</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, SCID</subject><subject>Nitric oxide</subject><subject>Nitric Oxide - biosynthesis</subject><subject>Nitric Oxide - physiology</subject><subject>Phagocytosis</subject><subject>Spirochete</subject><subject>Superoxide</subject><subject>Superoxides - metabolism</subject><issn>0165-2478</issn><issn>1879-0542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2KFDEUhYMoY9v6BgpZiOiiNKlKpVKbAR38wwE3ug75uemOVCdlkhL7oXxHU9NNL3URAjnnnlzOh9BTSl5TQvmbevqmZYN4ObJXI6Gib_g9tKFiGBvSs_Y-2lwsD9GjnH8QQvuOdVfoShAhxEA36M8XP00-7HB0-F1MCSavsF7SzsbkIHmsj_igTIrzXu0gY5-xhRmChVBwDDj-Pu4g4KSsN2rKWAWLgy_Jmyp5C3cPRgWsAUPYq2DArpkqFK-j9TWyRByXAgnnJTllAM8pFvAhrzuVPeA8-xTNHgo8Rg9c_QWenO8t-v7h_bebT83t14-fb97eNobRoTS8V4qCYMpxS1pHOkG1c7btzEh5x50aeWt6rYGC5aPuGNfgWMtbwgQHJ7otenHKrav8XCAXefDZwDSpAHHJcuADbfue_tdIOR_Gka5GdjLWKnNO4OSc_EGlo6RErjjlykqurOTI5B1OyevYs3P-og9gL0NnflV_ftZVrv27VAv2-WJjZOS8Et-i65MNamm_PCSZjYeVhU9girTR_3uPv9_IvzI</recordid><startdate>19940501</startdate><enddate>19940501</enddate><creator>Modolell, M.</creator><creator>Schaible, U.E.</creator><creator>Rittig, M.</creator><creator>Simon, M.M.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19940501</creationdate><title>Killing of Borrelia burgdorferi by macrophages is dependent on oxygen radicals and nitric oxide and can be enhanced by antibodies to outer surface proteins of the spirochete</title><author>Modolell, M. ; Schaible, U.E. ; Rittig, M. ; Simon, M.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-65aa1e84af6d02f0381bffd23c91636fa962c5bbe1ed69b346bef42620486ef83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>Antibodies, Bacterial - immunology</topic><topic>Antibodies, Monoclonal</topic><topic>Antigens, Surface - immunology</topic><topic>B. burgdorferi</topic><topic>Bacterial diseases</topic><topic>Bacterial Outer Membrane Proteins - immunology</topic><topic>Bacterial Vaccines</topic><topic>Biological and medical sciences</topic><topic>Bone Marrow Cells</topic><topic>Borrelia burgdorferi</topic><topic>Borrelia burgdorferi Group - immunology</topic><topic>Borrelia burgdorferi Group - ultrastructure</topic><topic>Cells, Cultured</topic><topic>Cytotoxicity, Immunologic - immunology</topic><topic>Experimental bacterial diseases and models</topic><topic>Female</topic><topic>Infectious diseases</topic><topic>Lipoproteins</topic><topic>Lyme disease</topic><topic>Lyme Disease - immunology</topic><topic>Macrophage</topic><topic>Macrophages - immunology</topic><topic>Macrophages - ultrastructure</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred AKR</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, SCID</topic><topic>Nitric oxide</topic><topic>Nitric Oxide - biosynthesis</topic><topic>Nitric Oxide - physiology</topic><topic>Phagocytosis</topic><topic>Spirochete</topic><topic>Superoxide</topic><topic>Superoxides - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Modolell, M.</creatorcontrib><creatorcontrib>Schaible, U.E.</creatorcontrib><creatorcontrib>Rittig, M.</creatorcontrib><creatorcontrib>Simon, M.M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Immunology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Modolell, M.</au><au>Schaible, U.E.</au><au>Rittig, M.</au><au>Simon, M.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Killing of Borrelia burgdorferi by macrophages is dependent on oxygen radicals and nitric oxide and can be enhanced by antibodies to outer surface proteins of the spirochete</atitle><jtitle>Immunology letters</jtitle><addtitle>Immunol Lett</addtitle><date>1994-05-01</date><risdate>1994</risdate><volume>40</volume><issue>2</issue><spage>139</spage><epage>146</epage><pages>139-146</pages><issn>0165-2478</issn><eissn>1879-0542</eissn><coden>IMLED6</coden><abstract>Interaction of
B. burgdorferi organisms with mouse bone marrow-derived macrophages (BMMΦ) leads to phagocytosis of microorganisms, induction of nitric oxide (NO) and superoxide radicals (O
2
− by BMMΦ and killing of spirochetes. Destruction of spirochetes by BMMΦ was quantified by a new method based on the release of radioactivity from spirochetes pre-labelled with [
3H]adenine. Uptake of
B. burgdorferi by BMMΦ, which mainly occurs by coiling phagocytosis, generation of NO and O
2
− radicals as well as killing of spirochetes were significantly enhanced by pre-opsonization of spirochetes with monoclonal antibodies (mAb) to the outer surface proteins A and B but not with those to the periplasmic flagellin. Addition of inhibitors specific for NO and O
2
− radical synthesis either separately or together to cultures of BMMΦ and spirochetes resulted in only partial reduction of the killing potential of effector cells. The data indicate that NO and O
2 radicals are necessary, but not sufficient, for complete elimination of
B. burgdorferi by macrophages. Together with previous findings that protection against
B. burgdorferi infection is conveyed by humoral immune responses the present data indicate that one of the important functions of specific antibodies is their participation in macrophage-mediated control of spirochetes.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>8088871</pmid><doi>10.1016/0165-2478(94)90185-6</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0165-2478 |
| ispartof | Immunology letters, 1994-05, Vol.40 (2), p.139-146 |
| issn | 0165-2478 1879-0542 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76712551 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Antibodies, Bacterial - immunology Antibodies, Monoclonal Antigens, Surface - immunology B. burgdorferi Bacterial diseases Bacterial Outer Membrane Proteins - immunology Bacterial Vaccines Biological and medical sciences Bone Marrow Cells Borrelia burgdorferi Borrelia burgdorferi Group - immunology Borrelia burgdorferi Group - ultrastructure Cells, Cultured Cytotoxicity, Immunologic - immunology Experimental bacterial diseases and models Female Infectious diseases Lipoproteins Lyme disease Lyme Disease - immunology Macrophage Macrophages - immunology Macrophages - ultrastructure Medical sciences Mice Mice, Inbred AKR Mice, Inbred BALB C Mice, Inbred C57BL Mice, SCID Nitric oxide Nitric Oxide - biosynthesis Nitric Oxide - physiology Phagocytosis Spirochete Superoxide Superoxides - metabolism |
| title | Killing of Borrelia burgdorferi by macrophages is dependent on oxygen radicals and nitric oxide and can be enhanced by antibodies to outer surface proteins of the spirochete |
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