Numerical evaluation of changes in the cytoplasmic microtubule complex of C3H mouse cells by optical diffractometry and of changes in cell shape by fourier analysis
Mo mouse cells in culture on glass were treated with taxol, or nocodazole, or incubated at 4°C to alter their cytoplasmic microtubule complex (CMTC). From each treated group and from an untreated group, 30 cells stained with an antiserum against tubulin, were photographed under the photomicroscope,...
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| Veröffentlicht in: | Cytometry (New York, N.Y.) N.Y.), 1986-01, Vol.7 (1), p.18-24 |
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| creator | Mareel, Marc M. De Bruyne, Georges K. Ostrowski, Kazimierz Rozycka, Monika Strojny, Pawel Dziedzic‐Goclawska, Anna Lenczowski, Stanislaw Grzesik, Wojcech Kieler, Jorgen |
| description | Mo mouse cells in culture on glass were treated with taxol, or nocodazole, or incubated at 4°C to alter their cytoplasmic microtubule complex (CMTC). From each treated group and from an untreated group, 30 cells stained with an antiserum against tubulin, were photographed under the photomicroscope, and negatives were analysed by optical diffractometry. Differences between groups of cells were tested by variance analysis. Phasecontrast micrographs of the same cells were used for Fourier analysis of cell shape. Both types of analyses provided numerical objective data about changes in the CMTC and in cell shape that were typical for the kind of treatment. We conclude that optical diffratometry of immunostained cells and Fourier analysis of cell shape are complementary to photomicroscopy for the study of the CMTC in cell populations cultured on an artificial substrate. |
| doi_str_mv | 10.1002/cyto.990070104 |
| format | Article |
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From each treated group and from an untreated group, 30 cells stained with an antiserum against tubulin, were photographed under the photomicroscope, and negatives were analysed by optical diffractometry. Differences between groups of cells were tested by variance analysis. Phasecontrast micrographs of the same cells were used for Fourier analysis of cell shape. Both types of analyses provided numerical objective data about changes in the CMTC and in cell shape that were typical for the kind of treatment. We conclude that optical diffratometry of immunostained cells and Fourier analysis of cell shape are complementary to photomicroscopy for the study of the CMTC in cell populations cultured on an artificial substrate.</description><identifier>ISSN: 0196-4763</identifier><identifier>EISSN: 1097-0320</identifier><identifier>DOI: 10.1002/cyto.990070104</identifier><identifier>PMID: 2868850</identifier><identifier>CODEN: CYTODQ</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Alkaloids - pharmacology ; Animals ; Benzimidazoles - pharmacology ; Biological and medical sciences ; Cell structures and functions ; Cells, Cultured ; Computers ; Cytoplasmic microtubule complex ; Cytoskeleton - drug effects ; Cytoskeleton, cytoplasm. Intracellular movements ; Fourier Analysis ; Fourier analysis of cell shape ; Fundamental and applied biological sciences. Psychology ; Histocytochemistry ; Mathematics ; Mice ; Mice, Inbred C3H ; Microscopy, Phase-Contrast ; Microtubules - drug effects ; MO mouse cells ; Molecular and cellular biology ; Nocodazole ; optical diffractometry ; Paclitaxel ; Photomicrography - methods</subject><ispartof>Cytometry (New York, N.Y.), 1986-01, Vol.7 (1), p.18-24</ispartof><rights>Copyright © 1986 Wiley‐Liss, Inc.</rights><rights>1986 INIST-CNRS</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4094-781382dd6416575aa95ec7758ec0207d5184071dc8987a5a0cfc94bf774d98053</citedby><cites>FETCH-LOGICAL-c4094-781382dd6416575aa95ec7758ec0207d5184071dc8987a5a0cfc94bf774d98053</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8740968$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2868850$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mareel, Marc M.</creatorcontrib><creatorcontrib>De Bruyne, Georges K.</creatorcontrib><creatorcontrib>Ostrowski, Kazimierz</creatorcontrib><creatorcontrib>Rozycka, Monika</creatorcontrib><creatorcontrib>Strojny, Pawel</creatorcontrib><creatorcontrib>Dziedzic‐Goclawska, Anna</creatorcontrib><creatorcontrib>Lenczowski, Stanislaw</creatorcontrib><creatorcontrib>Grzesik, Wojcech</creatorcontrib><creatorcontrib>Kieler, Jorgen</creatorcontrib><title>Numerical evaluation of changes in the cytoplasmic microtubule complex of C3H mouse cells by optical diffractometry and of changes in cell shape by fourier analysis</title><title>Cytometry (New York, N.Y.)</title><addtitle>Cytometry</addtitle><description>Mo mouse cells in culture on glass were treated with taxol, or nocodazole, or incubated at 4°C to alter their cytoplasmic microtubule complex (CMTC). From each treated group and from an untreated group, 30 cells stained with an antiserum against tubulin, were photographed under the photomicroscope, and negatives were analysed by optical diffractometry. Differences between groups of cells were tested by variance analysis. Phasecontrast micrographs of the same cells were used for Fourier analysis of cell shape. Both types of analyses provided numerical objective data about changes in the CMTC and in cell shape that were typical for the kind of treatment. We conclude that optical diffratometry of immunostained cells and Fourier analysis of cell shape are complementary to photomicroscopy for the study of the CMTC in cell populations cultured on an artificial substrate.</description><subject>Alkaloids - pharmacology</subject><subject>Animals</subject><subject>Benzimidazoles - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Cell structures and functions</subject><subject>Cells, Cultured</subject><subject>Computers</subject><subject>Cytoplasmic microtubule complex</subject><subject>Cytoskeleton - drug effects</subject><subject>Cytoskeleton, cytoplasm. Intracellular movements</subject><subject>Fourier Analysis</subject><subject>Fourier analysis of cell shape</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Histocytochemistry</subject><subject>Mathematics</subject><subject>Mice</subject><subject>Mice, Inbred C3H</subject><subject>Microscopy, Phase-Contrast</subject><subject>Microtubules - drug effects</subject><subject>MO mouse cells</subject><subject>Molecular and cellular biology</subject><subject>Nocodazole</subject><subject>optical diffractometry</subject><subject>Paclitaxel</subject><subject>Photomicrography - methods</subject><issn>0196-4763</issn><issn>1097-0320</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhq0K1C5tr9wq-YC4ZRknTmwfq1WhSBW9tIeeolnH6Ro5cbATaP4PPxSHXS1SLxwsSzPPOx_vEPKewZoB5J_0PPq1UgACGPATsmKgRAZFDm_ICpiqMi6q4oy8i_E7AKiKF6fkNJeVlCWsyO9vU2eC1eio-YluwtH6nvqW6h32zyZS29NxZ-jSZnAYO6tpesGP03ZyKe67wZmXRbEpbmnnp5iCxrlItzP1w_i3dGPbNqAefWfGMFPsm1ctFgWNOxzMImv9FKwJiUM3RxsvyNsWXTSXh_-cPH6-edjcZnf3X75uru8yzUHxTEhWyLxpKs6qUpSIqjRaiFIaDTmIpmSSg2CNlkoKLBF0qxXftkLwRkkoi3PycV93CP7HZOJYdzYuo2Fv0mK1qARLpi_geg8mI2IMpq2HYDsMc82gXs5SL37Vx7MkwdWh8rTtTHPED3dI-Q-HPMZkWDKr1zYeMSnSgpVMmNpjv6wz83-a1punh_t_I_wBVlCpSw</recordid><startdate>198601</startdate><enddate>198601</enddate><creator>Mareel, Marc M.</creator><creator>De Bruyne, Georges K.</creator><creator>Ostrowski, Kazimierz</creator><creator>Rozycka, Monika</creator><creator>Strojny, Pawel</creator><creator>Dziedzic‐Goclawska, Anna</creator><creator>Lenczowski, Stanislaw</creator><creator>Grzesik, Wojcech</creator><creator>Kieler, Jorgen</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198601</creationdate><title>Numerical evaluation of changes in the cytoplasmic microtubule complex of C3H mouse cells by optical diffractometry and of changes in cell shape by fourier analysis</title><author>Mareel, Marc M. ; De Bruyne, Georges K. ; Ostrowski, Kazimierz ; Rozycka, Monika ; Strojny, Pawel ; Dziedzic‐Goclawska, Anna ; Lenczowski, Stanislaw ; Grzesik, Wojcech ; Kieler, Jorgen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4094-781382dd6416575aa95ec7758ec0207d5184071dc8987a5a0cfc94bf774d98053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Alkaloids - pharmacology</topic><topic>Animals</topic><topic>Benzimidazoles - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Cell structures and functions</topic><topic>Cells, Cultured</topic><topic>Computers</topic><topic>Cytoplasmic microtubule complex</topic><topic>Cytoskeleton - drug effects</topic><topic>Cytoskeleton, cytoplasm. Intracellular movements</topic><topic>Fourier Analysis</topic><topic>Fourier analysis of cell shape</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Histocytochemistry</topic><topic>Mathematics</topic><topic>Mice</topic><topic>Mice, Inbred C3H</topic><topic>Microscopy, Phase-Contrast</topic><topic>Microtubules - drug effects</topic><topic>MO mouse cells</topic><topic>Molecular and cellular biology</topic><topic>Nocodazole</topic><topic>optical diffractometry</topic><topic>Paclitaxel</topic><topic>Photomicrography - methods</topic><toplevel>online_resources</toplevel><creatorcontrib>Mareel, Marc M.</creatorcontrib><creatorcontrib>De Bruyne, Georges K.</creatorcontrib><creatorcontrib>Ostrowski, Kazimierz</creatorcontrib><creatorcontrib>Rozycka, Monika</creatorcontrib><creatorcontrib>Strojny, Pawel</creatorcontrib><creatorcontrib>Dziedzic‐Goclawska, Anna</creatorcontrib><creatorcontrib>Lenczowski, Stanislaw</creatorcontrib><creatorcontrib>Grzesik, Wojcech</creatorcontrib><creatorcontrib>Kieler, Jorgen</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cytometry (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mareel, Marc M.</au><au>De Bruyne, Georges K.</au><au>Ostrowski, Kazimierz</au><au>Rozycka, Monika</au><au>Strojny, Pawel</au><au>Dziedzic‐Goclawska, Anna</au><au>Lenczowski, Stanislaw</au><au>Grzesik, Wojcech</au><au>Kieler, Jorgen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Numerical evaluation of changes in the cytoplasmic microtubule complex of C3H mouse cells by optical diffractometry and of changes in cell shape by fourier analysis</atitle><jtitle>Cytometry (New York, N.Y.)</jtitle><addtitle>Cytometry</addtitle><date>1986-01</date><risdate>1986</risdate><volume>7</volume><issue>1</issue><spage>18</spage><epage>24</epage><pages>18-24</pages><issn>0196-4763</issn><eissn>1097-0320</eissn><coden>CYTODQ</coden><abstract>Mo mouse cells in culture on glass were treated with taxol, or nocodazole, or incubated at 4°C to alter their cytoplasmic microtubule complex (CMTC). From each treated group and from an untreated group, 30 cells stained with an antiserum against tubulin, were photographed under the photomicroscope, and negatives were analysed by optical diffractometry. Differences between groups of cells were tested by variance analysis. Phasecontrast micrographs of the same cells were used for Fourier analysis of cell shape. Both types of analyses provided numerical objective data about changes in the CMTC and in cell shape that were typical for the kind of treatment. We conclude that optical diffratometry of immunostained cells and Fourier analysis of cell shape are complementary to photomicroscopy for the study of the CMTC in cell populations cultured on an artificial substrate.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>2868850</pmid><doi>10.1002/cyto.990070104</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Cytometry (New York, N.Y.), 1986-01, Vol.7 (1), p.18-24 |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Alkaloids - pharmacology Animals Benzimidazoles - pharmacology Biological and medical sciences Cell structures and functions Cells, Cultured Computers Cytoplasmic microtubule complex Cytoskeleton - drug effects Cytoskeleton, cytoplasm. Intracellular movements Fourier Analysis Fourier analysis of cell shape Fundamental and applied biological sciences. Psychology Histocytochemistry Mathematics Mice Mice, Inbred C3H Microscopy, Phase-Contrast Microtubules - drug effects MO mouse cells Molecular and cellular biology Nocodazole optical diffractometry Paclitaxel Photomicrography - methods |
| title | Numerical evaluation of changes in the cytoplasmic microtubule complex of C3H mouse cells by optical diffractometry and of changes in cell shape by fourier analysis |
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