Western blot assay for prostate-specific membrane antigen in serum of prostate cancer patients
There is a need for the development of new diagnostic tools for the early detection of prostate cancer. A candidate molecule for a new screening test is a prostate‐specific membrane antigen (PSM) recognized by the monoclonal antibody 7E11.C5. We carried out studies aimed at identifying PSM in the se...
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| Veröffentlicht in: | The Prostate 1994-10, Vol.25 (4), p.219-223 |
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| container_title | The Prostate |
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| creator | Rochon, Yvan P. Horoszewicz, Julius S. Boynton, Alton L. Holmes, Eric H. Barren III, Robert J. Erickson, Sheila J. Kenny, Gerald M. Murphy, Gerald P. |
| description | There is a need for the development of new diagnostic tools for the early detection of prostate cancer. A candidate molecule for a new screening test is a prostate‐specific membrane antigen (PSM) recognized by the monoclonal antibody 7E11.C5. We carried out studies aimed at identifying PSM in the serum of normal and benign prostatic hyperplasia (BPH) donors and patients with adenocarcinoma of the prostate, in order to judge whether the development of a serum assay using this marker was feasible. By Western blotting, we found significant levels of PSM in serum samples from prostatic cancer patients, in the seminal fluid of pooled normal donors, in BPH patients, and in normal male sera. Similar to prostate‐specific antigen (PSA), PSM was present in seminal plasma in higher concentrations than in serum, and PSM levels in prostatic cancer patients were significantly higher than in normal controls. These data suggest that the development of an assay utilizing the PSM and new monoclonal antibodies directed against the antigen, could provide a feasible test for prostatic cancers. © 1994 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/pros.2990250408 |
| format | Article |
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A candidate molecule for a new screening test is a prostate‐specific membrane antigen (PSM) recognized by the monoclonal antibody 7E11.C5. We carried out studies aimed at identifying PSM in the serum of normal and benign prostatic hyperplasia (BPH) donors and patients with adenocarcinoma of the prostate, in order to judge whether the development of a serum assay using this marker was feasible. By Western blotting, we found significant levels of PSM in serum samples from prostatic cancer patients, in the seminal fluid of pooled normal donors, in BPH patients, and in normal male sera. Similar to prostate‐specific antigen (PSA), PSM was present in seminal plasma in higher concentrations than in serum, and PSM levels in prostatic cancer patients were significantly higher than in normal controls. These data suggest that the development of an assay utilizing the PSM and new monoclonal antibodies directed against the antigen, could provide a feasible test for prostatic cancers. © 1994 Wiley‐Liss, Inc.</description><identifier>ISSN: 0270-4137</identifier><identifier>EISSN: 1097-0045</identifier><identifier>DOI: 10.1002/pros.2990250408</identifier><identifier>PMID: 8084837</identifier><identifier>CODEN: PRSTDS</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Adenocarcinoma - blood ; Adenocarcinoma - diagnosis ; Adenocarcinoma - immunology ; Antigens, Neoplasm - analysis ; Antigens, Neoplasm - blood ; Antigens, Surface - analysis ; Antigens, Surface - blood ; Binding, Competitive ; Biological and medical sciences ; Blotting, Western ; Cell Line ; Enzyme-Linked Immunosorbent Assay ; Glutamate Carboxypeptidase II ; Humans ; Male ; Medical sciences ; monoclonal antibodies ; Nephrology. Urinary tract diseases ; prostatic adenocarcinoma ; Prostatic Neoplasms - blood ; Prostatic Neoplasms - diagnosis ; Prostatic Neoplasms - immunology ; PSM ; Semen - chemistry ; serodiagnosis ; Tumors of the urinary system ; Urinary tract. Prostate gland ; Western blotting</subject><ispartof>The Prostate, 1994-10, Vol.25 (4), p.219-223</ispartof><rights>Copyright © 1994 Wiley‐Liss, Inc., A Wiley Company</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4118-ec62866ed3507c65b4455f63a04744011f43c26047df88a86ec7fb01183574ea3</citedby><cites>FETCH-LOGICAL-c4118-ec62866ed3507c65b4455f63a04744011f43c26047df88a86ec7fb01183574ea3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fpros.2990250408$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fpros.2990250408$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3297695$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8084837$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rochon, Yvan P.</creatorcontrib><creatorcontrib>Horoszewicz, Julius S.</creatorcontrib><creatorcontrib>Boynton, Alton L.</creatorcontrib><creatorcontrib>Holmes, Eric H.</creatorcontrib><creatorcontrib>Barren III, Robert J.</creatorcontrib><creatorcontrib>Erickson, Sheila J.</creatorcontrib><creatorcontrib>Kenny, Gerald M.</creatorcontrib><creatorcontrib>Murphy, Gerald P.</creatorcontrib><title>Western blot assay for prostate-specific membrane antigen in serum of prostate cancer patients</title><title>The Prostate</title><addtitle>Prostate</addtitle><description>There is a need for the development of new diagnostic tools for the early detection of prostate cancer. A candidate molecule for a new screening test is a prostate‐specific membrane antigen (PSM) recognized by the monoclonal antibody 7E11.C5. We carried out studies aimed at identifying PSM in the serum of normal and benign prostatic hyperplasia (BPH) donors and patients with adenocarcinoma of the prostate, in order to judge whether the development of a serum assay using this marker was feasible. By Western blotting, we found significant levels of PSM in serum samples from prostatic cancer patients, in the seminal fluid of pooled normal donors, in BPH patients, and in normal male sera. Similar to prostate‐specific antigen (PSA), PSM was present in seminal plasma in higher concentrations than in serum, and PSM levels in prostatic cancer patients were significantly higher than in normal controls. These data suggest that the development of an assay utilizing the PSM and new monoclonal antibodies directed against the antigen, could provide a feasible test for prostatic cancers. © 1994 Wiley‐Liss, Inc.</description><subject>Adenocarcinoma - blood</subject><subject>Adenocarcinoma - diagnosis</subject><subject>Adenocarcinoma - immunology</subject><subject>Antigens, Neoplasm - analysis</subject><subject>Antigens, Neoplasm - blood</subject><subject>Antigens, Surface - analysis</subject><subject>Antigens, Surface - blood</subject><subject>Binding, Competitive</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Cell Line</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Glutamate Carboxypeptidase II</subject><subject>Humans</subject><subject>Male</subject><subject>Medical sciences</subject><subject>monoclonal antibodies</subject><subject>Nephrology. Urinary tract diseases</subject><subject>prostatic adenocarcinoma</subject><subject>Prostatic Neoplasms - blood</subject><subject>Prostatic Neoplasms - diagnosis</subject><subject>Prostatic Neoplasms - immunology</subject><subject>PSM</subject><subject>Semen - chemistry</subject><subject>serodiagnosis</subject><subject>Tumors of the urinary system</subject><subject>Urinary tract. Prostate gland</subject><subject>Western blotting</subject><issn>0270-4137</issn><issn>1097-0045</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1vEzEQxS0EKqFw5oTkA-K27Xj9ueJEI2iQqha1RZF6wPI6Y2TYj2BvBPnvcZQoiFNPlj2_N_PmmZDXDM4YQH2-TmM-q5sGagkCzBMyY9DoCkDIp2QGtYZKMK6fkxc5_wAoGqhPyIkBIwzXM_JtiXnCNNC2GyfqcnZbGsZEd30nN2GV1-hjiJ722LfJDUjdMMXvONA40Ixp09MxHHHq3eCxyN0UcZjyS_IsuC7jq8N5Sr5--ng_X1RXN5ef5x-uKi8YMxV6VRulcMUlaK9kK4SUQXEHQgsBjAXBfa3KbRWMcUah16Et74ZLLdDxU_Ju37cY-bUpK9k-Zo9dVwyPm2y10gyMYQU834O-OM4Jg12n2Lu0tQzsLlG7W8X-S7Qo3hxab9oeV0f-EGGpvz3UXfauCyUjH_MR43WjVSML9n6P_Y4dbh-bar_c3tz9Z6Laq2P5rT9HtUs_rdJcS7u8vrTLxUJf3M4f7AP_Cz6An8s</recordid><startdate>199410</startdate><enddate>199410</enddate><creator>Rochon, Yvan P.</creator><creator>Horoszewicz, Julius S.</creator><creator>Boynton, Alton L.</creator><creator>Holmes, Eric H.</creator><creator>Barren III, Robert J.</creator><creator>Erickson, Sheila J.</creator><creator>Kenny, Gerald M.</creator><creator>Murphy, Gerald P.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199410</creationdate><title>Western blot assay for prostate-specific membrane antigen in serum of prostate cancer patients</title><author>Rochon, Yvan P. ; Horoszewicz, Julius S. ; Boynton, Alton L. ; Holmes, Eric H. ; Barren III, Robert J. ; Erickson, Sheila J. ; Kenny, Gerald M. ; Murphy, Gerald P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4118-ec62866ed3507c65b4455f63a04744011f43c26047df88a86ec7fb01183574ea3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Adenocarcinoma - blood</topic><topic>Adenocarcinoma - diagnosis</topic><topic>Adenocarcinoma - immunology</topic><topic>Antigens, Neoplasm - analysis</topic><topic>Antigens, Neoplasm - blood</topic><topic>Antigens, Surface - analysis</topic><topic>Antigens, Surface - blood</topic><topic>Binding, Competitive</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Cell Line</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Glutamate Carboxypeptidase II</topic><topic>Humans</topic><topic>Male</topic><topic>Medical sciences</topic><topic>monoclonal antibodies</topic><topic>Nephrology. Urinary tract diseases</topic><topic>prostatic adenocarcinoma</topic><topic>Prostatic Neoplasms - blood</topic><topic>Prostatic Neoplasms - diagnosis</topic><topic>Prostatic Neoplasms - immunology</topic><topic>PSM</topic><topic>Semen - chemistry</topic><topic>serodiagnosis</topic><topic>Tumors of the urinary system</topic><topic>Urinary tract. 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A candidate molecule for a new screening test is a prostate‐specific membrane antigen (PSM) recognized by the monoclonal antibody 7E11.C5. We carried out studies aimed at identifying PSM in the serum of normal and benign prostatic hyperplasia (BPH) donors and patients with adenocarcinoma of the prostate, in order to judge whether the development of a serum assay using this marker was feasible. By Western blotting, we found significant levels of PSM in serum samples from prostatic cancer patients, in the seminal fluid of pooled normal donors, in BPH patients, and in normal male sera. Similar to prostate‐specific antigen (PSA), PSM was present in seminal plasma in higher concentrations than in serum, and PSM levels in prostatic cancer patients were significantly higher than in normal controls. These data suggest that the development of an assay utilizing the PSM and new monoclonal antibodies directed against the antigen, could provide a feasible test for prostatic cancers. © 1994 Wiley‐Liss, Inc.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>8084837</pmid><doi>10.1002/pros.2990250408</doi><tpages>5</tpages></addata></record> |
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| ispartof | The Prostate, 1994-10, Vol.25 (4), p.219-223 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library All Journals |
| subjects | Adenocarcinoma - blood Adenocarcinoma - diagnosis Adenocarcinoma - immunology Antigens, Neoplasm - analysis Antigens, Neoplasm - blood Antigens, Surface - analysis Antigens, Surface - blood Binding, Competitive Biological and medical sciences Blotting, Western Cell Line Enzyme-Linked Immunosorbent Assay Glutamate Carboxypeptidase II Humans Male Medical sciences monoclonal antibodies Nephrology. Urinary tract diseases prostatic adenocarcinoma Prostatic Neoplasms - blood Prostatic Neoplasms - diagnosis Prostatic Neoplasms - immunology PSM Semen - chemistry serodiagnosis Tumors of the urinary system Urinary tract. Prostate gland Western blotting |
| title | Western blot assay for prostate-specific membrane antigen in serum of prostate cancer patients |
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