Solid-phase techniques in blood transfusion serology

For nearly a century, erythrocyte agglutination has persisted as the most widely used method for the demonstration of antigen-antibody reaction in immunohematology. So far, no other system has been developed which can match its simplicity, versatility, and general reliability. The major disadvantage...

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Veröffentlicht in:CRC critical reviews in clinical laboratory sciences 1986, Vol.22 (4), p.317-432
Hauptverfasser: BECK, M. L, PLAPP, F. V, SINOR, L. T, RACHEL, J. M, BLUMBERG, N
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container_issue 4
container_start_page 317
container_title CRC critical reviews in clinical laboratory sciences
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creator BECK, M. L
PLAPP, F. V
SINOR, L. T
RACHEL, J. M
BLUMBERG, N
description For nearly a century, erythrocyte agglutination has persisted as the most widely used method for the demonstration of antigen-antibody reaction in immunohematology. So far, no other system has been developed which can match its simplicity, versatility, and general reliability. The major disadvantage of agglutination reactions is the lack of an objective endpoint, which has severely hindered attempts to automate routine pretransfusion tests. To overcome this problem, we have designed a series of solid-phase assays for ABO and Rh grouping, antibody screening, compatibility, and hepatitis tests. Each of these solid-phase assays shares a common endpoint of red cell adherence, which is easily interpreted visually or spectrophotometrically. Computer interface permits the automatic interpretation and recording of results. We believe this solid-phase system should finally bring the blood bank laboratory into the age of automation.
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identifier ISSN: 0590-8191
ispartof CRC critical reviews in clinical laboratory sciences, 1986, Vol.22 (4), p.317-432
issn 0590-8191
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language eng
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source Taylor & Francis; MEDLINE; Taylor & Francis Medical Library - CRKN
subjects ABO Blood-Group System - immunology
Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy
Antibodies, Anti-Idiotypic - immunology
Antibodies, Monoclonal
Biological and medical sciences
Blood Grouping and Crossmatching - methods
Blood Platelets - immunology
Blood Transfusion
Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis
Hemagglutination Tests - methods
Hepatitis B Surface Antigens - analysis
Humans
Immune Adherence Reaction
Isoantibodies - analysis
Medical sciences
Rh-Hr Blood-Group System - immunology
Saliva - immunology
Transfusions. Complications. Transfusion reactions. Cell and gene therapy
title Solid-phase techniques in blood transfusion serology
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