Prevalence of hepatitis-C virus RNA in serum and throat washings of children with chronic hepatitis
Serum samples from 46 children with chronic and probably transfusion acquired hepatitis were tested for the presence of hepatitis C virus (HCV) RNA by a “nested” polymerase chain reaction (PCR) assay, to judge a possible risk of HCV transmission from these patients. In 73% of the samples, viral RNA...
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| Veröffentlicht in: | Journal of medical virology 1994-06, Vol.43 (2), p.143-147 |
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| creator | Puchhammer-Stöckl, Elisabeth Mor, Wolfgang Kundi, Michael Heinz, Franz-Xaver Hofmann, Hanns Kunz, Christian |
| description | Serum samples from 46 children with chronic and probably transfusion acquired hepatitis were tested for the presence of hepatitis C virus (HCV) RNA by a “nested” polymerase chain reaction (PCR) assay, to judge a possible risk of HCV transmission from these patients. In 73% of the samples, viral RNA was detected, indicating a high virus prevalence in this patient group. High titers of HCV‐RNA were observed in some sera as shown by the detection of virus in some samples even at dilutions of 10−3. Comparison of simultaneously obtained PCR results and ALT values revealed no significant correlation between virus presence in serum and higher ALT levels. It was, however, shown that unusually high ALT values may reflect a high titer of viral RNA in serum. To investigate the prevalence of viral RNA in saliva, which could be a vehicle of virus transmission, 35 throat washing samples from the HCV‐infected children were screened by PCR. Using three different sample preparation procedures, 20% of the throat washings were found to be positive for HCV‐RNA. This indicates a prevalence of virus in this fluid lower than that reported previously. © 1994 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/jmv.1890430208 |
| format | Article |
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In 73% of the samples, viral RNA was detected, indicating a high virus prevalence in this patient group. High titers of HCV‐RNA were observed in some sera as shown by the detection of virus in some samples even at dilutions of 10−3. Comparison of simultaneously obtained PCR results and ALT values revealed no significant correlation between virus presence in serum and higher ALT levels. It was, however, shown that unusually high ALT values may reflect a high titer of viral RNA in serum. To investigate the prevalence of viral RNA in saliva, which could be a vehicle of virus transmission, 35 throat washing samples from the HCV‐infected children were screened by PCR. Using three different sample preparation procedures, 20% of the throat washings were found to be positive for HCV‐RNA. This indicates a prevalence of virus in this fluid lower than that reported previously. © 1994 Wiley‐Liss, Inc.</description><identifier>ISSN: 0146-6615</identifier><identifier>EISSN: 1096-9071</identifier><identifier>DOI: 10.1002/jmv.1890430208</identifier><identifier>PMID: 8083661</identifier><identifier>CODEN: JMVIDB</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Alanine Transaminase - blood ; Base Sequence ; Biological and medical sciences ; Child ; Chronic Disease ; chronic post-transfusion hepatitis ; Female ; Hepacivirus - isolation & purification ; Hepatitis C - blood ; Hepatitis C - microbiology ; hepatitis C virus ; Human viral diseases ; Humans ; Infectious diseases ; Male ; Medical sciences ; Molecular Sequence Data ; Oncology Service, Hospital ; Pharynx - microbiology ; Polymerase Chain Reaction ; Prevalence ; RNA, Viral - analysis ; RNA, Viral - blood ; Saliva - microbiology ; throat washings ; Viral diseases ; Viral hepatitis</subject><ispartof>Journal of medical virology, 1994-06, Vol.43 (2), p.143-147</ispartof><rights>Copyright © 1994 Wiley‐Liss, Inc., A Wiley Company</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4388-311c998ef912f0ce67453aff8918331483c50eb7eb5abde5212f34c2c245c23b3</citedby><cites>FETCH-LOGICAL-c4388-311c998ef912f0ce67453aff8918331483c50eb7eb5abde5212f34c2c245c23b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjmv.1890430208$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjmv.1890430208$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4135439$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8083661$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Puchhammer-Stöckl, Elisabeth</creatorcontrib><creatorcontrib>Mor, Wolfgang</creatorcontrib><creatorcontrib>Kundi, Michael</creatorcontrib><creatorcontrib>Heinz, Franz-Xaver</creatorcontrib><creatorcontrib>Hofmann, Hanns</creatorcontrib><creatorcontrib>Kunz, Christian</creatorcontrib><title>Prevalence of hepatitis-C virus RNA in serum and throat washings of children with chronic hepatitis</title><title>Journal of medical virology</title><addtitle>J. Med. Virol</addtitle><description>Serum samples from 46 children with chronic and probably transfusion acquired hepatitis were tested for the presence of hepatitis C virus (HCV) RNA by a “nested” polymerase chain reaction (PCR) assay, to judge a possible risk of HCV transmission from these patients. In 73% of the samples, viral RNA was detected, indicating a high virus prevalence in this patient group. High titers of HCV‐RNA were observed in some sera as shown by the detection of virus in some samples even at dilutions of 10−3. Comparison of simultaneously obtained PCR results and ALT values revealed no significant correlation between virus presence in serum and higher ALT levels. It was, however, shown that unusually high ALT values may reflect a high titer of viral RNA in serum. To investigate the prevalence of viral RNA in saliva, which could be a vehicle of virus transmission, 35 throat washing samples from the HCV‐infected children were screened by PCR. Using three different sample preparation procedures, 20% of the throat washings were found to be positive for HCV‐RNA. This indicates a prevalence of virus in this fluid lower than that reported previously. © 1994 Wiley‐Liss, Inc.</description><subject>Alanine Transaminase - blood</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Child</subject><subject>Chronic Disease</subject><subject>chronic post-transfusion hepatitis</subject><subject>Female</subject><subject>Hepacivirus - isolation & purification</subject><subject>Hepatitis C - blood</subject><subject>Hepatitis C - microbiology</subject><subject>hepatitis C virus</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Molecular Sequence Data</subject><subject>Oncology Service, Hospital</subject><subject>Pharynx - microbiology</subject><subject>Polymerase Chain Reaction</subject><subject>Prevalence</subject><subject>RNA, Viral - analysis</subject><subject>RNA, Viral - blood</subject><subject>Saliva - microbiology</subject><subject>throat washings</subject><subject>Viral diseases</subject><subject>Viral hepatitis</subject><issn>0146-6615</issn><issn>1096-9071</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1vEzEQxS0EKqFw5YbkA-ptw_hjvfaximgplBYhKNwsrzPLuuxHsHeT9r9no0Speupp5Jnfe896hLxlMGcA_MNtu54zbUAK4KCfkRkDozIDBXtOZsCkypRi-UvyKqVbANCG8yNypEGLaT8j_lvEtWuw80j7ita4ckMYQsoWdB3imOj3q1MaOpowji113ZIOdezdQDcu1aH7k7YqX4dmGbGjmzDU0yv2XfAPXq_Ji8o1Cd_s5zH5efbxx-JTdnl9frE4vcy8FFpngjFvjMbKMF6BR1XIXLiq0oZpIZjUwueAZYFl7sol5nzChPTcc5l7LkpxTE52vqvY_xsxDbYNyWPTuA77MdlCFSCZhCdBpgwTuTQTON-BPvYpRazsKobWxXvLwG7rt1P99qH-SfBu7zyWLS4P-L7v6f5-f3fJu6aKrvMhHTC5jRXbXLPDNqHB-ydC7eevN4--kO20IQ14d9C6-NeqQhS5_XV1btWN-i2LL9rm4j_FFqyb</recordid><startdate>199406</startdate><enddate>199406</enddate><creator>Puchhammer-Stöckl, Elisabeth</creator><creator>Mor, Wolfgang</creator><creator>Kundi, Michael</creator><creator>Heinz, Franz-Xaver</creator><creator>Hofmann, Hanns</creator><creator>Kunz, Christian</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>199406</creationdate><title>Prevalence of hepatitis-C virus RNA in serum and throat washings of children with chronic hepatitis</title><author>Puchhammer-Stöckl, Elisabeth ; Mor, Wolfgang ; Kundi, Michael ; Heinz, Franz-Xaver ; Hofmann, Hanns ; Kunz, Christian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4388-311c998ef912f0ce67453aff8918331483c50eb7eb5abde5212f34c2c245c23b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Alanine Transaminase - blood</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Child</topic><topic>Chronic Disease</topic><topic>chronic post-transfusion hepatitis</topic><topic>Female</topic><topic>Hepacivirus - isolation & purification</topic><topic>Hepatitis C - blood</topic><topic>Hepatitis C - microbiology</topic><topic>hepatitis C virus</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Molecular Sequence Data</topic><topic>Oncology Service, Hospital</topic><topic>Pharynx - microbiology</topic><topic>Polymerase Chain Reaction</topic><topic>Prevalence</topic><topic>RNA, Viral - analysis</topic><topic>RNA, Viral - blood</topic><topic>Saliva - microbiology</topic><topic>throat washings</topic><topic>Viral diseases</topic><topic>Viral hepatitis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Puchhammer-Stöckl, Elisabeth</creatorcontrib><creatorcontrib>Mor, Wolfgang</creatorcontrib><creatorcontrib>Kundi, Michael</creatorcontrib><creatorcontrib>Heinz, Franz-Xaver</creatorcontrib><creatorcontrib>Hofmann, Hanns</creatorcontrib><creatorcontrib>Kunz, Christian</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of medical virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Puchhammer-Stöckl, Elisabeth</au><au>Mor, Wolfgang</au><au>Kundi, Michael</au><au>Heinz, Franz-Xaver</au><au>Hofmann, Hanns</au><au>Kunz, Christian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prevalence of hepatitis-C virus RNA in serum and throat washings of children with chronic hepatitis</atitle><jtitle>Journal of medical virology</jtitle><addtitle>J. Med. Virol</addtitle><date>1994-06</date><risdate>1994</risdate><volume>43</volume><issue>2</issue><spage>143</spage><epage>147</epage><pages>143-147</pages><issn>0146-6615</issn><eissn>1096-9071</eissn><coden>JMVIDB</coden><abstract>Serum samples from 46 children with chronic and probably transfusion acquired hepatitis were tested for the presence of hepatitis C virus (HCV) RNA by a “nested” polymerase chain reaction (PCR) assay, to judge a possible risk of HCV transmission from these patients. In 73% of the samples, viral RNA was detected, indicating a high virus prevalence in this patient group. High titers of HCV‐RNA were observed in some sera as shown by the detection of virus in some samples even at dilutions of 10−3. Comparison of simultaneously obtained PCR results and ALT values revealed no significant correlation between virus presence in serum and higher ALT levels. It was, however, shown that unusually high ALT values may reflect a high titer of viral RNA in serum. To investigate the prevalence of viral RNA in saliva, which could be a vehicle of virus transmission, 35 throat washing samples from the HCV‐infected children were screened by PCR. Using three different sample preparation procedures, 20% of the throat washings were found to be positive for HCV‐RNA. This indicates a prevalence of virus in this fluid lower than that reported previously. © 1994 Wiley‐Liss, Inc.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>8083661</pmid><doi>10.1002/jmv.1890430208</doi><tpages>5</tpages></addata></record> |
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| subjects | Alanine Transaminase - blood Base Sequence Biological and medical sciences Child Chronic Disease chronic post-transfusion hepatitis Female Hepacivirus - isolation & purification Hepatitis C - blood Hepatitis C - microbiology hepatitis C virus Human viral diseases Humans Infectious diseases Male Medical sciences Molecular Sequence Data Oncology Service, Hospital Pharynx - microbiology Polymerase Chain Reaction Prevalence RNA, Viral - analysis RNA, Viral - blood Saliva - microbiology throat washings Viral diseases Viral hepatitis |
| title | Prevalence of hepatitis-C virus RNA in serum and throat washings of children with chronic hepatitis |
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