Supported phospholipid bilayers for two-dimensional protein crystallization
Phospholipid bilayers, supported on UV irradiated carbon shadowed nitro-cellulose electron microscope grids, have been used to induce two-dimensional crystal growth of IgE and IgG anti-DNP monoclonal antibodies. The UV irradiation renders the grids hydrophilic in a very uniform fashion and allows fo...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1986-01, Vol.134 (2), p.819-826 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Uzgiris, E.E. |
| description | Phospholipid bilayers, supported on UV irradiated carbon shadowed nitro-cellulose electron microscope grids, have been used to induce two-dimensional crystal growth of IgE and IgG anti-DNP monoclonal antibodies. The UV irradiation renders the grids hydrophilic in a very uniform fashion and allows for the transfer of phospholipid monolayers from an air/water interface in a sequential dipping procedure. The surface coverage achieved was nearly 100% as measured by antibody binding and by the formation of protein arrays on the bilayer covered grids. The supported bilayers appear to be stably held and are appropriate for slow binding conditions and long incubation times with low concentrations of binding protein. |
| doi_str_mv | 10.1016/S0006-291X(86)80494-6 |
| format | Article |
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The UV irradiation renders the grids hydrophilic in a very uniform fashion and allows for the transfer of phospholipid monolayers from an air/water interface in a sequential dipping procedure. The surface coverage achieved was nearly 100% as measured by antibody binding and by the formation of protein arrays on the bilayer covered grids. The supported bilayers appear to be stably held and are appropriate for slow binding conditions and long incubation times with low concentrations of binding protein.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/S0006-291X(86)80494-6</identifier><identifier>PMID: 3947352</identifier><identifier>CODEN: BBRCA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Animals ; Antibodies, Monoclonal ; Applied sciences ; Crystallization ; Dinitrophenols - immunology ; Exact sciences and technology ; Immunoglobulin E ; Immunoglobulin G ; Lipid Bilayers ; Mice ; Microscopy, Electron - methods ; Other techniques and industries ; Phospholipids ; Proteins</subject><ispartof>Biochemical and biophysical research communications, 1986-01, Vol.134 (2), p.819-826</ispartof><rights>1986 Academic Press, Inc. 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The supported bilayers appear to be stably held and are appropriate for slow binding conditions and long incubation times with low concentrations of binding protein.</description><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>Applied sciences</subject><subject>Crystallization</subject><subject>Dinitrophenols - immunology</subject><subject>Exact sciences and technology</subject><subject>Immunoglobulin E</subject><subject>Immunoglobulin G</subject><subject>Lipid Bilayers</subject><subject>Mice</subject><subject>Microscopy, Electron - methods</subject><subject>Other techniques and industries</subject><subject>Phospholipids</subject><subject>Proteins</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1P3DAQhq0KRBfKT0DKoULlEBgntmOfEEL9QCBxoJW4WY49UV1549TOUi2_vl52tVcO1hze5x2PHkLOKFxSoOLqCQBE3Sj6_EWKCwlMsVp8IAsKCuqGAjsgiz3ykRzn_AeAUibUETlqFeta3izI_dNqmmKa0VXT75jLC37yrup9MGtMuRpiquZ_sXZ-iWP2cTShmlKc0Y-VTes8mxD8q5lL8okcDiZkPN3NE_Lr29eftz_qh8fvd7c3D7VlnM-16ZSUpuWSKwZcdJyBbWnvwBrJpWsapAPnfVcSo5S1HXa8obx33HUMhGpPyPl2b7nj7wrzrJc-WwzBjBhXWZcmAOMbkG9Bm2LOCQc9Jb80aa0p6I1E_SZRbwxpKfSbRC1K72z3wapfotu3dtZK_nmXm2xNGJIZrc97TIKgVHYFu95iWGS8eEw6W4-jRecT2lm76N855D9Sio7Z</recordid><startdate>19860129</startdate><enddate>19860129</enddate><creator>Uzgiris, E.E.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19860129</creationdate><title>Supported phospholipid bilayers for two-dimensional protein crystallization</title><author>Uzgiris, E.E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c455t-a7988a3585940567540c31bd0ca858d22e1f55b7675a99cc7e75215bd5d740693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>Applied sciences</topic><topic>Crystallization</topic><topic>Dinitrophenols - immunology</topic><topic>Exact sciences and technology</topic><topic>Immunoglobulin E</topic><topic>Immunoglobulin G</topic><topic>Lipid Bilayers</topic><topic>Mice</topic><topic>Microscopy, Electron - methods</topic><topic>Other techniques and industries</topic><topic>Phospholipids</topic><topic>Proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Uzgiris, E.E.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Uzgiris, E.E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Supported phospholipid bilayers for two-dimensional protein crystallization</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1986-01-29</date><risdate>1986</risdate><volume>134</volume><issue>2</issue><spage>819</spage><epage>826</epage><pages>819-826</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>Phospholipid bilayers, supported on UV irradiated carbon shadowed nitro-cellulose electron microscope grids, have been used to induce two-dimensional crystal growth of IgE and IgG anti-DNP monoclonal antibodies. 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| ispartof | Biochemical and biophysical research communications, 1986-01, Vol.134 (2), p.819-826 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76700459 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Antibodies, Monoclonal Applied sciences Crystallization Dinitrophenols - immunology Exact sciences and technology Immunoglobulin E Immunoglobulin G Lipid Bilayers Mice Microscopy, Electron - methods Other techniques and industries Phospholipids Proteins |
| title | Supported phospholipid bilayers for two-dimensional protein crystallization |
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