Regulation of immunoglobulin synthesis by human T cell subsets as defined by anti-D44 monoclonal antibody within the CD4+ and CD8+ subpopulations
In our previous paper, we demonstrated that anti-D44 MAb can, in the presence of complement, eliminate all the allocytotoxicity generated during a mixed lymphocyte reaction without affecting the alloproliferative response. As approximately 70% of CD4+ cells and 30% of CD8+ will be stained with anti-...
Gespeichert in:
| Veröffentlicht in: | The Journal of immunology (1950) 1986-02, Vol.136 (4), p.1144-1149 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1149 |
|---|---|
| container_issue | 4 |
| container_start_page | 1144 |
| container_title | The Journal of immunology (1950) |
| container_volume | 136 |
| creator | Calvo, CF Bernard, A Huet, S Leroy, E Boumsell, L Senik, A |
| description | In our previous paper, we demonstrated that anti-D44 MAb can, in the presence of complement, eliminate all the allocytotoxicity generated during a mixed lymphocyte reaction without affecting the alloproliferative response. As approximately 70% of CD4+ cells and 30% of CD8+ will be stained with anti-D44 MAb, we researched the functional role of the D44+ and D44- cells in each of these T cell subsets in the PWM-induced antibody response. We found that most of the helper activity for immunoglobulin (Ig) synthesis was mediated by CD4+ D44+ lymphocytes and that virtually all the suppressive activity was mediated by CD8+ D44- lymphocytes. Surprisingly enough, we noticed that the low level of Ig synthesis induced in B cells by CD4+ D44- lymphocytes could be strongly amplified by the addition of radiosensitive CD8+ lymphocytes, suggesting coexisting opposite immunoregulatory functions within the CD8+ T cell subset. These results, together with previous data, indicate that anti-D44 MAb subdivides T cells into subpopulations with distinct functional repertoires: a CD4+ D44+ helper subpopulation, a CD8+ D44+ cytotoxic subpopulation, and a CD8+ D44- suppressor subpopulation. |
| doi_str_mv | 10.4049/jimmunol.136.4.1144 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_76699263</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>76699263</sourcerecordid><originalsourceid>FETCH-LOGICAL-c406t-991bf5ac07ec0b6365b06b972a060414a4be99be13a8989799ae5a7042010873</originalsourceid><addsrcrecordid>eNpNkcFu3CAQhlHVKt2kfYKqEoeqOUTeDjYGc4w2bRMpUqVo7whsvCbCsDW2rH2MvnFx1o16AjHffDD8CH0isKVAxbdn2_eTD25LCralW0IofYM2pCwhYwzYW7QByPOMcMbfo8sYnwGAQU4v0EUuirLk-Qb9eTKHyanRBo9Di8_Ggwt6ctbjePJjZ6KNWJ9wN_XK4z2ujXM4TjqaMWIVcWNa602zIMqPNrujFPfBh9oFr9zLmQ7NCc927JIzCfHujt6kQpM21c3iOobj-or4Ab1rlYvm47peof2P7_vdffb46-fD7vYxqymwMROC6LZUNXBTg2YFKzUwLXiu0oyUUEW1EUIbUqhKVIILoUypONAcCFS8uEJfz9rjEH5PJo6yt3EZTXkTpig5Y0LkrEhgcQbrIcQ4mFYeB9ur4SQJyCUH-S8HmXKQVC45pK7Pq37SvWlee9aPT_Uva13FWrl2UL628RXjFeNARMKuz1hnD91sByNjr5xLUiLnef7vwr8ByqEW</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>76699263</pqid></control><display><type>article</type><title>Regulation of immunoglobulin synthesis by human T cell subsets as defined by anti-D44 monoclonal antibody within the CD4+ and CD8+ subpopulations</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Calvo, CF ; Bernard, A ; Huet, S ; Leroy, E ; Boumsell, L ; Senik, A</creator><creatorcontrib>Calvo, CF ; Bernard, A ; Huet, S ; Leroy, E ; Boumsell, L ; Senik, A</creatorcontrib><description>In our previous paper, we demonstrated that anti-D44 MAb can, in the presence of complement, eliminate all the allocytotoxicity generated during a mixed lymphocyte reaction without affecting the alloproliferative response. As approximately 70% of CD4+ cells and 30% of CD8+ will be stained with anti-D44 MAb, we researched the functional role of the D44+ and D44- cells in each of these T cell subsets in the PWM-induced antibody response. We found that most of the helper activity for immunoglobulin (Ig) synthesis was mediated by CD4+ D44+ lymphocytes and that virtually all the suppressive activity was mediated by CD8+ D44- lymphocytes. Surprisingly enough, we noticed that the low level of Ig synthesis induced in B cells by CD4+ D44- lymphocytes could be strongly amplified by the addition of radiosensitive CD8+ lymphocytes, suggesting coexisting opposite immunoregulatory functions within the CD8+ T cell subset. These results, together with previous data, indicate that anti-D44 MAb subdivides T cells into subpopulations with distinct functional repertoires: a CD4+ D44+ helper subpopulation, a CD8+ D44+ cytotoxic subpopulation, and a CD8+ D44- suppressor subpopulation.</description><identifier>ISSN: 0022-1767</identifier><identifier>EISSN: 1550-6606</identifier><identifier>DOI: 10.4049/jimmunol.136.4.1144</identifier><identifier>PMID: 2935572</identifier><identifier>CODEN: JOIMA3</identifier><language>eng</language><publisher>Bethesda, MD: Am Assoc Immnol</publisher><subject>Analysis of the immune response. Humoral and cellular immunity ; Antibodies, Monoclonal - immunology ; Antigen-Antibody Reactions ; Antigens, Differentiation, T-Lymphocyte ; Antigens, Surface - immunology ; B-Lymphocytes - immunology ; B-Lymphocytes - metabolism ; Biological and medical sciences ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Humans ; Immunobiology ; Immunoglobulins - biosynthesis ; Lymphocyte Activation ; Lymphocyte Cooperation ; Organs and cells involved in the immune response ; Phenotype ; Pokeweed Mitogens - pharmacology ; T-Lymphocytes - classification ; T-Lymphocytes - immunology ; T-Lymphocytes, Helper-Inducer - immunology</subject><ispartof>The Journal of immunology (1950), 1986-02, Vol.136 (4), p.1144-1149</ispartof><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-991bf5ac07ec0b6365b06b972a060414a4be99be13a8989799ae5a7042010873</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7867019$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2935572$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Calvo, CF</creatorcontrib><creatorcontrib>Bernard, A</creatorcontrib><creatorcontrib>Huet, S</creatorcontrib><creatorcontrib>Leroy, E</creatorcontrib><creatorcontrib>Boumsell, L</creatorcontrib><creatorcontrib>Senik, A</creatorcontrib><title>Regulation of immunoglobulin synthesis by human T cell subsets as defined by anti-D44 monoclonal antibody within the CD4+ and CD8+ subpopulations</title><title>The Journal of immunology (1950)</title><addtitle>J Immunol</addtitle><description>In our previous paper, we demonstrated that anti-D44 MAb can, in the presence of complement, eliminate all the allocytotoxicity generated during a mixed lymphocyte reaction without affecting the alloproliferative response. As approximately 70% of CD4+ cells and 30% of CD8+ will be stained with anti-D44 MAb, we researched the functional role of the D44+ and D44- cells in each of these T cell subsets in the PWM-induced antibody response. We found that most of the helper activity for immunoglobulin (Ig) synthesis was mediated by CD4+ D44+ lymphocytes and that virtually all the suppressive activity was mediated by CD8+ D44- lymphocytes. Surprisingly enough, we noticed that the low level of Ig synthesis induced in B cells by CD4+ D44- lymphocytes could be strongly amplified by the addition of radiosensitive CD8+ lymphocytes, suggesting coexisting opposite immunoregulatory functions within the CD8+ T cell subset. These results, together with previous data, indicate that anti-D44 MAb subdivides T cells into subpopulations with distinct functional repertoires: a CD4+ D44+ helper subpopulation, a CD8+ D44+ cytotoxic subpopulation, and a CD8+ D44- suppressor subpopulation.</description><subject>Analysis of the immune response. Humoral and cellular immunity</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antigen-Antibody Reactions</subject><subject>Antigens, Differentiation, T-Lymphocyte</subject><subject>Antigens, Surface - immunology</subject><subject>B-Lymphocytes - immunology</subject><subject>B-Lymphocytes - metabolism</subject><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Humans</subject><subject>Immunobiology</subject><subject>Immunoglobulins - biosynthesis</subject><subject>Lymphocyte Activation</subject><subject>Lymphocyte Cooperation</subject><subject>Organs and cells involved in the immune response</subject><subject>Phenotype</subject><subject>Pokeweed Mitogens - pharmacology</subject><subject>T-Lymphocytes - classification</subject><subject>T-Lymphocytes - immunology</subject><subject>T-Lymphocytes, Helper-Inducer - immunology</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkcFu3CAQhlHVKt2kfYKqEoeqOUTeDjYGc4w2bRMpUqVo7whsvCbCsDW2rH2MvnFx1o16AjHffDD8CH0isKVAxbdn2_eTD25LCralW0IofYM2pCwhYwzYW7QByPOMcMbfo8sYnwGAQU4v0EUuirLk-Qb9eTKHyanRBo9Di8_Ggwt6ctbjePJjZ6KNWJ9wN_XK4z2ujXM4TjqaMWIVcWNa602zIMqPNrujFPfBh9oFr9zLmQ7NCc927JIzCfHujt6kQpM21c3iOobj-or4Ab1rlYvm47peof2P7_vdffb46-fD7vYxqymwMROC6LZUNXBTg2YFKzUwLXiu0oyUUEW1EUIbUqhKVIILoUypONAcCFS8uEJfz9rjEH5PJo6yt3EZTXkTpig5Y0LkrEhgcQbrIcQ4mFYeB9ur4SQJyCUH-S8HmXKQVC45pK7Pq37SvWlee9aPT_Uva13FWrl2UL628RXjFeNARMKuz1hnD91sByNjr5xLUiLnef7vwr8ByqEW</recordid><startdate>19860215</startdate><enddate>19860215</enddate><creator>Calvo, CF</creator><creator>Bernard, A</creator><creator>Huet, S</creator><creator>Leroy, E</creator><creator>Boumsell, L</creator><creator>Senik, A</creator><general>Am Assoc Immnol</general><general>American Association of Immunologists</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19860215</creationdate><title>Regulation of immunoglobulin synthesis by human T cell subsets as defined by anti-D44 monoclonal antibody within the CD4+ and CD8+ subpopulations</title><author>Calvo, CF ; Bernard, A ; Huet, S ; Leroy, E ; Boumsell, L ; Senik, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-991bf5ac07ec0b6365b06b972a060414a4be99be13a8989799ae5a7042010873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Analysis of the immune response. Humoral and cellular immunity</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antigen-Antibody Reactions</topic><topic>Antigens, Differentiation, T-Lymphocyte</topic><topic>Antigens, Surface - immunology</topic><topic>B-Lymphocytes - immunology</topic><topic>B-Lymphocytes - metabolism</topic><topic>Biological and medical sciences</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Humans</topic><topic>Immunobiology</topic><topic>Immunoglobulins - biosynthesis</topic><topic>Lymphocyte Activation</topic><topic>Lymphocyte Cooperation</topic><topic>Organs and cells involved in the immune response</topic><topic>Phenotype</topic><topic>Pokeweed Mitogens - pharmacology</topic><topic>T-Lymphocytes - classification</topic><topic>T-Lymphocytes - immunology</topic><topic>T-Lymphocytes, Helper-Inducer - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Calvo, CF</creatorcontrib><creatorcontrib>Bernard, A</creatorcontrib><creatorcontrib>Huet, S</creatorcontrib><creatorcontrib>Leroy, E</creatorcontrib><creatorcontrib>Boumsell, L</creatorcontrib><creatorcontrib>Senik, A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of immunology (1950)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Calvo, CF</au><au>Bernard, A</au><au>Huet, S</au><au>Leroy, E</au><au>Boumsell, L</au><au>Senik, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulation of immunoglobulin synthesis by human T cell subsets as defined by anti-D44 monoclonal antibody within the CD4+ and CD8+ subpopulations</atitle><jtitle>The Journal of immunology (1950)</jtitle><addtitle>J Immunol</addtitle><date>1986-02-15</date><risdate>1986</risdate><volume>136</volume><issue>4</issue><spage>1144</spage><epage>1149</epage><pages>1144-1149</pages><issn>0022-1767</issn><eissn>1550-6606</eissn><coden>JOIMA3</coden><abstract>In our previous paper, we demonstrated that anti-D44 MAb can, in the presence of complement, eliminate all the allocytotoxicity generated during a mixed lymphocyte reaction without affecting the alloproliferative response. As approximately 70% of CD4+ cells and 30% of CD8+ will be stained with anti-D44 MAb, we researched the functional role of the D44+ and D44- cells in each of these T cell subsets in the PWM-induced antibody response. We found that most of the helper activity for immunoglobulin (Ig) synthesis was mediated by CD4+ D44+ lymphocytes and that virtually all the suppressive activity was mediated by CD8+ D44- lymphocytes. Surprisingly enough, we noticed that the low level of Ig synthesis induced in B cells by CD4+ D44- lymphocytes could be strongly amplified by the addition of radiosensitive CD8+ lymphocytes, suggesting coexisting opposite immunoregulatory functions within the CD8+ T cell subset. These results, together with previous data, indicate that anti-D44 MAb subdivides T cells into subpopulations with distinct functional repertoires: a CD4+ D44+ helper subpopulation, a CD8+ D44+ cytotoxic subpopulation, and a CD8+ D44- suppressor subpopulation.</abstract><cop>Bethesda, MD</cop><pub>Am Assoc Immnol</pub><pmid>2935572</pmid><doi>10.4049/jimmunol.136.4.1144</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-1767 |
| ispartof | The Journal of immunology (1950), 1986-02, Vol.136 (4), p.1144-1149 |
| issn | 0022-1767 1550-6606 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76699263 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Analysis of the immune response. Humoral and cellular immunity Antibodies, Monoclonal - immunology Antigen-Antibody Reactions Antigens, Differentiation, T-Lymphocyte Antigens, Surface - immunology B-Lymphocytes - immunology B-Lymphocytes - metabolism Biological and medical sciences Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Immunobiology Immunoglobulins - biosynthesis Lymphocyte Activation Lymphocyte Cooperation Organs and cells involved in the immune response Phenotype Pokeweed Mitogens - pharmacology T-Lymphocytes - classification T-Lymphocytes - immunology T-Lymphocytes, Helper-Inducer - immunology |
| title | Regulation of immunoglobulin synthesis by human T cell subsets as defined by anti-D44 monoclonal antibody within the CD4+ and CD8+ subpopulations |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-08T16%3A56%3A23IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Regulation%20of%20immunoglobulin%20synthesis%20by%20human%20T%20cell%20subsets%20as%20defined%20by%20anti-D44%20monoclonal%20antibody%20within%20the%20CD4+%20and%20CD8+%20subpopulations&rft.jtitle=The%20Journal%20of%20immunology%20(1950)&rft.au=Calvo,%20CF&rft.date=1986-02-15&rft.volume=136&rft.issue=4&rft.spage=1144&rft.epage=1149&rft.pages=1144-1149&rft.issn=0022-1767&rft.eissn=1550-6606&rft.coden=JOIMA3&rft_id=info:doi/10.4049/jimmunol.136.4.1144&rft_dat=%3Cproquest_cross%3E76699263%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=76699263&rft_id=info:pmid/2935572&rfr_iscdi=true |