Cytophotometric Analysis of Neuronal Chromatin and RNA Changes in Oxotremorine-Treated Rats
Abstract Neuronal nucleic acid responses were examined within the rat striatum and sensorimotor cortex (layer V) following single intraperitoneal injections of the central cholinergic-muscarinic agonist oxotremorine (0.1, 0.7, or 1.0 mg/kg). After stoichiometric Feulgen and azure B staining of brain...
Gespeichert in:
| Veröffentlicht in: | Experimental biology and medicine (Maywood, N.J.) N.J.), 1986-01, Vol.181 (1), p.41-48 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 48 |
|---|---|
| container_issue | 1 |
| container_start_page | 41 |
| container_title | Experimental biology and medicine (Maywood, N.J.) |
| container_volume | 181 |
| creator | Martin, Lee J. Doebler, Jeffrey A. Anthony, Adam |
| description | Abstract
Neuronal nucleic acid responses were examined within the rat striatum and sensorimotor cortex (layer V) following single intraperitoneal injections of the central cholinergic-muscarinic agonist oxotremorine (0.1, 0.7, or 1.0 mg/kg). After stoichiometric Feulgen and azure B staining of brain sections, scanning-integrating microdensitometry was used to quantify Feulgen-deoxyribonucleic acid levels, changes in the susceptibility of chromatin to Feulgen acid hydrolysis (F-DNA yield) and azure B-ribonucleic acid (RNA) content of neurons on an individual basis. Changes in neuronal nuclear and nucleolar volumes were also determined histometrically. Within the striatum and sensorimotor cortex, oxotremorine produced marked dose-dependent elevations in both F-DNA yield and RNA content. These metabolic increases were typically paralleled by elevations in nuclear and nucleolar volumes. The data demonstrate that the oxotremorine-induced central muscarinic activation is associated with dose-related enhancements in neuronal chromatin template activity, RNA content, and protein synthetic capacity. |
| doi_str_mv | 10.3181/00379727-181-42222 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_76697920</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sage_id>10.3181_00379727-181-42222</sage_id><sourcerecordid>76697920</sourcerecordid><originalsourceid>FETCH-LOGICAL-c367t-23ddde33b3c4432a99d0646a903675ac81f5bf4bd53516f3d92093ec491bc23e3</originalsourceid><addsrcrecordid>eNp9kF1LwzAYhYMoc07_gCD0Qryry1fT5XIMv2BsIPPKi5Kmb7eOtplJCu7fm7q5S3MTznue84YchG4JfmRkQsYYs1SmNI2DiDkN5wwNScKSmAkpz9GwB-KeuERXzm0xJklKxQANKCcTzpMh-pztvdltjDcNeFvpaNqqeu8qF5kyWkBnTdDRbGNNo3zVRqotovfFNExUuwYXhdHy23gLjbFVC_HKgvIQGOXdNbooVe3g5niP0Mfz02r2Gs-XL2-z6TzWTKQ-pqwoCmAsZ5pzRpWUBRZcKImDnSg9IWWSlzwvwseIKFkhKZYMNJck15QBG6GHw96dNV8dOJ81ldNQ16oF07ksFUKmIRRAegC1Nc5ZKLOdrRpl9xnBWd9o9tdo1ovfRkPo7ri9yxsoTpFjhcG_P_rKaVWXVrW6cicslRPBGQ_Y-IA5tYZsazobinX_PfwDndmLNg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>76697920</pqid></control><display><type>article</type><title>Cytophotometric Analysis of Neuronal Chromatin and RNA Changes in Oxotremorine-Treated Rats</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Martin, Lee J. ; Doebler, Jeffrey A. ; Anthony, Adam</creator><creatorcontrib>Martin, Lee J. ; Doebler, Jeffrey A. ; Anthony, Adam</creatorcontrib><description>Abstract
Neuronal nucleic acid responses were examined within the rat striatum and sensorimotor cortex (layer V) following single intraperitoneal injections of the central cholinergic-muscarinic agonist oxotremorine (0.1, 0.7, or 1.0 mg/kg). After stoichiometric Feulgen and azure B staining of brain sections, scanning-integrating microdensitometry was used to quantify Feulgen-deoxyribonucleic acid levels, changes in the susceptibility of chromatin to Feulgen acid hydrolysis (F-DNA yield) and azure B-ribonucleic acid (RNA) content of neurons on an individual basis. Changes in neuronal nuclear and nucleolar volumes were also determined histometrically. Within the striatum and sensorimotor cortex, oxotremorine produced marked dose-dependent elevations in both F-DNA yield and RNA content. These metabolic increases were typically paralleled by elevations in nuclear and nucleolar volumes. The data demonstrate that the oxotremorine-induced central muscarinic activation is associated with dose-related enhancements in neuronal chromatin template activity, RNA content, and protein synthetic capacity.</description><identifier>ISSN: 0037-9727</identifier><identifier>ISSN: 1535-3702</identifier><identifier>EISSN: 1535-3699</identifier><identifier>EISSN: 1525-1373</identifier><identifier>DOI: 10.3181/00379727-181-42222</identifier><identifier>PMID: 2418445</identifier><identifier>CODEN: PSEBAA</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Animals ; Biological and medical sciences ; Chromatin - drug effects ; Cytophotometry ; DNA - analysis ; Drug toxicity and drugs side effects treatment ; Glucosephosphate Dehydrogenase - analysis ; Male ; Medical sciences ; Neurons - analysis ; Neurons - drug effects ; Oxotremorine - pharmacology ; Pharmacology. Drug treatments ; Rats ; Rats, Inbred Strains ; Receptors, Muscarinic - drug effects ; RNA - analysis ; Toxicity: nervous system and muscle</subject><ispartof>Experimental biology and medicine (Maywood, N.J.), 1986-01, Vol.181 (1), p.41-48</ispartof><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c367t-23ddde33b3c4432a99d0646a903675ac81f5bf4bd53516f3d92093ec491bc23e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7986434$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2418445$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Martin, Lee J.</creatorcontrib><creatorcontrib>Doebler, Jeffrey A.</creatorcontrib><creatorcontrib>Anthony, Adam</creatorcontrib><title>Cytophotometric Analysis of Neuronal Chromatin and RNA Changes in Oxotremorine-Treated Rats</title><title>Experimental biology and medicine (Maywood, N.J.)</title><addtitle>Proc Soc Exp Biol Med</addtitle><description>Abstract
Neuronal nucleic acid responses were examined within the rat striatum and sensorimotor cortex (layer V) following single intraperitoneal injections of the central cholinergic-muscarinic agonist oxotremorine (0.1, 0.7, or 1.0 mg/kg). After stoichiometric Feulgen and azure B staining of brain sections, scanning-integrating microdensitometry was used to quantify Feulgen-deoxyribonucleic acid levels, changes in the susceptibility of chromatin to Feulgen acid hydrolysis (F-DNA yield) and azure B-ribonucleic acid (RNA) content of neurons on an individual basis. Changes in neuronal nuclear and nucleolar volumes were also determined histometrically. Within the striatum and sensorimotor cortex, oxotremorine produced marked dose-dependent elevations in both F-DNA yield and RNA content. These metabolic increases were typically paralleled by elevations in nuclear and nucleolar volumes. The data demonstrate that the oxotremorine-induced central muscarinic activation is associated with dose-related enhancements in neuronal chromatin template activity, RNA content, and protein synthetic capacity.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Chromatin - drug effects</subject><subject>Cytophotometry</subject><subject>DNA - analysis</subject><subject>Drug toxicity and drugs side effects treatment</subject><subject>Glucosephosphate Dehydrogenase - analysis</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Neurons - analysis</subject><subject>Neurons - drug effects</subject><subject>Oxotremorine - pharmacology</subject><subject>Pharmacology. Drug treatments</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Receptors, Muscarinic - drug effects</subject><subject>RNA - analysis</subject><subject>Toxicity: nervous system and muscle</subject><issn>0037-9727</issn><issn>1535-3702</issn><issn>1535-3699</issn><issn>1525-1373</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kF1LwzAYhYMoc07_gCD0Qryry1fT5XIMv2BsIPPKi5Kmb7eOtplJCu7fm7q5S3MTznue84YchG4JfmRkQsYYs1SmNI2DiDkN5wwNScKSmAkpz9GwB-KeuERXzm0xJklKxQANKCcTzpMh-pztvdltjDcNeFvpaNqqeu8qF5kyWkBnTdDRbGNNo3zVRqotovfFNExUuwYXhdHy23gLjbFVC_HKgvIQGOXdNbooVe3g5niP0Mfz02r2Gs-XL2-z6TzWTKQ-pqwoCmAsZ5pzRpWUBRZcKImDnSg9IWWSlzwvwseIKFkhKZYMNJck15QBG6GHw96dNV8dOJ81ldNQ16oF07ksFUKmIRRAegC1Nc5ZKLOdrRpl9xnBWd9o9tdo1ovfRkPo7ri9yxsoTpFjhcG_P_rKaVWXVrW6cicslRPBGQ_Y-IA5tYZsazobinX_PfwDndmLNg</recordid><startdate>198601</startdate><enddate>198601</enddate><creator>Martin, Lee J.</creator><creator>Doebler, Jeffrey A.</creator><creator>Anthony, Adam</creator><general>SAGE Publications</general><general>Blackwell Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198601</creationdate><title>Cytophotometric Analysis of Neuronal Chromatin and RNA Changes in Oxotremorine-Treated Rats</title><author>Martin, Lee J. ; Doebler, Jeffrey A. ; Anthony, Adam</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c367t-23ddde33b3c4432a99d0646a903675ac81f5bf4bd53516f3d92093ec491bc23e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Chromatin - drug effects</topic><topic>Cytophotometry</topic><topic>DNA - analysis</topic><topic>Drug toxicity and drugs side effects treatment</topic><topic>Glucosephosphate Dehydrogenase - analysis</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Neurons - analysis</topic><topic>Neurons - drug effects</topic><topic>Oxotremorine - pharmacology</topic><topic>Pharmacology. Drug treatments</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Receptors, Muscarinic - drug effects</topic><topic>RNA - analysis</topic><topic>Toxicity: nervous system and muscle</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Martin, Lee J.</creatorcontrib><creatorcontrib>Doebler, Jeffrey A.</creatorcontrib><creatorcontrib>Anthony, Adam</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Martin, Lee J.</au><au>Doebler, Jeffrey A.</au><au>Anthony, Adam</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytophotometric Analysis of Neuronal Chromatin and RNA Changes in Oxotremorine-Treated Rats</atitle><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle><addtitle>Proc Soc Exp Biol Med</addtitle><date>1986-01</date><risdate>1986</risdate><volume>181</volume><issue>1</issue><spage>41</spage><epage>48</epage><pages>41-48</pages><issn>0037-9727</issn><issn>1535-3702</issn><eissn>1535-3699</eissn><eissn>1525-1373</eissn><coden>PSEBAA</coden><abstract>Abstract
Neuronal nucleic acid responses were examined within the rat striatum and sensorimotor cortex (layer V) following single intraperitoneal injections of the central cholinergic-muscarinic agonist oxotremorine (0.1, 0.7, or 1.0 mg/kg). After stoichiometric Feulgen and azure B staining of brain sections, scanning-integrating microdensitometry was used to quantify Feulgen-deoxyribonucleic acid levels, changes in the susceptibility of chromatin to Feulgen acid hydrolysis (F-DNA yield) and azure B-ribonucleic acid (RNA) content of neurons on an individual basis. Changes in neuronal nuclear and nucleolar volumes were also determined histometrically. Within the striatum and sensorimotor cortex, oxotremorine produced marked dose-dependent elevations in both F-DNA yield and RNA content. These metabolic increases were typically paralleled by elevations in nuclear and nucleolar volumes. The data demonstrate that the oxotremorine-induced central muscarinic activation is associated with dose-related enhancements in neuronal chromatin template activity, RNA content, and protein synthetic capacity.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>2418445</pmid><doi>10.3181/00379727-181-42222</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0037-9727 |
| ispartof | Experimental biology and medicine (Maywood, N.J.), 1986-01, Vol.181 (1), p.41-48 |
| issn | 0037-9727 1535-3702 1535-3699 1525-1373 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76697920 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Biological and medical sciences Chromatin - drug effects Cytophotometry DNA - analysis Drug toxicity and drugs side effects treatment Glucosephosphate Dehydrogenase - analysis Male Medical sciences Neurons - analysis Neurons - drug effects Oxotremorine - pharmacology Pharmacology. Drug treatments Rats Rats, Inbred Strains Receptors, Muscarinic - drug effects RNA - analysis Toxicity: nervous system and muscle |
| title | Cytophotometric Analysis of Neuronal Chromatin and RNA Changes in Oxotremorine-Treated Rats |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-10T02%3A29%3A35IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cytophotometric%20Analysis%20of%20Neuronal%20Chromatin%20and%20RNA%20Changes%20in%20Oxotremorine-Treated%20Rats&rft.jtitle=Experimental%20biology%20and%20medicine%20(Maywood,%20N.J.)&rft.au=Martin,%20Lee%20J.&rft.date=1986-01&rft.volume=181&rft.issue=1&rft.spage=41&rft.epage=48&rft.pages=41-48&rft.issn=0037-9727&rft.eissn=1535-3699&rft.coden=PSEBAA&rft_id=info:doi/10.3181/00379727-181-42222&rft_dat=%3Cproquest_cross%3E76697920%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=76697920&rft_id=info:pmid/2418445&rft_sage_id=10.3181_00379727-181-42222&rfr_iscdi=true |