Purification and characterization of two new c‐type cytochromes involved in Fe2+ oxidation from Thiobacillus ferrooxidans
Two new c‐type cytochromes have been purified from cell membranes of the acidophilic Thiobacillus ferrooxidans. In contrast to a soluble cytochrome c with molecular mass of 14 kDa reported earlier, a membrane‐bound cytochrome c with a mass of 21 kDa was solubilized with octylthioglucoside and purifi...
Gespeichert in:
| Veröffentlicht in: | FEMS microbiology letters 1994-08, Vol.121 (1), p.61-69 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 69 |
|---|---|
| container_issue | 1 |
| container_start_page | 61 |
| container_title | FEMS microbiology letters |
| container_volume | 121 |
| creator | Valkova‐Valchanova, Maria B. Chan, Samuel H.P. |
| description | Two new c‐type cytochromes have been purified from cell membranes of the acidophilic Thiobacillus ferrooxidans. In contrast to a soluble cytochrome c with molecular mass of 14 kDa reported earlier, a membrane‐bound cytochrome c with a mass of 21 kDa was solubilized with octylthioglucoside and purified to homogeneity. In addition, a high molecular mass c‐type cytochrome (68 kDa) was also solubilized and purified using Triton X‐100 as a detergent. Both acid‐stable species are partially released during osmotic shock and chloroform treatment of the bacteria; they are integral components in the respiratory chain donating electrons to the terminal cytochrome oxidase. |
| doi_str_mv | 10.1111/j.1574-6968.1994.tb07076.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_76695970</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>76695970</sourcerecordid><originalsourceid>FETCH-LOGICAL-p1361-9bfecf9528ed32d0cfca2fe2a658596235da9296cd54a5f13eac296b9ea4ce2e3</originalsourceid><addsrcrecordid>eNo9kcGO0zAQhi0EWsrCIyBZCHFZJdhOYscHDqsVBaQiOCxna-KMVVdpXOxk28JlH4Fn5ElIaVRfxp7_mzn4I-QNZzmfzvtNzitVZlLLOudal_nQMMWUzA9PyOISPSULVqg640yr5-RFShvGWCmYvCJXNatFLdSC_P4-Ru-8hcGHnkLfUruGCHbA6H-dm8HRYR9oj3tq_z7-GY47pPY4BLuOYYuJ-v4hdA_YThe6RHFDw8G351E3EfR-7UMD1nfdmKjDGMN_oE8vyTMHXcJXc70mP5Yf7-8-Z6tvn77c3a6yHS8kz3Tj0DpdiRrbQrTMOgvCoQBZ1ZWWoqha0EJL21YlVI4XCHZ6NhqhtCiwuCbvznt3MfwcMQ1m65PFroMew5iMklJXWrEJfD2DY7PF1uyi30I8mvm3pvztnEOy0LkIvfXpgpVCKMbLCftwxva-w-Ml5syc7JmNOSkyJ0XmZM_M9szBLL-uJC_-AZsMkqM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>76695970</pqid></control><display><type>article</type><title>Purification and characterization of two new c‐type cytochromes involved in Fe2+ oxidation from Thiobacillus ferrooxidans</title><source>MEDLINE</source><source>Oxford University Press Journals Digital Archive Legacy</source><source>Access via Wiley Online Library</source><source>Alma/SFX Local Collection</source><creator>Valkova‐Valchanova, Maria B. ; Chan, Samuel H.P.</creator><creatorcontrib>Valkova‐Valchanova, Maria B. ; Chan, Samuel H.P.</creatorcontrib><description>Two new c‐type cytochromes have been purified from cell membranes of the acidophilic Thiobacillus ferrooxidans. In contrast to a soluble cytochrome c with molecular mass of 14 kDa reported earlier, a membrane‐bound cytochrome c with a mass of 21 kDa was solubilized with octylthioglucoside and purified to homogeneity. In addition, a high molecular mass c‐type cytochrome (68 kDa) was also solubilized and purified using Triton X‐100 as a detergent. Both acid‐stable species are partially released during osmotic shock and chloroform treatment of the bacteria; they are integral components in the respiratory chain donating electrons to the terminal cytochrome oxidase.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.1994.tb07076.x</identifier><identifier>PMID: 8082827</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Amino Acids - analysis ; Azurin - analogs & derivatives ; Azurin - pharmacology ; Bacteriology ; Biological and medical sciences ; Cations, Divalent - metabolism ; Cell Compartmentation ; Cytochrome c ; Cytochrome c Group - isolation & purification ; Cytochrome c Group - metabolism ; Cytochrome oxidase ; Fundamental and applied biological sciences. Psychology ; Iron - metabolism ; Metabolism. Enzymes ; Microbiology ; Molecular Weight ; Oxidation-Reduction - drug effects ; Spectrophotometry ; Thiobacillus - enzymology ; Thiobacillus ferrooxidans</subject><ispartof>FEMS microbiology letters, 1994-08, Vol.121 (1), p.61-69</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6968.1994.tb07076.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6968.1994.tb07076.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4227014$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8082827$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Valkova‐Valchanova, Maria B.</creatorcontrib><creatorcontrib>Chan, Samuel H.P.</creatorcontrib><title>Purification and characterization of two new c‐type cytochromes involved in Fe2+ oxidation from Thiobacillus ferrooxidans</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Two new c‐type cytochromes have been purified from cell membranes of the acidophilic Thiobacillus ferrooxidans. In contrast to a soluble cytochrome c with molecular mass of 14 kDa reported earlier, a membrane‐bound cytochrome c with a mass of 21 kDa was solubilized with octylthioglucoside and purified to homogeneity. In addition, a high molecular mass c‐type cytochrome (68 kDa) was also solubilized and purified using Triton X‐100 as a detergent. Both acid‐stable species are partially released during osmotic shock and chloroform treatment of the bacteria; they are integral components in the respiratory chain donating electrons to the terminal cytochrome oxidase.</description><subject>Amino Acids - analysis</subject><subject>Azurin - analogs & derivatives</subject><subject>Azurin - pharmacology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Cations, Divalent - metabolism</subject><subject>Cell Compartmentation</subject><subject>Cytochrome c</subject><subject>Cytochrome c Group - isolation & purification</subject><subject>Cytochrome c Group - metabolism</subject><subject>Cytochrome oxidase</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Iron - metabolism</subject><subject>Metabolism. Enzymes</subject><subject>Microbiology</subject><subject>Molecular Weight</subject><subject>Oxidation-Reduction - drug effects</subject><subject>Spectrophotometry</subject><subject>Thiobacillus - enzymology</subject><subject>Thiobacillus ferrooxidans</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kcGO0zAQhi0EWsrCIyBZCHFZJdhOYscHDqsVBaQiOCxna-KMVVdpXOxk28JlH4Fn5ElIaVRfxp7_mzn4I-QNZzmfzvtNzitVZlLLOudal_nQMMWUzA9PyOISPSULVqg640yr5-RFShvGWCmYvCJXNatFLdSC_P4-Ru-8hcGHnkLfUruGCHbA6H-dm8HRYR9oj3tq_z7-GY47pPY4BLuOYYuJ-v4hdA_YThe6RHFDw8G351E3EfR-7UMD1nfdmKjDGMN_oE8vyTMHXcJXc70mP5Yf7-8-Z6tvn77c3a6yHS8kz3Tj0DpdiRrbQrTMOgvCoQBZ1ZWWoqha0EJL21YlVI4XCHZ6NhqhtCiwuCbvznt3MfwcMQ1m65PFroMew5iMklJXWrEJfD2DY7PF1uyi30I8mvm3pvztnEOy0LkIvfXpgpVCKMbLCftwxva-w-Ml5syc7JmNOSkyJ0XmZM_M9szBLL-uJC_-AZsMkqM</recordid><startdate>199408</startdate><enddate>199408</enddate><creator>Valkova‐Valchanova, Maria B.</creator><creator>Chan, Samuel H.P.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>199408</creationdate><title>Purification and characterization of two new c‐type cytochromes involved in Fe2+ oxidation from Thiobacillus ferrooxidans</title><author>Valkova‐Valchanova, Maria B. ; Chan, Samuel H.P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p1361-9bfecf9528ed32d0cfca2fe2a658596235da9296cd54a5f13eac296b9ea4ce2e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Amino Acids - analysis</topic><topic>Azurin - analogs & derivatives</topic><topic>Azurin - pharmacology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Cations, Divalent - metabolism</topic><topic>Cell Compartmentation</topic><topic>Cytochrome c</topic><topic>Cytochrome c Group - isolation & purification</topic><topic>Cytochrome c Group - metabolism</topic><topic>Cytochrome oxidase</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Iron - metabolism</topic><topic>Metabolism. Enzymes</topic><topic>Microbiology</topic><topic>Molecular Weight</topic><topic>Oxidation-Reduction - drug effects</topic><topic>Spectrophotometry</topic><topic>Thiobacillus - enzymology</topic><topic>Thiobacillus ferrooxidans</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Valkova‐Valchanova, Maria B.</creatorcontrib><creatorcontrib>Chan, Samuel H.P.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Valkova‐Valchanova, Maria B.</au><au>Chan, Samuel H.P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Purification and characterization of two new c‐type cytochromes involved in Fe2+ oxidation from Thiobacillus ferrooxidans</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>1994-08</date><risdate>1994</risdate><volume>121</volume><issue>1</issue><spage>61</spage><epage>69</epage><pages>61-69</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Two new c‐type cytochromes have been purified from cell membranes of the acidophilic Thiobacillus ferrooxidans. In contrast to a soluble cytochrome c with molecular mass of 14 kDa reported earlier, a membrane‐bound cytochrome c with a mass of 21 kDa was solubilized with octylthioglucoside and purified to homogeneity. In addition, a high molecular mass c‐type cytochrome (68 kDa) was also solubilized and purified using Triton X‐100 as a detergent. Both acid‐stable species are partially released during osmotic shock and chloroform treatment of the bacteria; they are integral components in the respiratory chain donating electrons to the terminal cytochrome oxidase.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>8082827</pmid><doi>10.1111/j.1574-6968.1994.tb07076.x</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 1994-08, Vol.121 (1), p.61-69 |
| issn | 0378-1097 1574-6968 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76695970 |
| source | MEDLINE; Oxford University Press Journals Digital Archive Legacy; Access via Wiley Online Library; Alma/SFX Local Collection |
| subjects | Amino Acids - analysis Azurin - analogs & derivatives Azurin - pharmacology Bacteriology Biological and medical sciences Cations, Divalent - metabolism Cell Compartmentation Cytochrome c Cytochrome c Group - isolation & purification Cytochrome c Group - metabolism Cytochrome oxidase Fundamental and applied biological sciences. Psychology Iron - metabolism Metabolism. Enzymes Microbiology Molecular Weight Oxidation-Reduction - drug effects Spectrophotometry Thiobacillus - enzymology Thiobacillus ferrooxidans |
| title | Purification and characterization of two new c‐type cytochromes involved in Fe2+ oxidation from Thiobacillus ferrooxidans |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T14%3A51%3A09IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Purification%20and%20characterization%20of%20two%20new%20c%E2%80%90type%20cytochromes%20involved%20in%20Fe2+%20oxidation%20from%20Thiobacillus%20ferrooxidans&rft.jtitle=FEMS%20microbiology%20letters&rft.au=Valkova%E2%80%90Valchanova,%20Maria%20B.&rft.date=1994-08&rft.volume=121&rft.issue=1&rft.spage=61&rft.epage=69&rft.pages=61-69&rft.issn=0378-1097&rft.eissn=1574-6968&rft.coden=FMLED7&rft_id=info:doi/10.1111/j.1574-6968.1994.tb07076.x&rft_dat=%3Cproquest_pubme%3E76695970%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=76695970&rft_id=info:pmid/8082827&rfr_iscdi=true |