A Simplified Method for Passage and Long-Term Growth of Human Mammary Epithelial Cells

A method is described for culturing human mammary epithelial cells in primary culture and allowing more than 50 generations and a 1000-fold increase from starting inocula without need of enzymatic transfers. Organoids dissociated from breast tissue are plated in medium containing$1.05 mM Ca^{++}$to...

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Veröffentlicht in:	In Vitro Cellular & Developmental Biology 1986-01, Vol.22 (1), p.6-12
Hauptverfasser:	SOULE, H. D, MCGRATH, C. M
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal cells Biological and medical sciences Biotechnology Breast - metabolism Breast - pathology Breast - ultrastructure Breast Neoplasms - pathology Calcium - pharmacology Cell culture techniques Cell cultures. Hybridization. Fusion Cell Division - drug effects Cell growth Cell Survival - drug effects Cells, Cultured Chromosomes - ultrastructure Collagens Culture Media - pharmacology Culture Techniques - methods Cultured cells Epithelial cells Epithelium Epithelium - metabolism Epithelium - pathology Epithelium - ultrastructure Establishment of new cell lines, improvement of cultural methods, mass cultures Eukaryotic cell cultures Female Fundamental and applied biological sciences. Psychology Humans Keratins - biosynthesis Longevity Mastectomy Methods. Procedures. Technologies Molecular and cellular biology Organoids Stromal cells
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